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3 Biotech

, 8:476 | Cite as

Transcriptional activation of anthocyanin structural genes in Torenia ‘Kauai Rose’ via overexpression of anthocyanin regulatory transcription factors

  • Xu Junping
  • Aung Htay Naing
  • Chang Kil Kim
Short Reports
  • 17 Downloads

Abstract

This study was conducted to examine the role of the transcription factors (TFs), RsMYB1 and mPAP1 together with B-Peru (mPAP1 + B-Peru), in regulating anthocyanin biosynthesis in the ornamental torenia (Torenia fournieri) cultivar Kauai Rose using Agrobacterium-mediated transformation. Expression levels of RsMYB1 were the highest in the lines RS5 and RS3, followed by RS4, RS2, and RS1, while transcript levels of mPAP1 + B-Peru increased in the order of PB-6 > PB-5 > PB-7 > PB-8 > PB-2. Moreover, transcript levels of the anthocyanin structural genes in transgenic lines were significantly higher than those in wild-type (WT) plants. Anthocyanin structural gene expression was specifically altered by TF overexpression: the highest expression of anthocyanidin synthase (ANS) was observed in transgenic lines with RsMYB1, while expression of dihydroflavonol-4-reductase (DFR) was the highest in lines with mPAP1 + B-Peru. We expect that enhanced expression of these anthocyanin structural genes will improve anthocyanin content in the flowers of transgenic torenia. Moreover, these results indicate that RsMYB1 and mPAP1 + B-Peru can be exploited as anthocyanin regulatory TFs to enhance anthocyanin content in other horticultural plants.

Keywords

Flower color Genetic transformation Ornamental plant Quantitative real-time polymerase chain reaction (qRT-PCR) Anthocyanin RsMYB1 mPAP1 B-Peru 

Notes

Acknowledgements

We wish to acknowledge Dr. Sun-Hung Lim (RDA, South Korea) for providing the plasmid constructs.

Author contributions

AHN and XJ designed the study, conducted the experiments, and wrote the manuscript. CKK supervised experiments at all stages and performed critical revisions of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest.

Supplementary material

13205_2018_1505_MOESM1_ESM.docx (73 kb)
Supplementary material 1 (DOCX 73 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Horticultural ScienceKyungpook National UniversityDaeguSouth Korea

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