High-throughput sequencing analysis of Euphorbia fischeriana Steud provides insights into the molecular mechanism of pharmaceutical ingredient biosynthesis
- 6 Downloads
High-throughput sequencing is an effective approach to analyse the bioinformation on the molecular biological and whole genome levels, especially in non-model plants for which reference genome sequences are unavailable. In this study, high-throughput sequencing analysis of Euphorbia fischeriana Steud was conducted on the Illumina HiSeq 2000 platform. A total of 9,6481,893 raw reads were generated and assembled into 304,217 transcripts and 186,384 unigenes. Of the 186,384 unigenes, 77.45% were annotated in at least one database, and some pathways involved in the biosynthesis of the terpenoid backbone were closely linked to the main anticancer components. In addition, 7452 transcription factors and 76,193 SSRs were detected. This study may provide a candidate pathway for terpenoid backbone biosynthesis in this medicinal plant.
KeywordsEuphorbia fischeriana Steud High-throughput sequencing analysis Diterpenoid biosynthesis
This work was supported by the Fundamental Research Funds for Education Department of Heilongjiang Province (no. 2016-KYYWF-0869).
Compliance with ethical standards
This article does not include any studies with human participants or animals performed by any of the authors.
This article does not involve any informed consent.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bretagne S, Costa JM, Besmond C, Carsique R, Calderone R (1997) Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as basis for typing system. J Clin Microbiol 35:1777–1780. http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC229840&blobtype=pdf
- Boer KD, Tilleman S, Pauwels L, Vanden RB, De VS, Vanderhaeghen R, Hilson P, Hanmill JD, Goossens A (2011) Apetala2/ethylene response factor and basic helix-loop-helix tobacco transcription factors cooperatively mediate jasmonate-elicited nicotine biosynthesis. Plant J 66:1053–1065. https://doi.org/10.1111/j.1365-313X.2011.04566.x CrossRefPubMedGoogle Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng QD, Chen ZH, Mauceli E, Hacohen N, Gnirke A, Rhind N, Palma FD, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644–652. https://www.nature.com/articles/nbt.1883.ris CrossRefGoogle Scholar
- Kirby J, Nishimoto M, Park JG, Withers ST, Nowroozi F, Behrendt D, Rutledge EJ, Fortman JL, Johnson HE, Anderson JV, Keasling JD (2010) Cloning of casbene and neocembrene synthases from Euphorbiaceae plants and expression in Saccharomyces cerevisiae. Phytochemistry 71:1466–1473. https://doi.org/10.1016/j.phytochem.2010.06.001 CrossRefPubMedGoogle Scholar
- Li W, Li L, Chen ZJ (2014) Genetic diversity of germplasm resources of Euphorbia Fischeriana Steud based on ISSR makers. J Liaoning Univ Tradit Chin Med. 16:50–52. https://doi.org/10.13194/j.issn.1673-842x.2014.03.018 (in Chinese) CrossRefGoogle Scholar
- Liu TM, Zeng LB, Zhu SY, Chen XJ, Tang QM, Mei SY, Tang SW (2015) Large-scale development of expressed sequence tag-derived simple sequence repeat markers by deep transcriptome sequencing in garlic (Allium sativum L.). Mol Breed 35:204–212. https://doi.org/10.1007/s11032-015-0399-x CrossRefGoogle Scholar
- Ma DM, Pu GB, Lei CY, Ma LQ, Wang HH, Guo YW, Chen JL, Du ZG, Wang H, Li GF, Ye HC, Liu BY (2009) Isolation and characterization of AaWRKY1, an Artemisia annua transcription factor that regulates the amorpha-4, 11-diene synthase gene, a key gene of artemisinin biosynthesis. Plant Cell Physiol 50:2146–2161. https://doi.org/10.1093/pcp/pcp149 CrossRefPubMedGoogle Scholar
- Shen L, Zhang SQ, Liu L, Sun Y, Wu YX, Xie LP, Liu JC (2017) Jolkinolide A and jolkinolide B inhibit proliferation of A549 cells and activity of human umbilical vein endothelial cells. Med Sci Monit 23:223–237. https://www.medscimonit.com/abstract/index/idArt/902704 CrossRefGoogle Scholar
- Wang Y, Ma XQ, Yan SS, Shen SS, Zhu HL, Gu Y, Wang HB, Qin GW, Yu Q (2009) 17-Hydroxy-jolkinolide B inhibits signal transducers and activators of transcription 3 signaling by covalently cross-linking Janus Kinases and induces apoptosis of human cancer cells. Cancer Res 69:7302–7310. https://doi.org/10.1158/0008-5472.CAN-09-0462 CrossRefPubMedGoogle Scholar
- Zhang B, Zhang W, Nie RE, Li WZ, Segraves KA, Yang XK, Xue HJ (2016) Comparative transcriptome analysis of chemosensory genes in two sister leaf beetles provides insights into chemosensory speciation. Insect Biochem Mol Biol 79:108–118. https://doi.org/10.1016/j.ibmb.2016.11.001 CrossRefPubMedGoogle Scholar