Genome-wide overview of Trichosporon akiyoshidainum HP-2023, new insights into its mechanism of dye discoloration
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Trichosporon akiyoshidainum HP-2023 completely discolorised Reactive Black 5 (200 mg/l) in 24 h. Manganese peroxidase and phenoloxidase, but no laccase activities were detected throughout the incubation. Total aromatic amines in media with Reactive Black 5 decreased 83% after 24 h, supporting an oxidative mechanism of azo dye discoloration. To unravel the genetic basis of these activities, the genome of Trichosporon akiyoshidainum HP-2023 was sequenced, assembled and annotated de novo. T. akiyoshidainum HP-2023 genome comprises 30 MB with a G+C content of 60.75% and 9019 gene models. Thirty-three putative carbohydrate-active enzymes with auxiliary activities, probably involved in lignin degradation and dye discoloration, were identified in the annotated genome, including two laccases, four extracellular fungal heme-peroxidases, nineteen hydrogen peroxide-producing enzymes, and four benzoquinone oxidoreductases. This report will facilitate further studies of textile-dye discoloration with this and closely related strains and poses questions about the ligninolytic potential of Trichosporon akiyoshidainum HP-2023 and related species.
KeywordsTrichosporon akiyoshidainum Genome Bio-discoloration mechanism Yeasts
Author contribution statement
NBG and MRS performed the cultivation, DNA extraction and genome comparison of selected genomes. HFP provided T. akiyoshidainum HP-2023, designed the experiments, and contributed to the writing of the manuscript. LICF supervised the project and reviewed the manuscript, while DGK annotated T. akiyoshidainum HP-2023 genome, provided computational support and contributed to the writing of the manuscript. All authors read and approved the final manuscript.
This research was supported by the Agencia Nacional de Promoción Científica Y Tecnológica (Grants Numbers PICT 1154-2013 and PICT 2884-2014). The genome of T. oleaginosus and their ESTs data were produced by the US Department of Energy Joint Genome Institute http://www.jgi.doe.gov/ in collaboration with the user community.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest in the publication.
This study focused on the analysis of a genome sequence of the cellulolytic fungus Trichosporon akiyoshidainum HP-2023. This article does not describe any studies involving human participants or animals performed by any of the authors. Our manuscript complies with the Ethical Rules applicable for 3 Biotech.
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