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3 Biotech

, 8:439 | Cite as

In vitro antibacterial and early stage biofilm inhibitory potential of an edible chitosan and its phenolic conjugates against Pseudomonas aeruginosa and Listeria monocytogenes

  • Gabjin Kim
  • Chakradhar Dasagrandhi
  • Eun-Hye Kang
  • Sung-Hwan Eom
  • Young-Mog Kim
Short Reports

Abstract

In the present study, the antibacterial potential of chitosan grafted with phenolics (CPCs) such as caffeic acid (CCA), ferulic (CFA), and sinapic acid (CSA) were evaluated against foodborne pathogens like Pseudomonas aeruginosa (PA) and Listeria monocytogenes (LM). The geometric means of minimum inhibitory concentration (MIC range 0.05–0.33 mg/ml), bactericidal concentration (MBC range 0.30–0.45 mg/ml), biofilm inhibitory concentration (BIC range 0.42–0.83 mg/ml), and biofilm eradication concentration (BEC range 1.71–3.70 mg/ml) of CPCs were found to be lower than the MIC (0.12–1.08 mg/ml), MBC (0.17–1.84 mg/ml), BIC (4.0–4.50 mg/ml), and BEC (17.4–23.0 mg/ml) of unmodified chitosan against PA and LM. CPCs attenuated the biofilms of PA and LM by increasing the membrane permeability of bacteria embedded within the biofilms. Further, sub MIC of CPCs (0.5 × MIC) significantly reduced the biofilm adhesion (p < 0.001) by representative strains of LM (CCA: 72.2 ± 3.5, CFA: 79.3 ± 0.9, and CSA: 74.9 ± 1.5%) and PA (CCA: 64 ± 1.1, CFA: 67.8 ± 0.8, and CSA: 65.7 ± 4.9%). These results suggested the antibacterial and anti-biofilm potential of CPCs that can be exploited to control foodborne pathogenic infections.

Keywords

Antibiofilm activity Biofilm-forming bacteria Chitosan-phenolic conjugates Foodborne pathogenic bacteria 

Notes

Acknowledgements

The authors would like to thank National Culture Collection for Pathogens, Gyeongsang National University Hospital Branch of (GNUH-NCCP) for supply of pathogens.

Funding

This research was supported by Marine Biotechnology Program funded by Ministry of Oceans and Fisheries, Republic of Korea (Grant number 20150220).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest regarding the publication of this manuscript.

Supplementary material

13205_2018_1451_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 22 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Gabjin Kim
    • 1
  • Chakradhar Dasagrandhi
    • 2
  • Eun-Hye Kang
    • 1
  • Sung-Hwan Eom
    • 3
  • Young-Mog Kim
    • 1
    • 2
  1. 1.Department of Food Science and TechnologyPukyong National UniversityBusanSouth Korea
  2. 2.Marine-Integrated Bionics Research CenterPukyong National UniversityBusanSouth Korea
  3. 3.Department of Food Science and TechnologyDongeui UniversityBusanSouth Korea

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