Evaluation of probiotic Lactobacillus plantarum against foodborne pathogens and its fermentation potential in improving Lolium multiflorum silage quality
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The objective of this study was to isolate the lactic acid bacteria from fermented silage sample and analyze their antibacterial activities, probiotic properties, and fermentation potential in silage. Eleven lactic acid bacteria (LAB) were selected based on distinct morphologies and preliminary studies. Cell-free supernatant (CFS) was then prepared from the selected strains for antibacterial analysis. L-30 strain and its CFS showed highest inhibition (> 10 mm) against tested foodborne pathogens as compared to other strains. Hereafter, the strain L-30 was named as KCC-30 and used for further studies. KCC-30 can survive in the harsh conditions of GIT such as low pH ( 2) and bile salt environment (oxgal) than standard L. plantarum KACC-91016 (pH 2: 27.2% vs 20.5%; oxgal: 72.3% vs 57.7%, both p < 0.05). In addition, KCC-30 exhibited strong auto-aggregation (68.3% vs 51.5%) and co-aggregation (33% vs 23.9%) properties. For silage experiment, KCC-30 treatment did not alter the nutrient profiles of silage. At the same time, KCC-30 treatment increased the lactic acid content of silage as compared to untreated silage (5.55 DM% vs 3.11 DM%). An increase of lactic acid content in the silage is due to higher lactic acid bacteria population in KCC-30 treated silage (15.33 × 107 CFU/g vs 7.66 × 107 CFU/g) than untreated silage (p < 0.05). Overall data suggested that KCC-30 exhibited strong probiotic potential and improved the quality of Lolium multiflorum silage by increasing the lactic acid level. Therefore, KCC-30 could be considered as potential strain to improve the fermentation quality of L. multiflorum silage.
KeywordsLactic acid bacteria Low pH Bile salt Co-aggregation Auto-aggregation Silage fermentation
This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of Quality Improvement and Standardization Technique for Low Moisture Storage Forage, Project no. “PJ010916012017”) funded by Rural Development Administration, Republic of Korea. This study was also supported by 2014 Postdoctoral Fellowship Program of National Institute of Animal Science funded by Rural Development Administration, Republic of Korea.
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Conflict of interest
The authors have no conflicts of interest related to this study to disclose.
- Ali G, Liu Q, Yuan X, Dong Z, Desta ST, Li J, Bai X, Shah AA, Shao T (2017) Characteristics of lactic acid bacteria isolates and their effects on the fermentation quality of acacia (Sophora japonica L.) leaf silage at low temperatures. Grassl Sci 63(3):141–149. https://doi.org/10.1111/grs.12162 CrossRefGoogle Scholar
- Altay F, Karbancıoglu-Güler F, Daskaya-Dikmen C, Heperkan D (2013) A review on traditional Turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics. Int J Food Microbiol 167(1):44–56. https://doi.org/10.1016/j.ijfoodmicro.2013.06.016 CrossRefPubMedGoogle Scholar
- Arasu MV, Jung MW, Ilavenil S, Kim DH, Park HS, Park JW, Al-Dhabi NA, Choi KC (2014b) Characterization, phylogenetic affiliation and probiotic properties of high cell density Lactobacillus strains recovered from silage. J Sci Food Agric 94(12):2429–2440. https://doi.org/10.1002/jsfa.6573 CrossRefPubMedGoogle Scholar
- Bajagai YS, Klieve AV, Dart PJ, Bryden WL (2016) Probiotics in animal nutrition—production, impact and regulation. Food Agric Organ U N 179(1):1–108Google Scholar
- Cezário AS, Ribeiro KG, Santos SA, Valadares Filho SdC, Pereira OG (2015) Silages of Brachiaria brizantha cv. Marandu harvested at two regrowth ages: microbial inoculant responses in silage fermentation, ruminant digestion and beef cattle performance. Anim Feed Sci Technol 208:33–43. https://doi.org/10.1016/j.anifeedsci.2015.06.025.CrossRefGoogle Scholar
- Ferreira DdJ, Lana RdP, Zanine AdM, Santos EM, Veloso CM, Ribeiro GA (2013) Silage fermentation and chemical composition of elephant grass inoculated with rumen strains of Streptococcus bovis. Anim Feed Sci Technol 183(1–2):22–28. https://doi.org/10.1016/j.anifeedsci.2013.04.020 CrossRefGoogle Scholar
- Ilavenil S, Vijayakumar M, Kim daH, Valan Arasu M, Park HS, Ravikumar S, Choi KC (2016) Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass. J Sci Food Agric 96(2):593–601. https://doi.org/10.1002/jsfa.7128 CrossRefPubMedGoogle Scholar
- Rabelo CHS, Basso FC, Lara EC, Jorge LGO, Härter CJ, Mesquita LG, Silva LFP, Reis RA (2018) Effects of Lactobacillus buchneri as a silage inoculant and as a probiotic on feed intake, apparent digestibility and ruminal fermentation and microbiology in wethers fed low-dry-matter whole-crop maize silage. Grass Forage Sci 73(1):67–77. https://doi.org/10.1111/gfs.12303 CrossRefGoogle Scholar
- Tabacco E, Piano S, Cavallarin L, Bernardes TF, Borreani G (2009) Clostridia spore formation during aerobic deterioration of maize and sorghum silages as influenced by Lactobacillus buchneri and Lactobacillus plantarum inoculants. J Appl Microbiol 107(5):1632–1641. https://doi.org/10.1111/j.1365-2672.2009.04344.x CrossRefPubMedGoogle Scholar
- Valan Arasu M, Jung MW, Kim DH, Park HS, Ilavenil S, Al-Dhabi NA, Choon Choi K (2015) Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profiles in grass silage. Ann Microbiol 65(1):15–25. https://doi.org/10.1007/s13213-014-0830-2 CrossRefGoogle Scholar
- Zanine AdM, Bonelli EA, de Souza AL, Ferreira DdJ, Santos EM, Ribeiro MD, Geron LJV, Pinho RMA (2016) Effects of Streptococcus bovis isolated from bovine rumen on the fermentation characteristics and nutritive value of tanzania grass silage. Sci World J 2016:8517698, 8512016. https://doi.org/10.1155/2016/8517698 CrossRefGoogle Scholar