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3 Biotech

, 8:446 | Cite as

Amelioration of arsenic-induced oxidative stress in CHO cells by Ixora coccinea flower extract

  • S. A. Salmataj
  • Shobha U. Kamath
  • V. Ramachandra Murty
  • Sreedhara Ranganath Pai
Original Article

Abstract

Chronic exposure to inorganic arsenic creates various health problems. Ixora coccinea flower extract was investigated for its ability to protect against arsenic-induced cytotoxicity and genotoxicity in CHO cell line. MTT assay confirmed the efficacy of the extract in ameliorating arsenic-induced cytotoxicity. The value (48 mM) of 24 h inhibitory concentration (IC50) of sodium arsenate for CHO cells was obtained by MTT assay. Various free radical scavenging assays like DPPH, ABTS and nitric oxide scavenging assay confirmed antioxidant activity of the Ixora coccinea flower extract. Pretreatment of the extract significantly inhibited the arsenic-induced DNA damage (p < 0.01) in CHO cells. The extract administration significantly (p < 0.01) inhibited the intracellular ROS and depolarization of mitochondrial membrane induced by sodium arsenate. Ixora coccinea flower extract reduced oxidative stress in cells. Antioxidant enzymes like catalase and SOD activity was restored significantly (p < 0.01) in pretreated CHO cells. Ixora coccinea flower extract also exhibited the anti-apoptotic potential by decreasing the percentage apoptotic index (p < 0.01). These results may expand the applications of Ixora coccinea flowers as an alternative food with antioxidant properties and protective functions against arsenic (iAs) induced toxicological effects.

Keywords

Ixora coccinea CHO cells ROS Cytotoxicity Comet assay 

Notes

Acknowledgements

The authors are grateful to Dr. Satish Rao, Professor of Radiobiology division, School of Life Sciences, Manipal Institute of Technology, Manipal College of Pharmaceutical Sciences Manipal Academy of Higher Education for providing laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

References

  1. Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 2nd edn. Verlag Chemie, Weinheim, pp 673–684.  https://doi.org/10.1088/1742-6596/398/1/012032 CrossRefGoogle Scholar
  2. Aung KH, Kurihara R, Nakashima S, Maekawa F, Nohara K, Kobayashi T, Tsukahara S (2013) Inhibition of neurite outgrowth and alteration of cytoskeletal gene expression by sodium arsenite. Neurotoxicology 34:226–235.  https://doi.org/10.1016/j.neuro.2012.09.008 CrossRefPubMedGoogle Scholar
  3. Bai J, Cederbaum AI (2003) Catalase protects HepG2 cells from apoptosis induced by DNA-damaging agents by accelerating the degradation of p53. J Biol Chem 278:4660–4667.  https://doi.org/10.1074/jbc.M206273200 CrossRefPubMedGoogle Scholar
  4. Baliga MS, Kurian PJ (2012) Ixora coccinea Linn.: Traditional uses, phytochemistry and pharmacology. Chin J Integr Med 18:72–79.  https://doi.org/10.1007/s11655-011-0881-3 CrossRefPubMedGoogle Scholar
  5. Bansal P, Paul P, Nayak PG, Pannakal ST, Zou J-H, Laatsch H et al (2011) Phenolic compounds isolated from Pilea microphylla prevent radiation-induced cellular DNA damage. Acta Pharm Sin B 1:226–235.  https://doi.org/10.1016/j.apsb.2011.10.006 CrossRefGoogle Scholar
  6. Bhattacharya S (2017) Medicinal plants and natural products in amelioration of arsenic toxicity: a short review. Pharm Biol 55(1):349–354.  https://doi.org/10.1080/13880209.2016.1235207 CrossRefPubMedGoogle Scholar
  7. Chang C, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182.  https://doi.org/10.1186/s12906-017-1845-6 CrossRefGoogle Scholar
  8. Gouthamchandra K, Mahmood R, Manjunatha H (2010) Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ Toxicol Pharmacol 30:11–18.  https://doi.org/10.1016/j.etap.2010.03.005 CrossRefPubMedGoogle Scholar
  9. John D (1984) One hundred useful raw drugs of Kani tribe of India. Int J Crude Drug Res 22:17–39CrossRefGoogle Scholar
  10. Mazumder DNG (2008) Chronic arsenic toxicity human health. Indian J Med Res 128:436–447.  https://doi.org/10.1016/j.kjms.2011.05.003 CrossRefGoogle Scholar
  11. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63.  https://doi.org/10.1016/0022-1759(83)90303-4 CrossRefPubMedGoogle Scholar
  12. Renvoize C, Biola A, Pallardy M, Breard J (1998) Apoptosis: identification of dying cells. Cell Biol Toxicol 14:111–120.  https://doi.org/10.1023/A:1007429904664 CrossRefPubMedGoogle Scholar
  13. Saha MR, Alam MA, Akter R, Jahangir R (2008) In-vitro free radical scavenging activity of Ixora coccinea L. Bangladesh J Pharmacol 3:90–96.  https://doi.org/10.3329/bjp.v3i2.838 CrossRefGoogle Scholar
  14. Sankaranarayanan S, Bamal P, Ramachandran J, Kalaichelvan PT, Deccaraman M, Vijayalakshimi M et al (2010) Ethnobotanical study of medicinal plants used by traditional users in Villupuram district of Tamilnadu, India. J Med Plants Res 4:1089–1101.  https://doi.org/10.5897/JMPR09.027 CrossRefGoogle Scholar
  15. Scaduto RC Jr, Grotyohann LW (1999) Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J 76:469–477.  https://doi.org/10.1016/S0006-3495(99)77214-0 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191.  https://doi.org/10.1016/0014-4827(88)90265-0 CrossRefPubMedGoogle Scholar
  17. Singh AP, Goel RK, Tajpreet K (2011) Mechanisms pertaining to arsenic toxicity. Toxicol Int 18:87–93.  https://doi.org/10.4103/0971-6580.84258 CrossRefPubMedPubMedCentralGoogle Scholar
  18. Sivaperumal R, Ramya S, Veera Ravi S, Rajasekaran C, Jayakumararaj R (2009) Herbal remedies practiced by Malayali’s to treat skin diseases. Environ Int J Sci Tech 4:35–44Google Scholar
  19. Sivarajan VV, Balachandran I (1994) Ayurvedic drugs and their plant sources. Oxford and IBH Publishing Co. Ltd, New Delhi, p 347Google Scholar
  20. Slinkard J, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28:49–55.  https://doi.org/10.12691/jfnr-3-7-7 CrossRefGoogle Scholar
  21. Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S (2007) Study on antioxidant activity of certain plants in Thailand: mechanism of antioxidant action of guava leaf extract. Food Chem 103:381–388.  https://doi.org/10.1016/j.foodchem.2006.07.034 CrossRefGoogle Scholar
  22. Vadivu N, Jayshree C, Kasthuri K, Rubhini G, Rukmankathan G (2010) Pharmacognostical standardization of leaves of Ixora coccinea Linn. J Pharm Sci Res 2:164–170Google Scholar
  23. Wang Z, Zhao Y, Smith E, Goodall GJ, Drew PA, Brabletz T, Yang C (2011) Reversal and prevention of arsenic induced human bronchial epithelial cell malignant transformation by microRNA—200b. Toxicol Sci 121:110–122.  https://doi.org/10.1093/toxsci/kfr029 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • S. A. Salmataj
    • 1
  • Shobha U. Kamath
    • 2
  • V. Ramachandra Murty
    • 1
  • Sreedhara Ranganath Pai
    • 3
  1. 1.Department of BiotechnologyManipal Institute of Technology, MAHEManipalIndia
  2. 2.Department of BiochemistryKasturba Medical College, Manipal Academy of Higher EducationManipalIndia
  3. 3.Department of PharmacologyManipal College of Pharmaceutical Sciences, MAHEManipalIndia

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