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3 Biotech

, 8:407 | Cite as

Improving palm kernel cake nutrition using enzymatic hydrolysis optimized by Taguchi method

  • Witida Sathitkowitchai
  • Sunee Nitisinprasert
  • Suttipun Keawsompong
Original Article
  • 36 Downloads

Abstract

Enzymatic hydrolysis of palm kernel cake to improve the quality of substrates with multi-response criteria based on the Taguchi orthogonal array. Nine experimental runs were performed based on an L9 orthogonal array. Percent substrate, incubation time, and enzyme units were optimized considering multiple performance characteristics. Analysis of variance was also applied to identify the most significant factors. Results determined percent substrate as the most important factor for enzymatic hydrolysis followed by incubation time and enzyme units. Enzymatic hydrolysis conditions were optimized as percent substrate, incubation time and enzyme units at 14%, 6 h and 750 units, respectively. Tests were conducted to compare experimental and model results. The experimental result (protein release) at optimal condition were three times higher than the predicted mode.

Keywords

Palm kernel cake L9 orthogonal array Enzymatic hydrolysis Taguchi method 

Notes

Acknowledgements

This research was financially supported by a Research and Researchers for Industries (RRI) Ph.D. program (PHD56I0043), The Thailand Research Fund (TRF), Betagro Science Centre Co., Ltd., and Betagro Public Co. Ltd.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest in the publication.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Witida Sathitkowitchai
    • 1
  • Sunee Nitisinprasert
    • 1
    • 2
  • Suttipun Keawsompong
    • 1
    • 2
  1. 1.Department of Biotechnology, Faculty of Agro-IndustryKasetsart UniversityBangkokThailand
  2. 2.Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced StudiesKasetsart University (CASAF, NRU-KU)BangkokThailand

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