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3 Biotech

, 8:398 | Cite as

Bio-production of novel water-soluble yellow pigment from Aspergillus sp. and exploring its sustainable textile applications

  • S. Pandiyarajan
  • P. Premasudha
  • K. Kadirvelu
Original Article
  • 34 Downloads

Abstract

In the present study, 40 pigment-producing microbes were isolated from various soil sources. Among these, a novel water-soluble yellow pigment-producing fungal isolate (MBYP1) was identified as Aspergillus sp. through ITS gene sequencing. The maximum pigment yield (UA430nm, 12.45 ± 0.5 g/l) was obtained when strain MBYP1 was cultured under optimum conditions (28 °C and pH 5.5 under static condition). Subsequently, the pigment was purified through gel chromatography and high-performance liquid chromatography (HPLC). Characterization of purified pigment through UV–Vis and liquid chromatography–mass spectrometry (LC–MS) reveal maximum absorbance at 430 nm and molecular mass of 301 m/z, respectively. Further, the pigment exhibited a maximum dyeing capacity of up to 80% irrespective of mordant. Toxicity evaluation of purified pigment with zebra fish model system reported an IC50 value of 710 µg/mL. Pigment antioxidant ability was established by DPPH (35.7 µg/mL) and phosphomolybdenum assay (226.61 mg/g) thus ascertaining improvised light fastness of dyed fabric. Moreover, lack of antimicrobial activity (up to 40 µg/mL) improves pigment bio-degradability. In collective, the novel yellow pigment from Aspergillus sp. MBYP1 strain was found to be an eco-friendly alternative to synthetic dye for potential applications in textile industries.

Keywords

Yellow pigment Aspergillus sp. Antioxidant Textile dyeing Zebrafish toxicity 

Notes

Acknowledgements

The authors are sincerely grateful to the Defense Research and Development Organization (DRDO), for financial support through Phase II project DRDO-BU CLS, Coimbatore, India. We also acknowledge DFRL, Mysore for great support.

Author contributions

PP and KK designed the experiment. SP performed the experiments, analyzed the data and prepared the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Ahmad WA, Ahmad WY, Zakaria ZA, Yusof NZ (2012) Application of bacterial pigments as colorant. In: Application of bacterial pigments as colorant. Springer, Berlin, pp 57–74CrossRefGoogle Scholar
  2. Akilandeswari P, Pradeep BV (2017) Aspergillus terreus KMBF1501 a potential pigment producer under submerged fermentation. Int J Pharm Pharm Sci 9:38–43CrossRefGoogle Scholar
  3. Bae JT, Sinha J, Park JP, Song CH, Yun JW (2000) Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J Microbiol Biotechnol 10(4):482–487Google Scholar
  4. Balraj J, Pannerselvam K, Jayaraman A (2014) Isolation of pigmented marine bacteria Exiguobacterium sp. from peninsular region of India and a study on biological activity of purified pigment. IJSTR 3(3):375–384Google Scholar
  5. Blanc PJ, Loret MO, Santerre AL, Pareilleux A, Prome D, Prome JC, Laussac JP, Goma G (1994) Pigments of monascus. J Food Sci 59(4):862–865CrossRefGoogle Scholar
  6. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199CrossRefGoogle Scholar
  7. Couto SR (2009) Dye removal by immobilised fungi. Biotechnol Adv 27(3):227–235CrossRefGoogle Scholar
  8. Daroit DJ, Silveira ST, Hertz PF, Brandelli A (2007) Production of extracellular β-glucosidase by Monascus purpureus on different growth substrates. Process Biochem 42(5):904–908CrossRefGoogle Scholar
  9. Dufosse L, Fouillaud M, Caro Y, Mapari SA, Sutthiwong N (2014) Filamentous fungi are large-scale producers of pigments and colorants for the food industry. Curr Opin Biotechnol 26:56–61CrossRefPubMedGoogle Scholar
  10. Dufossé L (2006) Microbial production of food grade pigments. Food Technol Biotechnol 44(3):313–323Google Scholar
  11. Geweely NS (2011) Investigation of the optimum condition and antimicrobial activities of pigments from four potent pigment-producing fungal species. J Life Sci 5(9):697–711Google Scholar
  12. Ghaheh FS, Khoddami A, Alihosseini F, Jing S, Ribeiro A, Cavaco-Paulo A, Silva C (2017) Antioxidant cosmetotextiles: cotton coating with nanoparticles containing vitamin E. Process Biochem 59:46–51CrossRefGoogle Scholar
  13. Gulani C, Bhattacharya S, Das A (2012) Assessment of process parameters influencing the enhanced production of prodigiosin from Serratia marcescens and evaluation of its antimicrobial, antioxidant and dyeing potentials. Malays J Microbiol 8(2):116–122Google Scholar
  14. Gunasekaran S, Poorniammal R (2008) Optimization of fermentation conditions for red pigment production from Penicillium sp. under submerged cultivation. Afr J Biotechnol 7(12):1894–1898CrossRefGoogle Scholar
  15. Indra Arulselvi P, Umamaheswari S, Ranandkumar Sharma G, Karthik C, Jayakrishna C (2014) Screening of yellow pigment producing bacterial isolates from various eco-climatic areas and analysis of the carotenoid produced by the isolate. J Food Process Technol 5(292):2Google Scholar
  16. Jaja-Chimedza A, Sanchez K, Gantar M, Gibbs P, Schmale M, Berry JP (2017) Carotenoid glycosides from cyanobacteria are teratogenic in the zebrafish (Danio rerio) embryo model. Chemosphere 174:478–489CrossRefPubMedPubMedCentralGoogle Scholar
  17. Joshi VK, Attri D, Bala A, Bhushan S (2003) Microbial pigments. Indian J Biotech 2:362–369Google Scholar
  18. Khoo HE, Prasad KN, Kong KW, Jiang Y, Ismail A (2011) Carotenoids and their isomers: color pigments in fruits and vegetables. Molecules 16(2):1710–1738CrossRefPubMedGoogle Scholar
  19. Kohler BE (1995) Electronic structure of carotenoids. ChemInform 26(32):1–12Google Scholar
  20. Kumar A, Vishwakarma HS, Singh J, Dwivedi S, Kumar M (2015) Microbial pigments: production and their applications in various industries. Int J Pharm Chem Biol Sci 5:203–212Google Scholar
  21. Lu M, Zhao C, Zhou P, Yu L (2010) Analysis and identification of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous by high-performance liquid chromatography. Naturforsch C 65(7–8):489–494CrossRefGoogle Scholar
  22. Lu F, Liu L, Huang Y, Zhang X, Wang Z (2018) Production of Monascus pigments as extracellular crystals by cell suspension culture. Appl Microbial biotechnol 102(2):677–687CrossRefGoogle Scholar
  23. Morales-Oyervides L, Oliveira J, Sousa-Gallagher M, Méndez-Zavala A, Montañez JC (2017) Assessment of the dyeing properties of the pigments produced by Talaromyces spp. J Fungi 3(3):38CrossRefGoogle Scholar
  24. O’Neill C, Hawkes FR, Hawkes DL, Lourenço ND, Pinheiro HM, Delée W (1999) Colour in textile effluents–sources, measurement, discharge consents and simulation: a review. J Chem Technol Biotechnol 74(11):1009–1018CrossRefGoogle Scholar
  25. Ogugbue CJ, Sawidis T (2011) Bioremediation and detoxification of synthetic wastewater containing triarylmethane dyes by Aeromonas hydrophila isolated from industrial effluent. Biotechnol Res int 1–11Google Scholar
  26. Prasain JK, Moore R, Hurst JS, Barnes S, van Kuijk FJ (2005) Electrospray tandem mass spectrometric analysis of zeaxanthin and its oxidation products. J Mass spectrom 40(7):916–923CrossRefPubMedGoogle Scholar
  27. Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269(2):337–341CrossRefPubMedPubMedCentralGoogle Scholar
  28. Rajeendran A, Nulit R, Yien CY, Ibrahim MH, Kalhori N (2017) Isolation and molecular identification of Colletotrichum gloeosporioides from infected peanut seeds. IJPSS 19:1–8Google Scholar
  29. Safafar H, Van Wagenen J, Møller P, Jacobsen C (2015) Carotenoids, phenolic compounds and tocopherols contribute to the antioxidative properties of some microalgae species grown on industrial wastewater. Mar Drugs 13(12):7339–7356CrossRefPubMedPubMedCentralGoogle Scholar
  30. Sajilata MG, Singhal RS, Kamat MY (2008) The carotenoid pigment zeaxanthin—a review. Compr Rev Food Sci Food saf 7(1):29–49CrossRefGoogle Scholar
  31. Shahid M, Mohammad F (2013) Recent advancements in natural dye applications: a review. J Clean Prod 53:310–331CrossRefGoogle Scholar
  32. Shen B, Liu HC, Ou WB, Eilers G, Zhou SM, Meng FG, Li CQ, Li YQ (2015) Toxicity induced by basic violet 14, direct red 28 and acid red 26 in zebrafish larvae. J Appl Toxicol 35(12):1473–1480CrossRefPubMedGoogle Scholar
  33. Singh R, Jain A, Panwar S, Gupta D, Khare SK (2005) Antimicrobial activity of some natural dyes. Dyes Pigments 66(2):99–102CrossRefGoogle Scholar
  34. Singh D, Puri M, Wilkens S, Mathur AS, Tuli DK, Barrow CJ (2013) Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand. Mar Bioresour Technol 143:308–314CrossRefGoogle Scholar
  35. Thiagarajan P, Nalankilli G (2013) Improving light fastness of reactive dyed cotton fabric with antioxidant and UV absorbers. Indian J Fiber Text Res 48:161–164Google Scholar
  36. Tuli HS, Chaudhary P, Beniwal V, Sharma AK (2015) Microbial pigments as natural color sources: current trends and future perspectives. J Food Sci Technol 52(8):4669–4678CrossRefPubMedGoogle Scholar
  37. Venil CK, Aruldass CA, Dufossé L, Zakaria ZA, Ahmad WA (2014) Current perspective on bacterial pigments: emerging sustainable compounds with coloring and biological properties for the industry—an incisive evaluation. RSC Adv 4(74):39523–39529CrossRefGoogle Scholar
  38. Walton K, Gantar M, Gibbs PD, Schmale MC, Berry JP (2014) Indole alkaloids from Fischerella inhibit vertebrate development in the zebrafish (Danio rerio) embryo model. Toxins 6(12):3568–3581CrossRefPubMedPubMedCentralGoogle Scholar
  39. Young AJ, Lowe GL (2018) Carotenoids—antioxidant properties. Antioxidants 7:28–31CrossRefPubMedCentralGoogle Scholar
  40. Zhong K, Gao XL, Xu ZJ, Gao H, Fan S, Yamaguchi I, Li LH, Chen RJ (2011) Antioxidant activity of a novel Streptomyces strain Eri 12 isolated from the Rhizosphere of Rhizoma curcumae longae. Curr Res Bacteriol 4(2):63–72CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.DRDO-BU CLS, Bharathiar University CampusCoimbatoreIndia
  2. 2.Department of Nanoscience and TechnologyBharathiar UniversityCoimbatoreIndia

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