3 Biotech

, 8:398 | Cite as

Bio-production of novel water-soluble yellow pigment from Aspergillus sp. and exploring its sustainable textile applications

  • S. Pandiyarajan
  • P. PremasudhaEmail author
  • K. Kadirvelu
Original Article


In the present study, 40 pigment-producing microbes were isolated from various soil sources. Among these, a novel water-soluble yellow pigment-producing fungal isolate (MBYP1) was identified as Aspergillus sp. through ITS gene sequencing. The maximum pigment yield (UA430nm, 12.45 ± 0.5 g/l) was obtained when strain MBYP1 was cultured under optimum conditions (28 °C and pH 5.5 under static condition). Subsequently, the pigment was purified through gel chromatography and high-performance liquid chromatography (HPLC). Characterization of purified pigment through UV–Vis and liquid chromatography–mass spectrometry (LC–MS) reveal maximum absorbance at 430 nm and molecular mass of 301 m/z, respectively. Further, the pigment exhibited a maximum dyeing capacity of up to 80% irrespective of mordant. Toxicity evaluation of purified pigment with zebra fish model system reported an IC50 value of 710 µg/mL. Pigment antioxidant ability was established by DPPH (35.7 µg/mL) and phosphomolybdenum assay (226.61 mg/g) thus ascertaining improvised light fastness of dyed fabric. Moreover, lack of antimicrobial activity (up to 40 µg/mL) improves pigment bio-degradability. In collective, the novel yellow pigment from Aspergillus sp. MBYP1 strain was found to be an eco-friendly alternative to synthetic dye for potential applications in textile industries.


Yellow pigment Aspergillus sp. Antioxidant Textile dyeing Zebrafish toxicity 



The authors are sincerely grateful to the Defense Research and Development Organization (DRDO), for financial support through Phase II project DRDO-BU CLS, Coimbatore, India. We also acknowledge DFRL, Mysore for great support.

Author contributions

PP and KK designed the experiment. SP performed the experiments, analyzed the data and prepared the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.DRDO-BU CLS, Bharathiar University CampusCoimbatoreIndia
  2. 2.Department of Nanoscience and TechnologyBharathiar UniversityCoimbatoreIndia

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