Bio-production of novel water-soluble yellow pigment from Aspergillus sp. and exploring its sustainable textile applications
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In the present study, 40 pigment-producing microbes were isolated from various soil sources. Among these, a novel water-soluble yellow pigment-producing fungal isolate (MBYP1) was identified as Aspergillus sp. through ITS gene sequencing. The maximum pigment yield (UA430nm, 12.45 ± 0.5 g/l) was obtained when strain MBYP1 was cultured under optimum conditions (28 °C and pH 5.5 under static condition). Subsequently, the pigment was purified through gel chromatography and high-performance liquid chromatography (HPLC). Characterization of purified pigment through UV–Vis and liquid chromatography–mass spectrometry (LC–MS) reveal maximum absorbance at 430 nm and molecular mass of 301 m/z, respectively. Further, the pigment exhibited a maximum dyeing capacity of up to 80% irrespective of mordant. Toxicity evaluation of purified pigment with zebra fish model system reported an IC50 value of 710 µg/mL. Pigment antioxidant ability was established by DPPH (35.7 µg/mL) and phosphomolybdenum assay (226.61 mg/g) thus ascertaining improvised light fastness of dyed fabric. Moreover, lack of antimicrobial activity (up to 40 µg/mL) improves pigment bio-degradability. In collective, the novel yellow pigment from Aspergillus sp. MBYP1 strain was found to be an eco-friendly alternative to synthetic dye for potential applications in textile industries.
KeywordsYellow pigment Aspergillus sp. Antioxidant Textile dyeing Zebrafish toxicity
The authors are sincerely grateful to the Defense Research and Development Organization (DRDO), for financial support through Phase II project DRDO-BU CLS, Coimbatore, India. We also acknowledge DFRL, Mysore for great support.
PP and KK designed the experiment. SP performed the experiments, analyzed the data and prepared the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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