3 Biotech

, 8:387 | Cite as

Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213

  • Jinlong Li
  • Weijia Shen
  • Guangsen Fan
  • Xiuting LiEmail author
Original Article


A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of this lipase was pH 8.0 and 50 °C, respectively. The lipase retained > 85% activity in isopropanol and acetone at 30 °C for 10 min but the activity was reduced to 10.6% in n-hexane. Mg2+, Al3+, Mn2+, and Fe3+ enhanced lipase activity at both 1 mM and 5 mM concentrations. p-NPP, a long-chain acyl group 4-NP ester, appeared to be a good substrate candidate.


B. pyrrocinia Lipase Screening Purification Characterization 



This work was supported by the National Natural Science Foundation of China (Grant Numbers: 31501487, 31671798). We are grateful to Professor Madhav P. Yadav for his helpful promoting in the language quality of this article.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

13205_2018_1414_MOESM1_ESM.docx (1018 kb)
Supplementary material 1 (DOCX 1018 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human HealthBeijing Technology and Business UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Higher Institution Engineering Research Center of Food Additives and IngredientsBeijingPeople’s Republic of China
  3. 3.Beijing Key Laboratory of Flavor ChemistryBeijingPeople’s Republic of China

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