3 Biotech

, 8:379 | Cite as

Characterization of novel LMW glutenin subunit genes at the Glu-M3 locus from Aegilops comosa

  • Lin Huang
  • Yu He
  • Yarong Jin
  • Fang Wang
  • Jingshu He
  • Lihua Feng
  • Dengcai Liu
  • Bihua WuEmail author
Original Article


We report the characterization of seven novel low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops comosa (2n = 2x = 14, MM). We found that all seven LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Three genes (comosa-M1, comosa-M2, and comosa-M3) encode LMW-m-type subunits, two (comosa-I1 and comosa-I2) encode LWM-i-type subunits, and two (comosa-L1 and comosa-L2) encode LWM-l-type subunits. The comosa-M1 possessed seven cysteine residues, which resulted from a single-nucleotide polymorphism (SNP) of the G-A transition in the fifth position of the N-terminal sequence. Two l-type subunits, comosa-L1 and comosa-L2, contained nine cysteine residues with an extra cysteine residue located in the signal peptide and repetitive domain. In addition, a long insertion of 13 residues (LGQQPQ8/LVQQPQ8) was present in the end of the C-terminal II. Phylogenetic analysis implied that the comosa-L2 may be the intermediate type during the evolution of LMW-l and LMW-i-type genes. Our results demonstrated that the novel LMW-GSs, such as comosa-M1, comosa-L1, and comosa-L2, may have positive effects on dough properties.


Aegilops comosa LMW-GS M genome Wheat quality improvement 



This work was financially supported by grants from the National Natural Science Foundation of China (no. 31571668), the National Key Research and Development Program (no. 2017YFD0100900), and the Education Department of Sichuan Province (no. 18ZA0392).

Compliance with ethical standards

Conflict of interest

The authors state that there is no conflict of interest.


  1. Anjum FM, Khan MR, Din A, Saeed M, Pasha I, Arshad MU (2007) Wheat gluten: high molecular weight glutenin subunits-structure, genetics, and relation to dough elasticity. J Food Sci 72:R56–R63CrossRefPubMedGoogle Scholar
  2. Cassidy BG, Dvorak J, Anderson OD (1998) The wheat low molecular weight glutenin genes: characterization of six new genes and progress in understanding gene family structure. Theor Appl Genet 96:743–750CrossRefGoogle Scholar
  3. Cloutier S, Rampitsch C, Penner G, Lukow O (2001) Cloning and expression of a LMW-i glutenin gene. J Cereal Sci 33:143–154CrossRefGoogle Scholar
  4. D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339CrossRefGoogle Scholar
  5. Dong LL, Zhang XF, Liu DC, Fan HJ, Sun JZ, Zhang ZJ, Qin HJ, Li B, Hao ST, Li ZS, Wang DW, Zhang AM, Ling HQ (2010) New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS One 5:e13548CrossRefPubMedPubMedCentralGoogle Scholar
  6. Hall T (2007) BioEdit, version 7.0.9. Computer program and documentation. lbis Biosciences, CarlsbadGoogle Scholar
  7. Harberd NP, Bartels D, Thompson RD (1985) Analysis of the gliadin multigene loci in bread wheat using nullisomic-tetrasomic lines. Mol Gen Genet 198:234–242CrossRefGoogle Scholar
  8. Henkrar F, El-Haddoury J, Iraqi D, Bendaou N, Udupa SM (2017) Allelic variation at high-molecular weight and low-molecular weight glutenin subunit genes in Moroccan bread wheat and durum wheat cultivars. 3 Biotech 7:287CrossRefPubMedPubMedCentralGoogle Scholar
  9. Ikeda TM, Nagamine T, Fukuoka H, Yano H (2002) Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104:680–687CrossRefPubMedGoogle Scholar
  10. Jiang C, Pei Y, Zhang Y, Li X, Yao D, Yan Y, Ma W, Hsam SL, Zeller FJ (2008) Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi. Hereditas 145:92–98CrossRefPubMedGoogle Scholar
  11. Johal J, Gianibelli MC, Rahman S, Morell MK, Gale KR (2004) Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. Theor Appl Genet 109(5):1028–1040CrossRefPubMedGoogle Scholar
  12. Lan QX, Feng B, Xu ZB, Zhao GJ, Wang T (2013) Molecular cloning and characterization of five novel low molecular weight glutenin subunit genes from Tibetan wheat landraces (Triticum aestivum L.). Genet Resour Crop Ev 60:799–806CrossRefGoogle Scholar
  13. Lee JY, Beom HR, Altenbach SB, Lim SH, Kim YT, Kang CS, Yoon UH, Gupta R, Kim ST, Ahn SN, Kim YM (2016) Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Funct Integr Genom 16:269–279CrossRefGoogle Scholar
  14. Li XH, Wang AL, Xiao YH, Yan YM, He ZH, Appels R, Ma WJ, Hsam SLK, Zeller FJ (2008) Cloning and characterization of a novel low molecular weight glutenin subunit gene at the Glu-A3 locus from wild emmer wheat (Triticum turgidum L. var. dicoccoides). Euphytica 159:181–190CrossRefGoogle Scholar
  15. Li XJ, Liu TH, Song LJ, Zhang H, Li LQ, Gao X (2016) Influence of high-molecular-weight glutenin subunit composition at Glu-A1 and Glu-D1 loci on secondary and micro structures of gluten in wheat (Triticum aestivum L.). Food Chem 213:728–734CrossRefPubMedGoogle Scholar
  16. Luo Z, Chen F, Feng D, Xia G (2005) LMW-GS genes in Agropyron elongatum and their potential value in wheat breeding. Theor Appl Genet 111:272–280CrossRefPubMedGoogle Scholar
  17. Luo G, Zhang X, Zhang Y, Yang W, Li Y, Sun J, Zhan K, Zhang A, Liu D (2015) Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biol 15:68CrossRefPubMedPubMedCentralGoogle Scholar
  18. Ma W, Appels R, Bekes F, Larroque O, Morell MK, Gale KR (2005) Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theor Appl Genet 111:410–422CrossRefPubMedGoogle Scholar
  19. Qin L, Liang Y, Yang D, Sun L, Xia G, Liu S (2015) Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution. Dev Genes Evol 225:31–37CrossRefPubMedGoogle Scholar
  20. Riley R, Chapman V, Johnson R (1968) Introduction of yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217:383–384CrossRefGoogle Scholar
  21. Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76CrossRefPubMedGoogle Scholar
  22. Sabelli PA, Shewry PR (1991) Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis. Theor Appl Genet 83:209–216CrossRefPubMedGoogle Scholar
  23. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
  24. Shewry PR, Tatham AS (1997) Disulphide bonds in wheat gluten proteins. J Cereal Sci 25:207–227CrossRefGoogle Scholar
  25. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  26. Tanaka H, Shimizu R, Tsujimoto H (2005) Genetical analysis of contribution of low-molecular-weight glutenin subunits to dough strength in common wheat (Triticum aestivum L.). Euphytica 141:157–162CrossRefGoogle Scholar
  27. Wang K, Gao L, Wang S, Zhang Y, Li X, Zhang M, Xie Z, Yan Y, Belgard M, Ma W (2011) Phylogenetic relationship of a new class of LMW-GS genes in the M genome of Aegilops comosa. Theor Appl Genet 122:1411–1425CrossRefPubMedGoogle Scholar
  28. Zhao H, Wang R, Guo A, Hu S, Sun G (2004) Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in bread wheat. Hereditas 141:193–198CrossRefPubMedGoogle Scholar
  29. Zhao XL, Xia XC, He ZH, Gale KR, Lei ZS, Appels R, Ma W (2006) Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat. Theor Appl Genet 113:1247–1259CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityWenjiangChina

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