3 Biotech

, 8:372 | Cite as

Optimization of the enzymatic hydrolysis of rice protein by different enzymes using the response surface methodology

  • Jessika Gonçalves dos Santos AguilarEmail author
  • Ruann Janser Soares de Castro
  • Helia Harumi Sato
Original Article


The optimization of the enzymatic hydrolysis of rice protein was determined using an experimental design tool. The semi-purified protease of Bacillus licheniformis LBA 46 and commercial protease Alcalase 2.4 L were used to produce rice hydrolysates using pH values ranging from 6 to 10 and enzyme concentrations varying from 50 to 150 U/mL. The optimized conditions were validated, and using the chosen conditions (pH 10 and 100 U/mL of protease), it was possible to confirm that the model was predictive for oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) responses. The experimental values for the ORAC and FRAP responses were 940 and 18.78 TE µmol/g for the rice protein hydrolysates prepared with LBA protease and 1001.94 and 19.31 TE µmol/g for the rice protein hydrolysates prepared with Alcalase 2.4 L. After optimization of the enzymatic hydrolysis conditions, the antioxidant activity values increased when compared to the values for the intact rice protein: 324.97 TE µmol/g (ORAC) and 6.14 TE µmol/g (FRAP). It was also observed that the LBA protease had an action similar to the commercial protease, showing its potential for application in protein hydrolysis.


Optimization Contour curve Rice Antioxidant Protease 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13205_2018_1401_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 21 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Food Science, School of Food EngineeringUniversity of CampinasCampinasBrazil

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