Degradation of cinnamic acid by a newly isolated bacterium Stenotrophomonas sp. TRMK2
- 25 Downloads
A bacterium Stenotrophomonas sp. TRMK2 capable of utilizing cinnamic acid was isolated from agro-industrial waste by enrichment culture technique. This strain completely utilizes 5 mM cinnamic acid within 18 h of incubation. The different metabolites formed during the degradation of cinnamic acid were characterized by GC-HRMS. The involvement of various enzymes, namely cinnamate reductase, 3-phenylpropionic acid hydroxylase, p-hydroxybenzoic acid hydroxylase and protocatechuate 3,4-dioxygenase in cinnamic acid degradation was demonstrated. A catabolic pathway for cinnamic acid in Stenotrophomonas sp. TRMK2 is as follows: Cinnamic acid; 3-Phenylpropionic acid; 3-(4-Hydroxyphenyl) propionic acid; 4-Hydroxy benzoic acid and Protocatechuic acid. Further, this strain is capable of utilizing various phenolic compounds.
KeywordsBiodegradation Enzymes Metabolism Pollutants Waste
One of the authors Mr. Monisha T. R. would like to thank University Grants Commission (UGC), New Delhi, India for the financial assistance in the mode of BSR Fellowship. Dr. T. B Karegoudar is grateful to UGC for awarding UGC-BSR faculty fellowship. The financial support from UGC in the form of UGC-SAP programme [No.F.4–27/2015/DRS-II (SAP-II)] sanctioned to the department is highly acknowledged.
Compliance with ethical standards
Conflict of interest
Authors declare that the manuscript does not contain any conflict of interest.
- Coulson CB, Evans WC (1959) Microbiological degradation of trans-cinnamic acid by soil Pseudomonas. Chem Ind 17:543–544Google Scholar
- Díaz E, Ferrández A, Prieto MA, José LG (2001) Biodegradation of Aromatic compounds by Escherichia coli. Appl Environ Microbiol 65:523–569Google Scholar
- Hartley RD, Whitehead DC (1985) Phenolic acids in soils and their influence on plant growth and soil microbial processes. In: Vaughan D, Malcolm RE (eds) Soil organic matter and biological activity, developments in plant and soil sciences series, 16. Kluwer Academic Publishers, DordrechtGoogle Scholar
- Kawakami H (1980) Degradation of lignin-related compounds by several Pseudomonads. In: Kirk TK, Higuchi T, Chang HM (eds) Lignin biodegradation: microbiology, chemistry, and potential applications, 2. CRC Press, Inc., Boca Raton, pp 103–125Google Scholar
- Lane DJ (1991) Small subunit ribosomal RNA sequences and primers. Large subunit ribosomal RNA sequences and primers. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics, Wiley, Chichester, pp 148–175Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275Google Scholar
- Singh NB, Yadav K, Amist N (2013) Phytotoxic effects of cinnamic acid on cabbage (Brassica oleracea var. capitata). J Stress Physiol Biochem 92:307–317Google Scholar
- Subba Rao PV, Nambudiri AMD, Bhat JV (1971) Microbial degradation of phenylpropionoids compounds. J Sci Ind Res 30:663–679Google Scholar