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3 Biotech

, 8:381 | Cite as

De novo transcriptome analysis deciphered polyoxypregnane glycoside biosynthesis pathway in Gymnema sylvestre

  • Kuldeepsingh A. Kalariya
  • Dipal B. Minipara
  • Ponnuchamy Manivel
Original Article

Abstract

Gymnema sylvestre is an important medicinal plant containing antidiabetic activity. Through de novo transcriptomic study, the pathways of polyoxypregnane glycosides were explored and candidate genes of these pathways were identified in G. sylvestre. High-quality raw reads were assembled into transcripts which resulted in 193,615 unigenes. These unigenes further decoded 58,274 coding DNA sequences (CDSs). Functional annotation of predicted CDSs was carried out using the protein databases, i.e., NCBI’s non-redundant, Uniprot and Pfam. Eukaryotic orthologous group (KOG) classification and transcription factor analysis has revealed most CDS-enriched categories as “Signal transduction mechanism” and “Basic Helix loop helix” (bHLH) transcription factor family, respectively. A total of 16,569 CDSs were assigned minimum one Gene Ontology (GO) term. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis disclosed 235 CDSs which represented total 27 genes of pregnane glycoside pathways and 19 CDSs represented 10 important enzymes of polyoxypregnane glycoside biosynthesis, i.e., sterol 24-C-methyltransferase, cycloeucalenol cycloisomerase, Δ14-sterol reductase, C-8,7 sterol isomerase, sterol methyltransferase 2, C-5 sterol desaturase, sterol Δ7 reductase, Δ24 sterol reductase, 3β-hydroxysteroid dehydrogenase and progesterone 5β reductase (5βPOR). This transcriptome analysis provided an important resource for future functional genomic studies in G. sylvestre.

Keywords

Gymnema sylvestre Next generation sequencing Pregnane glycosides RNA sequencing 

Notes

Acknowledgements

We acknowledge the funding through FAP Scheme 2015–16, sanctioned dated 23.09.2016 from GSBTM, Govt. of Gujarat, Gujarat, India, the ICAR-DMAPR, Anand and the ICAR, New Delhi for providing the basic facilities for this research work, all germplasm explorers who collected this germplasm and all curators who maintained this genotype at our research farm.

Author contributions

KK conceived the project, designed the experiment, analyzed the data and drafted the MS. DM analyzed the data and drafted the MS. PM explored the germplasm used, gave overall technical and administrative support in this study.

Compliance with ethical standards

Availability of data and materials

The data generated or analyzed during this study are included in this published article, its supplementary information files, and publicly available repositories. The transcriptome raw data are deposited at NCBI under submission ID: SUB2977090, sample ID: GSLFDGS03 and SRA accession ID: SRR5965322.

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Kuldeepsingh A. Kalariya
    • 1
  • Dipal B. Minipara
    • 1
  • Ponnuchamy Manivel
    • 1
  1. 1.ICAR-Directorate of Medicinal and Aromatic Plants ResearchAnandIndia

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