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3 Biotech

, 8:367 | Cite as

Augmentation of pyrethrins content in callus of Chrysanthemum cinerariaefolium and establishing its insecticidal activity by molecular docking of NavMS Sodium Channel Pore receptor

  • M. Paramesha
  • S. Manivannan
  • S. J. Aditya Rao
  • K. S. Srikanth
  • Bhagyalakshmi Neelwarne
  • Nandini P. Shetty
Original Article

Abstract

Pyrethrins are effective food-grade bio-pesticides obtained from the flowers of Chrysanthemum cinerariaefolium and this crop cannot be cultivated widely in India due to its specific agro-climatic requirement. Hence pyrethrins are mostly imported from Kenya. Therefore, the present study aims to develop a process for augmentation of pyrethrin contents in C. cinerariaefolium callus and establish the correlation between early knockdown effects through docking on grain storage insect. In vitro seedlings were used as explants to induce callus on MS medium with different concentrations of auxins and cytokinins. Pyrethrin extracted from the callus was estimated by RP-HPLC. In callus, total pyrethrin was found to be 17.5 µg/g, which is higher than that found in natural flowers of certain Pyrethrum cultivars. The concentrations of cinerin II, pyrethrin II and jasmoline II were quite high in callus grown on solid medium. Bio-efficacy of pyrethrum extracts of flower and callus on insect Tribolium sp., showed higher repellency and early knock-down effect when compared with pure compound pestanal. Further, the rapid knockdown effect of all pyrethrins components was established by molecular docking studies targeting NavMS Sodium Channel Pore receptor docking followed by multiple ligands simultaneous docking, performed to investigate the concurrent binding of different combinations of pyrethrin. Among the six pyrethrin components, the pyrethrin I and II were found to be a more efficient, binding more firmly to the target, exhibiting higher possibilities of insecticidal effect by an early knockdown mechanism.

Keywords

Tribolium sp. C. cinerariaefolium Sodium-gate receptor Bio-pesticides Molecular docking 

Notes

Acknowledgements

The work was funded by CSIR, New Delhi (Network project BSC-0105) and authors express their gratitude to the Director, CSIR-Central Food Technological Research Institute, Mysore, for his support.

Author contributions

NPS, BLN and PM designed the research. PM, SKS and MS performed the experiments and ARSJ and PM performed in silico analysis. PM wrote the paper. All authors approved the manuscript.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest from the funding sources or any authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • M. Paramesha
    • 1
  • S. Manivannan
    • 2
  • S. J. Aditya Rao
    • 3
  • K. S. Srikanth
    • 1
  • Bhagyalakshmi Neelwarne
    • 1
  • Nandini P. Shetty
    • 1
  1. 1.Plant Cell Biotechnology DepartmentCSIR-Central Food Technological Research InstituteMysoreIndia
  2. 2.Food Protectants and Infestation Control DepartmentCSIR-Central Food Technological Research InstituteMysoreIndia
  3. 3.Molecular Biomedicine Laboratory, Department of BiotechnologySahyadri Science College, Kuvempu UniversityShivmogaIndia

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