3 Biotech

, 8:356 | Cite as

Influence of media supplements on inhibition of oxidative browning and bacterial endophytes of Camellia sinensis var. sinensis

  • Karthikeyan Alagarsamy
  • Lubobi Ferdinand Shamala
  • Shu WeiEmail author
Protocols and Methods


Explant oxidative browning and necrosis of Camellia sinensis var. sinensis is a severe problem in tissue culture, often associated with the exuded phenolic compounds and microbial contamination from the explants. In this study, 2-aminoindane-2-phosphonic acid (AIP), an inhibitor of the polyphenol production-required enzyme phenylalanine ammonia lyase (PAL), and different antibiotics were tested to control tea explant necrosis and browning. These compounds were supplemented in the regular plant growth medium together with 6-benzylaminopurine and thidiazuron at different concentrations. Our data indicated that application of 2 µM of AIP was able to effectively inhibit callus browning, significantly reduce EGC abundance, and greatly improve callus induction and growth. Moreover, the use of 150 mg/L of timentin and 30 mg/L gentamycin resulted in an effective elimination of the surface and endophytic microbes associated with explants of C. sinensis var. sinensis. Our study revealed that the inhibition of PAL using AIP combined with the two tested antibiotics could open up new doors to control oxidative tissue browning and endophyte contamination in tissue culture for tea genetic manipulation.


2-Aminoindane-2-phosphonic acid (AIP) Bacterial endophytes Phenolic compounds Phenylalanine ammonia lyase (PAL) 



The authors thank the National Natural Science Foundation of China (Grant numbers 31070614 and 31370687) and the Research Fund for the Doctoral Program of Higher Education (20123418110002).

Author contributions

KA and LFS conducted the experiments and prepared the manuscript; SW provided critical suggestions over the study and finalized the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefeiChina

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