3 Biotech

, 8:209 | Cite as

Biochemical events during somatic embryogenesis in Coffea arabica L.

  • Patrícia Monah Cunha Bartos
  • Hugo Teixeira Gomes
  • Lourdes Isabel Velho do Amaral
  • João Batista Teixeira
  • Jonny Everson Scherwinski-Pereira
Original Article
  • 13 Downloads

Abstract

Several biochemical components associated with different stages of somatic embryogenesis in coffee (Coffea arabica L.) are investigated using foliar explants. Soluble sugar, starch, free amino acids and total proteins were extracted and quantified at different stages of somatic embryogenesis, such as foliar segments (initial explants), primary calluses, embryogenic calluses, globular embryos, torpedoes, cotyledonary embryos and mature fruit zygotic embryos. Total soluble sugar levels increased sixfold at the initial stages of somatic embryogenesis induction. During this period, total soluble sugar in the cultures contained approximately 99.3% glucose and fructose. At 67.4 μg/mg MS, no significant changes were observed in total sugar content during the embryo’s somatic maturation and regeneration. During this stage, total soluble sugar was composed of 60% sucrose. After primary callus formation, starch contents increased gradually until the culture’s conclusion. Total free amino acids, particularly arginine, lysine, methionine, asparagine, glutamine and histidine, revealed a higher synthesis until the formation of the primary callus, after which they remain statistically constant up to the end of the process. During the induction of calluses, a gradual increase of total proteins occurred, which, in the differentiating and maturing of somatic embryos, did not differ statistically till the formation of a cotyledonary embryo, when rates decreased 21.8%.

Keywords

Rubiaceae Morphogenesis Somatic embryos Liquid medium Large-scale propagation Biochemical profiling 

Notes

Acknowledgements

The authors are very grateful to CAPES and CNPq for financial support and fellowships.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BotanyUniversity of BrasíliaBrasíliaBrazil
  2. 2.Embrapa Genetic Resources and BiotechnologyBrasíliaBrazil

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