3 Biotech

, 8:180 | Cite as

Effect of cytokinins on in vitro multiplication, volatiles composition and rosmarinic acid content of Thymus leucotrichus Hal. shoots

  • Tuba Bekircan
  • Ahmet Yaşar
  • Sercan Yıldırım
  • Münevver Sökmen
  • Atalay Sökmen
Original Article
  • 13 Downloads

Abstract

An efficient in vitro multiplication protocol was designed to Thymus leucotrichus, a subshrub and perennial herb growing naturally in the Northwest of Turkey. Of all basal media studied, Murashige and Skoog medium was found to be superior to the others, providing higher shoot formation and the maximum shoot length. Varying concentrations of cytokinins, i.e., 6-benzyladenine, thidiazuron, 2-isopentenyladenine and kinetin were supplemented in the nutrient media to observe their effects on shoot development and biomass. Rosmarinic acid content and volatile compositions of both naturally growing plants and in vitro multiplied plantlets were also evaluated. 6-benzyladenine (1.0 mg/L) and kinetin (0.5 mg/L) were found to be optimum for shoot number and shoot elongation, respectively. Thidiazuron (1.0 mg/L) was superior for biomass production. Rosmarinic acid content of in vitro multiplied plants was found to be higher than that of wild plants, reaching a maximum with 0.5 mg/L 2-isopentenyladenine, which yielded 10.15 mg/g dry weight. The highest thymol content was obtained with 1.0 mg/L kinetin (55.82%), while thidiazuron (0.1 mg/L) increased carvacrol production (12.53%). Overall, Murashige and Skoog medium supplemented with 1.0 mg/L kinetin was determined to be the most favorable medium studied.

Keywords

Biomass Cytokinins Volatiles composition Multiplication Rosmarinic acid Thymus leucotrichus 

Notes

Acknowledgements

The authors thank King Saud University’s Distinguished Scientist Fellowship Program (DSFP) for financial support.

Compliance with ethical standards

Conflict of interest

Authors declare that there is no conflict of interest.

References

  1. Adams RP (2004) Identification of essential oil components by gas chromatography–mass spectroscopy. Allured, Carol StreamGoogle Scholar
  2. Affonso VR, Bızzo HR, Lage CLS, Sato A (2009) Influence of growth regulators in biomass production and volatile profile of in vitro plantlets of Thymus vulgaris L. J Agric Food Chem 57:6392–6395CrossRefGoogle Scholar
  3. Bakhtiar Z, Mirjalili MH, Sonboli A, Farimani MM, Ayyari M (2014) In vitro propagation, genetic and phytochemical assessment of Thymus persicus—a medicinally important source of pentacyclic triterpenoids. Biologia 69:594–603CrossRefGoogle Scholar
  4. Bernard F, Moghadam NN, Mirzajani F (2015) The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines. In Vitro Cell Dev Biol Plant 51:546–553CrossRefGoogle Scholar
  5. Coelho N, Gonçalves S, Gonzalez-Benito ME, Romano A (2012) Establishment of an in vitro propagation protocol for Thymus lotocephalus, a rare aromatic species of the Algarve (Portugal). Plant Growth Regul 66:69–74CrossRefGoogle Scholar
  6. Costa P, Gonçalves S, Valentão P, Andrade PB, Coelho N, Romano A (2012) Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity. Food Chem 135:1253–1260CrossRefGoogle Scholar
  7. Costa P, Gonçalves S, Valentão P, Andrade PB, Romano A (2013) Accumulation of phenolic compounds in in vitro cultures and wild plants of Lavandula viridis L’Hér and their antioxidant and anti-cholinesterase potential. Food Chem Toxicol 57:69–74CrossRefGoogle Scholar
  8. Davis PH (1988) Flora of Turkey and the East Aegean Islands, Supplement 1. University Press, EdinburghGoogle Scholar
  9. Döring AS, Pellegrini E, Della Batola M, Nali C, Lorenzini G, Petersen M (2014) How do background ozone concentrations affect the biosynthesis of rosmarinic acid in Melissa officinalis? J Plant Physiol 171:35–41CrossRefGoogle Scholar
  10. Furmanowa M, Olszowska O (1992) Micropropagation of Thyme. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 19. Springer, Berlin, pp 230–243Google Scholar
  11. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50:151–158CrossRefGoogle Scholar
  12. Hassannejad S, Bernard F, Mirzajani F, Gholami M (2012) SA improvement of hyperhydricity reversion in Thymus daenensis shoots culture may be associated with polyamines changes. Plant Physiol Biochem 51:40–46CrossRefGoogle Scholar
  13. Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hotric Amstedam 108:105–120CrossRefGoogle Scholar
  14. Kikowska M, Thiem B, Sliwinska E, Rewers M, Kowalczyk M, Stochmal A, Oleszek W (2014) The effect of nutritional factors and plant growth regulators on micropropagation and production of phenolic acids and saponins from plantlets and adventitious root cultures of Eryngium maritimum L. J Plant Growth Regul 33:809–819CrossRefGoogle Scholar
  15. Könemann T (1999) Botanica. Gordon Cheers Publication, Hong Kong, p 1020Google Scholar
  16. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127CrossRefGoogle Scholar
  17. Marco-Medina A, Casas JL (2015) In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant. Plant Cell Tissue Organ Cult 120:99–108CrossRefGoogle Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  19. National Institute of Standards and Technology (2008) NIST\EPA 365 \NIH Mass Spectral Library, Version 2.0Google Scholar
  20. Ozdemir E, Alpınar K (2015) An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde-Turkey). J Ethnopharmacol 166:53–65CrossRefGoogle Scholar
  21. Ozudogru EA, Kaya E, Kirdok E, Issever-Ozturk S (2011) In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cell Dev Biol Plant 47:309–320CrossRefGoogle Scholar
  22. Polat R, Cakilcioglu U, Kaltalioglu K, Ulusan MD, Türkmen Z (2015) An ethnobotanical study on medicinal plants in Espiye and its surrounding (Giresun-Turkey). J Ethnopharmacol 163:1–11CrossRefGoogle Scholar
  23. Razzaque A, Ellis BE (1977) Rosmarinic acid production in Coleus cell cultures. Planta 137:287–291CrossRefGoogle Scholar
  24. Sáez F, Sánchez P, Piqueras A (1994) Micropropagation of Thymus piperella. Plant Cell Tissue Organ Cult 39:269–272CrossRefGoogle Scholar
  25. Santos-Gomes PC, Seabra RM, Andrade PB, Fernandes-Ferreira M (2003) Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalis L.). J Plant Physiol 160:1025–1032CrossRefGoogle Scholar
  26. Sariboga B, Korkmaz H (2009) Antimicrobial activity of Thymus leucotrichus and Origanum laevigatum. Asian J Chem 21:2777–2781Google Scholar
  27. Shenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204CrossRefGoogle Scholar
  28. Srivastava S, Conlan XA, Adholeya A, Cahill DM (2016) Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants. Plant Cell Tissue Organ Cult 126:19–32CrossRefGoogle Scholar
  29. Tepe B, Sokmen A (2007) Production and optimisation of rosmarinic acid by Satureja hortensis L. callus cultures. Nat Prod Res 21:1133–1144CrossRefGoogle Scholar
  30. Van Staden J, Harty AR (1988) Cytokinins and adventitious root formation. In: Davies TD, Haissig BE, Sankhla N (eds) Adventitious root formation in cuttings. Dioscorides Press, Portland, pp 185–201Google Scholar
  31. Yayli N, Yaşar A, Güleç C, Usta A, Kolaylı S, Coşkunçelebi K, Karaoğlu Ş (2005) Composition and antimicrobial activity of essential oils from Centaurea sessilis and Centaurea armena. Phytochemistry 66:1741–1745CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tuba Bekircan
    • 1
  • Ahmet Yaşar
    • 2
  • Sercan Yıldırım
    • 2
  • Münevver Sökmen
    • 3
    • 4
  • Atalay Sökmen
    • 3
    • 5
  1. 1.Department of Biochemistry, Faculty of ScienceAvrasya UniversityTrabzonTurkey
  2. 2.Department of Analytical Chemistry, Faculty of PharmacyKaradeniz Technical UniversityTrabzonTurkey
  3. 3.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of Bioengineering, Faculty of EngineeringFood and Agriculture UniversityKonyaTurkey
  5. 5.Department of Plant Production and Technologies, Faculty of Natural SciencesFood and Agriculture UniversityKonyaTurkey

Personalised recommendations