Optimization of the fermentation conditions for the mutant strain of β-cyclodextrin glycosyltransferase H167C to produce cyclodextrins
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The cyclodextrin glycosyltransferase (CGTase) was used to catalyze the conversion of starch into cyclodextrins (CD) in industry. Improving the activity of CGTase to produce more CD with relative low cost is intensely interesting and has drawn wide attention. Amino acid mutation of His167 into Cys significantly enhanced β-CGTase activity; however, optimization of culture conditions for β-CGTase-H167C remains unclear. To determine this, the medium and culture conditions for β-CGTase-H167C were optimized with response surface methodology. Maximum activity of β-CGTase-H167C was obtained with the medium containing 1.1% corn starch, 4.4% corn steep liquor, 1.1% peptone, 0.02% MgSO4·7H2O and 0.1% K2HPO4·3H2O that were cultured with the initial pH 8.4, incubation temperature at 37.4 °C, with 5% inoculation size and shaking speed at 202 r/min. Under the optimal conditions, the activity of β-CGTase-H167C was up to 4355 U/mL, which is 1.93-fold in comparison with the initial activity. Our results established the promising culture strategy for the production of cyclodextrins by β-CGTase-H167C.
Keywordsβ-CGTase Optimization Medium components Culture conditions Cyclodextrins
The work was supported by Natural Science Foundation of Inner Mongolia (2015BS0312). The sponsors of financial support have no involvement in the study design, data collection and analysis, writing of the report and decision to submit the article for publication.
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Conflicts of interest
The authors declare that there is no conflict of interest regarding the publication of the paper.
- Brauman A, Keleke S, Malonga M, Miambi E, Ampe F (1996) Microbiological and biochemical characterization of cassava retting, a traditional lactic Acid fermentation for foo-foo (cassava flour) production. Appl Environ Microbiol 62:2854–2858Google Scholar
- Kimura K, Ishii Y, Kataoka S, Takano T, Yamane K (1990) Expression of the beta-cyclodextrin glucanotransferase gene of an alkalophilic Bacillus sp. #1011 in Escherichia coli cells and characterization of the synthesized enzyme. Agric Biol Chem 54:641–648Google Scholar
- Rendleman JA Jr, Knutson CA Jr (1998) Conversion of cyclodextrin into high-amylose starch of low molecular mass by means of cyclodextrin glucanotransferase. Biotechnol Appl Biochem 28(Pt 3):219–228Google Scholar