An alkaline bacterial laccase for polymerization of natural precursors for hair dye synthesis
In the present study, an extracellular alkali stable laccase (Lac DS) from Bacillus subtilis DS which has pH optima at 8.5 using p-phenylenediamine (PPD) as substrate has been reported. Lac DS retained 70% activity for 4 h at pH 8.5 and 90% activity for 24 h at 55 °C. The enzyme yield was enhanced by optimization of fermentation conditions. A 746-fold increase in yield was observed under optimized conditions using 150 µM MgSO4, 1.2% yeast extract, 0.35% tryptone, and 150 µM vanillic acid. Lac DS was used to polymerize natural dye precursor catechol, pyrogallol, syringaldehyde, syringic acid, ferulic acid and gallic acid to develop a range of natural hair colors such as black, golden yellow, and reddish brown. The results indicate that alkaline Lac DS is a suitable candidate to develop a user-friendly and commercially applicable hair dyeing process in the area of cosmetic industry.
KeywordsLaccase Hair dye Synthesis Polymerization PPD Natural precursors
This work was supported by University Grant Commission (UGC)-BSR F.25-1/2013-14(BSR)/7-261/2011 (BSR) Government of India, New Delhi, India.
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Conflict of interest
The authors declare that they have no conflict of interest
Human and animal rights and informed consent
Research involving Human Participants and/or Animals/Informed consent: not applicable for the study.
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