Surface-enhanced Raman scattering (SERS) performance on salbutamol detection of colloidal multi-shaped silver nanoparticles

  • Tri Bao Ngoc Pham
  • Thi Thu Thao Bui
  • Vinh Quoc Tran
  • Vinh Quang Dang
  • Long Nguyen Hoang
  • Cong Khanh TranEmail author
Original Article


Monitoring the surface-enhanced Raman scattering (SERS) ability of multi-shaped silver nanoparticles (MAgNPs) in comparison with silver nanospheres (AgNSs), we carried out the synthesis of MAgNPs from the AgNS seeds via the etching-growth process. The MAgNPs contained diverse anisotropic shapes such as hexagonal, circular, triangular and deformed plates as well as particles in the wide range of sizes from 30 to 250 nm. The SERS spectra of salbutamol (Sal) in the presence of colloid MAgNPs, showing higher sensitivity than AgNSs with a minimum detectable concentration of Sal, was determined at 2.5 and 1.25 ng/µL with colloid MAgNPs on glass and Si/SiO2 substrates, respectively. These results pointed out the shape of silver nanoparticles (AgNPs) and their plasmonic resonance strongly affected by SERS performance. This study contributed to the development of the further facile and low-cost method for the SERS detection of Sal based on AgNPs.


Surface-enhanced Raman scattering Multi-shaped silver nanoparticles Salbutamol Colloid Anisotropic 



Surface-enhanced Raman scattering






Multi-shaped silver nanoparticles


Silver nanoparticles


Silver nanospheres


Transmission electron microscopy






Inductively coupled plasma-mass spectrometer


Methyl orange


X-ray diffraction


Bending vibration


Stretching vibration


Raman shift


The standard deviation


Enhancement factor

ζ potential

Zeta potential



The authors acknowledge Mr. Khang Duy Vu Nguyen, Institute of Applied Material Science in HCM city for his help and good advice in all measurements also useful discussions in data analyses of Raman spectra and ICP-MS results. The many thanks would be given to Prof. Van Thanh Thi Tran, the University of Science in HCM city for material support. This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant number 103.03-2018.59.

Author contributions

The authors contributed equally in the manuscripts.

Compliance with ethical standards

Conflict of interest

The authors declare no competing of interests.

Supplementary material

13204_2019_1154_MOESM1_ESM.docx (2.6 mb)
Supplementary material 1 (DOCX 2700 kb)


  1. Ali HR, Edwards HG, Kendrick J, Scowen IJ (2009) Vibrational spectroscopic study of salbutamol hemisulphate. Drug Test Anal 1(1):51–56. Google Scholar
  2. Amendola V, Bakr OM, Stellacci F (2010) A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: effect of shape, size, structure, and assembly. Plasmonics 5(1):85–97. Google Scholar
  3. Benz F, Chikkaraddy R, Salmon A, Ohadi H, de Nijs B, Mertens J, Carnegie C, Bowman RW, Baumberg JJ (2016) SERS of individual nanoparticles on a mirror: size does matter, but so does shape. J Phys Chem Lett 7(12):2264–2269. Google Scholar
  4. Caban M, Stepnowski P, Kwiatkowski M, Migowska N, Kumirska J (2011) Determination of β-blockers and β-agonists using gas chromatography and gas chromatography–mass spectrometry—a comparative study of the derivatization step. J Chromatogr A 1218(44):8110–8122. Google Scholar
  5. Cao G, Huang Z, Miu X, Sun Y, Li Y, Chen J, Lei J (2017) Chen R Silver nanoparticle-treated paper as a surface-enhanced Raman scattering (SERS) substrate for seminal plasma analysis. Optical Society of America, p W3A.12.
  6. Chen H, Wei G, Ispas A, Hickey SG, Eychmüller A (2010) Synthesis of palladium nanoparticles and their applications for surface-enhanced raman scattering and electrocatalysis. J Phys Chem C 114(50):21976–21981. Google Scholar
  7. Cheng J, Su X-O, Han C, Wang S, Wang P, Zhang S, Xie J (2018) Ultrasensitive detection of salbutamol in animal urine by immunomagnetic bead treatment coupling with surface-enhanced Raman spectroscopy. Sens Actuators B Chem 255:2329–2338. Google Scholar
  8. Cyriac J, Wleklinski M, Li G, Gao L, Cooks RG (2012) In situ Raman spectroscopy of surfaces modified by ion soft landing. Analyst 137(6):1363–1369. Google Scholar
  9. Das R, Soni RK (2017) Synthesis and surface-enhanced Raman scattering of indium nanotriangles and nanowires. RSC Adv 7(51):32255–32263. Google Scholar
  10. Davis E, Loiacono R, Summers RJ (2008) The rush to adrenaline: drugs in sport acting on the β-adrenergic system. Br J Pharmacol 154(3):584–597. Google Scholar
  11. Dong X, Ji X, Jing J, Li M, Li J, Yang W (2010) Synthesis of triangular silver nanoprisms by stepwise reduction of sodium borohydride and trisodium citrate. J Phys Chem C 114(5):2070–2074. Google Scholar
  12. Gao H, Han J, Yang S, Wang Z, Wang L, Fu Z (2014) Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol. Anal Chim Acta 839:91–96. Google Scholar
  13. Garssen GJ, Geesink GH, Hoving-Bolink AH, Verplanke JC (1995) Effects of dietary clenbuterol and salbutamol on meat quality in veal calves. Meat Sci 40(3):337–350. Google Scholar
  14. Ge J, Li Y, Wang J, Pu Y, Xue W, Liu X (2016) Green synthesis of graphene quantum dots and silver nanoparticles compounds with excellent surface enhanced Raman scattering performance. J Alloys Compd 663:166–171. Google Scholar
  15. Guo C, Shi F, Gong L, Tan H, Hu D, Zhang J (2015) Ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken by ultrahigh-performance liquid-chromatography–quadrupole-orbitrap tandem mass spectrometry. J Pharm Biomed Anal 107:526–534. Google Scholar
  16. Gwamuri J, Ragavendran V, Sadatgol M, Mayandi J, Güney D, Pearce J (2017) Ambiance-dependent agglomeration and surface-enhanced Raman spectroscopy response of self-assembled silver nanoparticles for plasmonic photovoltaic devices. J Photonics Energy. Google Scholar
  17. Haber J, Sokolov K (2017) Synthesis of stable citrate-capped silver nanoprisms. Langmuir 33(40):10525–10530. Google Scholar
  18. Hou W, Liu X, Lu Q, Liu M, Zhang Y, Yao S (2018) Etching and anti-etching strategy for sensitive colorimetric sensing of H2O2 and biothiols based on silver/carbon nanomaterial. Colloids Surf B 162:118–125. Google Scholar
  19. Izquierdo-Lorenzo I, Sanchez-Cortes S, Garcia-Ramos JV (2010) Adsorption of beta-adrenergic agonists used in sport doping on metal nanoparticles: a detection study based on surface-enhanced Raman scattering. Langmuir 26(18):14663–14670. Google Scholar
  20. Jenkins SV, Qu H, Mudalige T, Ingle TM, Wang R, Wang F, Howard PC, Chen J, Zhang Y (2015) Rapid determination of plasmonic nanoparticle agglomeration status in blood. Biomaterials 51:226–237. Google Scholar
  21. Jensen L, Aikens CM, Schatz GC (2008) Electronic structure methods for studying surface-enhanced Raman scattering. Chem Soc Rev 37(5):1061–1073. Google Scholar
  22. Jia H, Xu W, An J, Li D, Zhao B (2006) A simple method to synthesize triangular silver nanoparticles by light irradiation. Spectrochim Acta A Mol Biomol Spectrosc 64(4):956–960. Google Scholar
  23. Jiang Y, Wang J, Malfatti L, Carboni D, Senes N, Innocenzi P (2018) Highly durable graphene-mediated surface enhanced Raman scattering (G-SERS) nanocomposites for molecular detection. Appl Surf Sci 450:451–460. Google Scholar
  24. Kaur A, Gupta U (2009) A review on applications of nanoparticles for the preconcentration of environmental pollutants. J Mater Chem 19(44):8279–8289. Google Scholar
  25. Kazemzadeh Y, Shojaei S, Riazi M, Sharifi M (2019) Review on application of nanoparticles for EOR purposes: a critical review of the opportunities and challenges. Chin J Chem Eng 27(2):237–246. Google Scholar
  26. Kim MH, Kwak SK, Im SH, Lee J-B, Choi K-Y, Byun D-J (2014) Maneuvering the growth of silver nanoplates: use of halide ions to promote vertical growth. J Mater Chem C 2(30):6165–6170. Google Scholar
  27. Kwon Y-H, Ossig R, Hubenthal F, Kronfeldt H-D (2012) Influence of surface plasmon resonance wavelength on SERS activity of naturally grown silver nanoparticles ensemble. J Raman Spectrosc. Google Scholar
  28. Li H, Xia H, Wang D, Tao X (2013) Simple synthesis of monodisperse, quasi-spherical, citrate-stabilized silver nanocrystals in water. Langmuir 29(16):5074–5079. Google Scholar
  29. Li Y, Zhao X, Zhang P, Ning J, Li J, Su Z, Wei G (2015) A facile fabrication of large-scale reduced graphene oxide–silver nanoparticle hybrid film as a highly active surface-enhanced Raman scattering substrate. J Mater Chem C 3(16):4126–4133. Google Scholar
  30. Mikoliunaite L, Rodriguez RD, Sheremet E, Kolchuzhin V, Mehner J, Ramanavicius A, Zahn DRT (2015) The substrate matters in the Raman spectroscopy analysis of cells. Sci Rep 5:13150. Google Scholar
  31. Mobasser S, Firoozi A (2016) Review of nanotechnology applications in science and engineering. J Civ Eng Urban 6(4):84–93Google Scholar
  32. Muehlethaler C, Leona M, Lombardi JR (2016) Review of surface enhanced Raman scattering applications in forensic science. Anal Chem 88(1):152–169. Google Scholar
  33. Mulvihill MJ, Ling XY, Henzie J, Yang P (2010) Anisotropic etching of silver nanoparticles for plasmonic structures capable of single-particle SERS. J Am Chem Soc 132(1):268–274. Google Scholar
  34. Muniz-Miranda M, Gellini C, Giorgetti E (2011) Surface-enhanced raman scattering from copper nanoparticles obtained by laser ablation. J Phys Chem C 115(12):5021–5027. Google Scholar
  35. Ooi ME, Sayuti M, Sarhan AAD (2015) Fuzzy logic-based approach to investigate the novel uses of nano suspended lubrication in precise machining of aerospace AL tempered grade 6061. J Clean Prod 89:286–295. Google Scholar
  36. Parnklang T, Lertvachirapaiboon C, Pienpinijtham P, Wongravee K, Thammacharoen C, Ekgasit S (2013) H2O2-triggered shape transformation of silver nanospheres to nanoprisms with controllable longitudinal LSPR wavelengths. RSC Adv 3(31):12886–12894. Google Scholar
  37. Pastoriza-Santos I, Hamanaka Y, Fukuta K, Nakamura A, Liz-MarzáN LM (2003) Anisotropic silver nanoparticles: synthesis and optical properties. In: Liz-Marzán LM, Giersig M (eds) Low-dimensional systems: theory, preparation, and some applications. Springer Netherlands, Dordrecht, pp 65–75. Google Scholar
  38. Prakash Sharma V, Sharma U, Chattopadhyay M, Shukla VN (2018) Advance applications of nanomaterials: a review. Mater Today Proc 5(2, Part 1):6376–6380. Google Scholar
  39. Qian H, Anwer S, Bharath G, Iqbal S, Chen L (2018) Nanoporous Ag–Au bimetallic triangular nanoprisms synthesized by galvanic replacement for plasmonic applications. J Nanomater 2018:1–7. Google Scholar
  40. Sharma B, Frontiera RR, Henry A-I, Ringe E, Van Duyne RP (2012) SERS: materials, applications, and the future. Mater Today 15(1):16–25. Google Scholar
  41. Si MZ, Kang YP, Zhang ZG (2009) Surface-enhanced Raman scattering (SERS) spectra of methyl orange in Ag colloids prepared by electrolysis method. Appl Surf Sci 255(11):6007–6010. Google Scholar
  42. Stiles PL, Dieringer JA, Shah NC, Van Duyne RP (2008) Surface-enhanced raman spectroscopy. Annu Rev Anal Chem 1(1):601–626. Google Scholar
  43. Tanvi Mahajan A, Bedi RK, Kumar S, Saxena V, Singh A, Aswal DK (2016) Broadband enhancement in absorption cross-section of N719 dye using different anisotropic shaped single crystalline silver nanoparticles. RSC Adv 6(53):48064–48071. Google Scholar
  44. Vodnik V, Bozanic D, Bibić N, Saponjic Z, Nedeljković J (2008) Optical properties of shaped silver nanoparticles. J Nanosci Nanotechnol. Google Scholar
  45. Wan F, Shi H, Chen W, Gu Z, Du L, Wang P, Wang J, Huang Y (2017) Charge transfer effect on raman and surface enhanced Raman spectroscopy of furfural molecules. Nanomaterials. Google Scholar
  46. Wang B, Zhang Z, Chang K, Cui J, Rosenkranz A, Yu J, Lin CT, Chen G, Zang K, Luo J, Jiang N, Guo D (2018) New deformation-induced nanostructure in silicon. Nano Lett 18(7):4611–4617. Google Scholar
  47. Wei C, Li M, Zhao X (2018) Surface-enhanced Raman scattering (SERS) with silver nano substrates synthesized by microwave for rapid detection of foodborne pathogens. Front Microbiol 9:2857. Google Scholar
  48. Wu J, Yang X, Fang J (2019) Convective combined interfacial assembly of surfactantless ordered Au nanoparticles and SERS performance. J Nanoparticle Res 21(4):78. Google Scholar
  49. Wyrzykowski D, Chmurzyński L (2010) Thermodynamics of citrate complexation with Mn2+, Co2+, Ni2+ and Zn2+ ions. J Therm Anal Calorim 102(1):61–64. Google Scholar
  50. Yazdi SH, Giles KL, White IM (2013) Multiplexed detection of DNA sequences using a competitive displacement assay in a microfluidic SERRS-based device. Anal Chem 85(21):10605–10611. Google Scholar
  51. Yu Y, Wang Y, Lin K, Hu N, Zhou X, Liu S (2013) Complete Raman spectral assignment of methanol in the C-H stretching region. J Phys Chem A 117(21):4377–4384. Google Scholar
  52. Yu S, Liu Z, Zhang J, Li H, Xu N, Yuan X-x, Wu Y (2018) An azo-coupling reaction-based surface enhanced resonance Raman scattering approach for ultrasensitive detection of salbutamol. RSC Adv 8(10):5536–5541. Google Scholar
  53. Zhang Z, Huo F, Zhang X, Guo D (2012a) Fabrication and size prediction of crystalline nanoparticles of silicon induced by nanogrinding with ultrafine diamond grits. Scripta Mater 67(7–8):657–660. Google Scholar
  54. Zhang Z, Song Y, Xu C, Guo D (2012b) A novel model for undeformed nanometer chips of soft-brittle HgCdTe films induced by ultrafine diamond grits. Scripta Mater 67(2):197–200. Google Scholar
  55. Zhang Z, Wang B, Kang R, Zhang B, Guo D (2015) Changes in surface layer of silicon wafers from diamond scratching. CIRP Ann 64(1):349–352. Google Scholar
  56. Zhang Z, Wang B, Zhou P, Guo D, Kang R, Zhang B (2016a) A novel approach of chemical mechanical polishing using environment-friendly slurry for mercury cadmium telluride semiconductors. Sci Rep 6:22466. Google Scholar
  57. Zhang Z, Wang B, Zhou P, Kang R, Zhang B, Guo D (2016b) A novel approach of chemical mechanical polishing for cadmium zinc telluride wafers. Sci Rep 6:26891. Google Scholar
  58. Zhang Z, Cui J, Wang B, Wang Z, Kang R, Guo D (2017a) A novel approach of mechanical chemical grinding. J Alloys Compd 726:514–524. Google Scholar
  59. Zhang Z, Huang S, Chen L, Wang B, Wen B, Zhang B, Guo D (2017b) Ultrahigh hardness on a face-centered cubic metal. Appl Surf Sci 416:891–900. Google Scholar
  60. Zhang W, Li C, Gao K, Lu F, Liu M, Li X, Zhang L, Mao D, Gao F, Huang L, Mei T, Zhao J (2018a) Surface-enhanced Raman spectroscopy with Au-nanoparticle substrate fabricated by using femtosecond pulse. Nanotechnology 29(20):205301. Google Scholar
  61. Zhang Z, Shi Z, Du Y, Yu Z, Guo L, Guo D (2018b) A novel approach of chemical mechanical polishing for a titanium alloy using an environment-friendly slurry. Appl Surf Sci 427:409–415. Google Scholar
  62. Zhang Z, Cui J, Zhang J, Liu D, Yu Z, Guo D (2019) Environment friendly chemical mechanical polishing of copper. Appl Surf Sci 467–468:5–11. Google Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Tri Bao Ngoc Pham
    • 1
  • Thi Thu Thao Bui
    • 1
  • Vinh Quoc Tran
    • 1
  • Vinh Quang Dang
    • 1
  • Long Nguyen Hoang
    • 2
  • Cong Khanh Tran
    • 1
    Email author
  1. 1.University of ScienceHo Chi Minh CityVietnam
  2. 2.International UniversityHo Chi Minh CityVietnam

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