Mechanism of inhibition of graphene oxide/zinc oxide nanocomposite against wound infection causing pathogens

  • D. Prema
  • J. Prakash
  • S. Vignesh
  • Pandiyarasan Veluchamy
  • C. Ramachandran
  • Debashree Banita Samal
  • Deog-Hwan Oh
  • S. Sahabudeen
  • G. Devanand VenkatasubbuEmail author
Original Article


Infection and colony formation by microbes has been a negative consequence in wound care. Graphene oxide (GO) has a large surface area and it is biocompatible. Zinc oxide (ZnO) exhibits antimicrobial activity against various infectious microbes. In GO/ZnO nanocomposite, ZnO nanoparticle is homogeneously supported on the GO sheet. It controls the infection in wound area and promotes angiogenesis. The synthesized nanocomposite was characterized using XRD, FTIR, Raman Spectroscopy, UV-DRS, FESEM with EDS, TEM and ICP-OES. Antibacterial activity was studied using the well diffusion method, growth curve, death kinetics, sensitivity coefficient, biofilm inhibition and colony counting method. Reactive oxygen species assay (ROS), lactate dehydrogenase leakage assay (LDH) and DNA fragmentation studies were done to analyze the mechanism of inhibition of nanocomposite against bacteria. A hemolytic study was done to confirm the blood biocompatibility of the nanocomposites. The mechanism of bacterial inhibition is by the production of ROS, loss of membrane integrity, not by DNA fragmentation. The nanocomposites enhanced its antibacterial activity in the presence of visible light compared to dark conditions.


Graphene oxide LDH ROS DNA fragmentation Biofilm Hemolysis 



The authors D. Prema and G. Devanand Venkatasubbu would like to thank the Department of Physics and Nanotechnology, SRM Institute of Science and Technology for providing the necessary lab facilities. The authors would like to acknowledge SRM Institute of Science and Technology for providing Micro Raman facilities.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • D. Prema
    • 1
  • J. Prakash
    • 1
  • S. Vignesh
    • 2
  • Pandiyarasan Veluchamy
    • 3
  • C. Ramachandran
    • 4
  • Debashree Banita Samal
    • 5
  • Deog-Hwan Oh
    • 4
  • S. Sahabudeen
    • 2
  • G. Devanand Venkatasubbu
    • 1
    Email author
  1. 1.Department of NanotechnologySRM Institute of Science and TechnologyKattankulathurIndia
  2. 2.Department of BiotechnologySRM Institute of Science and TechnologyKattankulathurIndia
  3. 3.Department of Electronics and Communication EngineeringIndian Institute of Information Technology Design and ManufacturingKancheepuramIndia
  4. 4.Department of Food Science and Biotechnology, College of Biotechnology and BioscienceKangwon National University HyojaChuncheonSouth Korea
  5. 5.Department of MicrobiologyApollo Speciality HospitalChennaiIndia

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