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Simple fabrication of sericin/graphene nanocomposites for application in articular cartilage repair in knee joints in nursing care

  • Yanrong Yuan
  • Haizhen Liu
  • Na Zheng
  • Linggong Gao
  • Feiyan Liu
  • Guofeng Guan
  • Guilan ZhangEmail author
Original Article
  • 6 Downloads

Abstract

In the present work, sericin/reduced graphene oxide (SSG) nanocomposites are synthesized through a low cost, facile and biological approach. An anticipated amount of sericin powder was mixed to GO dispersion with sericin/GO ratios of 10:1, 50:1, and 100:1 to obtain sericin/rGO nanocomposites such as SSG10, SSG50, and SSG100, respectively. TEM images revealed the rough surface of graphene sheets with obtained proteins assembled on the surface of the GO nanosheets. Further, biological studies revealed that SSG nanocomposites significantly promoted the collagen levels and glycosaminoglycan. Cell proliferation results showed the effective growth of MSCs with SSG100 than GO, SSG10, and SSG50. Also, the confocal microscopic images exhibited the spindle-like morphology of MSCs with uniform size and increased cell density on the 5th day of cell proliferation in case of SSG100 than GO and other nanocomposites. Therefore, the present results suggest that the fabricated SSG hybrid material could act as promising candidates for future applications of repairing articular cartilage in knee joints in nursing care.

Keywords

Sericin Graphene oxide Knee joints 

Notes

References

  1. Akhavan O, Ghaderi E, Aghayee S, Fereydooni Y, Talebi A (2012) The use of a glucose-reduced graphene oxide suspension for photothermal cancer therapy. J Mater Chem 22:13773–13781CrossRefGoogle Scholar
  2. Barbero A, Grogan S, Schafer D, Heberer M, Mainil-Varlet P, Martin I (2004) Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity. Osteoarthr Cartil 12:476–484CrossRefGoogle Scholar
  3. Becerra J, Andrades JA, Guerado E, Zamora-Navas P, Lopez-Puertas JM, Reddi AH (2010) Articular cartilage: structure and regeneration. Tissue Eng Part B 16:617–627CrossRefGoogle Scholar
  4. Bhat PN, Nivedita S, Roy S (2011) Use of Bombyx mori in the synthesis of silver nanoparticle, their characterization and application. Ind J Fib Tex Res 36:168–171Google Scholar
  5. Chong Y, Ma Y, Shen H, Tu X, Zhou X, Xu J, Dai J, Fan S, Zhang Z (2014) The in vitro and in vivo toxicity of graphene quantum dots. Biomaterials 35:5041–5048CrossRefGoogle Scholar
  6. Dreyer DR, Park S, Bielawski CW, Ruoff RS (2010) The chemistry of graphene oxide. Chem Soc Rev 39:228–240CrossRefGoogle Scholar
  7. Goudarzi EHM (2014) Electro-spinning of chitosan/sericin/PVA nanoparticles incorporated in situ synthesis of nanosilver. Carbohydr Polym 113:231–239CrossRefGoogle Scholar
  8. Huey DJ, Hu JC, Athanasiou KA (2012) Unlike bone, cartilage regeneration remains elusive. Science 338:917–921CrossRefGoogle Scholar
  9. Junker JPE, Sommar P, Skog M, Johnson H, Kratz G (2010) Adipogenic, chondrogenic and osteogenic differentiation of clonally derived human dermal fibroblasts. Cells Tissues Organs 191:105–118CrossRefGoogle Scholar
  10. Khire TS, Kundu J, Kundu SC (2010) The fractal self-assembly of silk protein sericin. Soft Matter 6:2066–2071CrossRefGoogle Scholar
  11. Lee WC, Lim CHYX, Shi H, Tang LAL, Wang Y, Lim CT, Loh KP (2011) Origin of enhanced stem cell growth and differentiation on graphene and graphene oxide. ACS Nano 5:7334–7341CrossRefGoogle Scholar
  12. Lubar DR, White PH, Callahan LF, Chang RW, Helmick CGCH, Lappin DR, Melnick A, Moskowitz RW, Odom E, Sacks J, Toal SB, Waterman MB (2010) A National Public Health Agenda for Osteoarthritis 2010. Semin Arthritis Rheum 39:323–326CrossRefGoogle Scholar
  13. Nayak TR, Andersen H, Makam VS, Khaw C, Bae S, Xu X, Ee PLR, Ahn JH, Hong BH, Pastorin G, Ozyilmaz B (2011) Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 5:4670–4678CrossRefGoogle Scholar
  14. Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A (2010) Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 31:5536–5544CrossRefGoogle Scholar
  15. Pham TA, Kumar NA, Jeong YT (2010) Covalent functionalization of graphene oxide with polyglycerol and their use as templates for anchoring magnetic nanoparticles. Synth Met 16:2028–2036CrossRefGoogle Scholar
  16. Pham TA, Choi BC, Lim KT, Jeong YT (2011) A simple approach for immobilization of gold nanoparticles on graphene oxide sheets by covalent bonding Appl. Surf Sci 25:3350–3357CrossRefGoogle Scholar
  17. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147CrossRefGoogle Scholar
  18. Pornanong A, Sorada K, Phaibul P, Teerapol S (2009) Effectiveness of inflammatory cytokines induced by sericin compared to sericin in combination with silver sulfadiazine cream on wound healing. Wounds 21:198–206Google Scholar
  19. Ren PG, Yan DX, Ji X, Chen T, Li ZM (2011) Temperature dependence of graphene oxide reduced by hydrazine hydrate. Nanotechnology 22:55705–55712CrossRefGoogle Scholar
  20. Shah RN, Shah NA, Del Rosario Lim MM, Hsieh C, Nuber G, Stupp SI (2010) Supramolecular design of self-assembling nanofibers for cartilage regeneration. Proc Natl Acad Sci 107:3293–3298CrossRefGoogle Scholar
  21. Sireesh Babu M, Badal Kumar M, Shivendu R, Nandita D (2015) Diastase assisted green synthesis of size controllable gold nanoparticles. RSC Adv 5:26727–26733CrossRefGoogle Scholar
  22. Solchaga LA, Penick KJ, Welter JF (2011) Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks. Methods Mol Biol 698:253–278CrossRefGoogle Scholar
  23. Storm MM, Overgaard M, Younesi R, Reeler NEA, Vosch T, Nielson UG, Edstrom K, Norby P (2015) Reduced graphene oxide for Li–air batteries: the effect of oxidation time and reduction conditions for graphene oxide. Carbon 85:233–244CrossRefGoogle Scholar
  24. Toh WS, Lee EH, Guo XM, Chan JKY, Yeow CH, Choo AB, Cal T (2010) Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells. Biomaterials 31:6968–6980CrossRefGoogle Scholar
  25. Von Der Mark K, Gauss V, Von Der Mark H, Muller P (1977) Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 267:531–532CrossRefGoogle Scholar
  26. Wang Y, Blasioli DJ, Kim HJ, Kim HS, Kaplan DL (2006) Cartilage tissue engineering with silk scaffolds and human articular chondrocytes. Biomaterials 27:4434–4442CrossRefGoogle Scholar
  27. Yelin E, Weinstein S, King T (2016) The burden of musculoskeletal diseases in the United States. Semin Arthritis Rheum 46:259CrossRefGoogle Scholar
  28. Yoon HH, Bhang SH, Kim T, Yu T, Hyeon T, Kim BS (2014) Dual roles of graphene oxide in chondrogenic differentiation of adult stem cells: cell-adhesion substrate and growth factor-delivery carrier. Adv Funct Mater 24:6455–6464CrossRefGoogle Scholar
  29. Yue K, Trujillo-de Santiago G, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271CrossRefGoogle Scholar
  30. Zhang L, Hu J, Athanasiou KA (2009) The role of tissue engineering in articular cartilage repair and regeneration. Crit Rev Bioeng 37:1–57Google Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Yanrong Yuan
    • 1
  • Haizhen Liu
    • 2
  • Na Zheng
    • 3
  • Linggong Gao
    • 4
  • Feiyan Liu
    • 1
  • Guofeng Guan
    • 1
  • Guilan Zhang
    • 4
    • 5
    Email author
  1. 1.Department of Foot and Ankle SurgeryBinzhou Medical University HospitalBinzhouChina
  2. 2.Nursing DepartmentShi Kou Central HospitalDongyingChina
  3. 3.The Third Department of PsychiatryBinzhou People’s HospitalBinzhouChina
  4. 4.Department of Reproductive MedicineBinzhou Medical University HospitalBinzhouChina
  5. 5.Department of Hand (Micro) SurgeryBinzhou Medical University HospitalBinzhouChina

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