Applied Nanoscience

, Volume 8, Issue 7, pp 1809–1821 | Cite as

Bacillus lipopeptides: powerful capping and dispersing agents of silver nanoparticles

  • Vivek Rangarajan
  • Gunaseelan Dhanarajan
  • Pinaki Dey
  • Dipankar Chattopadhya
  • Ramkrishna SenEmail author
Original Article


This study demonstrates the use of Bacillus lipopeptides as both capping and stabilizing agents in the single-step synthesis of silver nanoparticles (Ag-NPs). With the aim to achieve a stable Ag-NP suspension, two methods of synthesis procedures, methods A and B, were tested and compared. In method A, the excess reactant sodium borohydride (NaBH4) was added to the limiting reactant silver nitrate (AgNO3), while in method B the limiting reactant was added to the excess reactant. It was found that, in both methods, the lipopeptide concentration significantly influenced the morphology and size of Ag-NPs. In case of method A, at low lipopeptide concentrations, Ag-NPs of uniform size distribution were synthesized, whereas at high concentration, incipient small nano-spheres of Ag-NPs were found concentrated at the vesicular surfaces during initial stage of reaction. However, at later stages, the smaller spherical particles radically changed into varied shapes and sizes and eventually detached from the vesicles completely. Ag-NPs of method B showed narrow size distribution. Stability studies revealed that Ag-NP suspension remained stable up to the sodium chloride, NaCl, concentration of 25 g/L. Steric and depletion types of stabilization were found to be the predominant mechanisms that imparted stability to the Ag-NPs. The particles were found to be stable up to test period of 6 months. Antimicrobial studies revealed that the lipopeptide-conjugated Ag-NPs with low lipopeptide were sensitive against all the tested organisms.


Lipopeptide Silver nanoparticles Capping agents Nanoparticle stability Antimicrobial activity 


Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

Supplementary material

13204_2018_852_MOESM1_ESM.docx (906 kb)
Supplementary material 1 (DOCX 905 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Vivek Rangarajan
    • 1
    • 2
  • Gunaseelan Dhanarajan
    • 1
  • Pinaki Dey
    • 3
  • Dipankar Chattopadhya
    • 4
  • Ramkrishna Sen
    • 1
    Email author
  1. 1.Department of BiotechnologyIndian Institute of Technology KharagpurKharagpurIndia
  2. 2.Department of Chemical EngineeringBITS PilaniZuarinagarIndia
  3. 3.Department of Biosciences and TechnologyKarunya UniversityCoimbatoreIndia
  4. 4.Department of Polymer Science and TechnologyUniversity of CalcuttaCalcuttaIndia

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