Prolonged and continuous antibacterial and anti-biofilm activities of thin films embedded with gentamicin-loaded mesoporous silica nanoparticles
- 11 Downloads
The application of mesoporous silica nanoparticles (MSNs) in drug delivery systems has become highly attractive since the early 2000s. In this study, thin-film coatings embedded with gentamicin-loaded mesoporous silica nanoparticles (MSN-G) were prepared to provide antibacterial and anti-biofilm activity over a prolonged period of time. The prolonged and continuous activity of MSN-G films against Staphylococcus aureus throughout the release period was studied via two methods, namely, (1) disc diffusion of released gentamicin and (2) by shifting the MSN-G thin film to a new agar plate at certain time intervals. The expansion of the inhibition zone from 4.6 ± 0.5 to 9.7 ± 0.5 mm as caused by the released fraction of gentamicin from the first week to the eighth week indicated the controlled and slow release behaviour of loaded antibiotic and prolonged antibacterial efficacy of these films. In addition, the appearance of an inhibition zone after each shifting of the film to a new agar plate was persistent up to 103 days which confirmed that thin films successively prevented bacterial growth over a long period of time. In addition, the anti-biofilm activity of MSN-G films was evaluated by imaging bacterial cells attachment via confocal laser scanning microscopy and scanning electron microscopy. Remarkably, the anti-biofilm performance remained active for more than 2 months. To the best of our knowledge, such a slow and controlled release of antibiotic from nanoparticle embedded thin films with uninterrupted, continuous, and prolonged antibacterial effect for more than 2 months has not been reported yet.
KeywordsThin-film drug delivery systems Mesoporous silica nanoparticles Gentamicin S. aureus Antibacterial activity
T. Tamanna acknowledges Australian Government Research Training Program Scholarship for supporting this work. C. B. Landersdorfer is an Australian National Health and Medical Research Council (NHMRC) Career Development Fellow (APP1062509).
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Adeshina G, Onujagbe O, Onaolapo J (2010) Comparative antibacterial studies on the root, stem bark and leaf extracts of Parkia clappertoniana. Internet J Altern Med 2:1–12Google Scholar
- Adeshina G, Kunle O, Onaolapo J, Ehinmidu J, Odama L (2012) Phytochemical and antibacterial studies of the hexane extract of Alchornea cordifolia leaf. In: Rao V (ed) Phytochemicals as nutraceuticals-global approaches to their role in nutrition and health. InTech, Rijeka, pp 89–96Google Scholar
- Massa MA, Covarrubias C, Bittner M, Fuentevilla IA, Capetillo P, Von Marttens A, Carvajal JC (2014) Synthesis of new antibacterial composite coating for titanium based on highly ordered nanoporous silica and silver nanoparticles. Mater Sci Eng C 45:146–153. https://doi.org/10.1016/j.msec.2014.08.057 CrossRefGoogle Scholar
- Sombié BC, Yameogo BGJ, Semdé R, Henschel V, Amighi K, Goole J (2014) Stability study in accelerated conditions of gentamicin–glyceryl monooleate–water based gel used in the treatment of chronic osteomyelitis. Am J Adv Drug Deliv 2:203–212Google Scholar
- Zimmerli W, Sendi P (2011) Pathogenesis of implant-associated infection: the role of the host, vol 33. Springer, BerlinGoogle Scholar