Biosynthesis of silver nanoparticles using bark extracts of Butea monosperma (Lam.) Taub. and study of their antimicrobial activity

Original Article
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Abstract

Biosynthesis of silver nanoparticles was achieved using bark extract of Butea monosperma (Lam.) Taub., a native plant of Indian subcontinent and southeast Asia. The plant parts are familiar for ailment of different diseases. The bioactive compounds present in bark of the plant were extracted with Soxhlet extractor. Silver nitrate (AgNO3) was used as a raw material for preparation of silver nanoparticles (AgNPs). The ratio of bark extract and silver nitrate solution for synthesis of AgNPs was standardized as 3:5. The change in colour of the solution from pale yellow to deep brown can be correlated to reduction reaction catalyzed by plant bioactive compounds. The formation of AgNPs was confirmed by UV–Vis spectrophotometer. The surface plasmon resonance (SPR) maxima, λmax, were recorded at 452 nm. SPR indicates the nature and type of particles present in the solution. The suitable concentration of AgNO3 was found to be 10 mM to carry out reduction reaction with the bark extract. Alkaline environment (pH 9) suitably promotes the reaction. FTIR graph of synthesized AgNPs shows the shifting peak of 3265.0 wavelength/cm and 1635.40 wavelength/cm indicates that AgNPs were coated with plant biomolecules, which is attributed to the stabilization of AgNPs. XRD and SEM photograph of the AgNPs showed that they were spherical in shape and capped with bioactive compounds. Thus, the synthesized AgNPs are more stable, less toxic and homogenous in shape. The average diameter of the nanoparticles was 81 nm. The synthesized AgNPs had efficacy against a Gram-negative bacteria (Escherichia coli), a Gram-positive bacteria (Staphylococcus aureus), and a mold (Aspergillus niger). The maximum conversion was 66%. From the present investigation, it can be concluded that the bioactive compounds present in the bark of Butea have the capacity to reduce silver ion into silver nanoparticles in aqueous condition and the synthesized AgNPs are stabilized and loss toxic. Moreover, they also possess antimicrobial properties against human pathogens.

Keywords

Green synthesis Silver nanoparticles Bark extract Butea monosperma Antimicrobial properties 

Notes

Acknowledgements

The authors are highly grateful to management of GIET, Gunupur for providing laboratory facilities. The help rendered by the Director, CIPET, Bhubaneswar, during the analysis of silver nanoparticles is highly acknowledged.

References

  1. Akira I, Masashige S, Hiroyuki H, Takeshi K (2005) Medical application of functionalized magnetic nanoparticles. Biosci Bioeng 100(1):1–11CrossRefGoogle Scholar
  2. Banerjee P, Satpathy M, Mukhopadhayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess 1:1–10CrossRefGoogle Scholar
  3. Bhainsa KC, D’Souza SF (2006) Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigates. Coll Surf B Biointerfaces 47:160–164CrossRefGoogle Scholar
  4. Bhargava SK (1986) Estrogenic and postcoital anticonceptive activity in rats of butin isolated from Butea monosperma seed. J Ethnopharm 18(1):95–101CrossRefGoogle Scholar
  5. Bodroth RP, Das M (2012) Phytochemical Screening and antimicrobial activity of ethanol and chloroform extract of Ziziphus nummularia Wt. & Arm. African. J Biotech. 11(21):4929–4933Google Scholar
  6. Chaudhuri SK, Malodia L (2017) Biosynthesis of zinc oxide nanoparticles using leaf extract of Calotropis gigantea: characterization and its evaluation on tree seedling growth in nursery stage. Appl Nanosci 7:501–512CrossRefGoogle Scholar
  7. Daniele V, Claudia B, Xingcai Z, Viviana V, Andrea T, Vito L, Ross R, Yuri ML, Stefano L, Michele M (2012) Lapatinib/Paclitaxel polyelectrolyte nanocapsules for overcoming multidrug resistance in ovarian cancer. Nanomed Nanotechnol Bio Med 8(6):891–899CrossRefGoogle Scholar
  8. Fayaz M, Tiwary CS, Kalaichelvan PT, Venkatesan R (2010) Blue orange light emission from biogenic synthesized silver nanoparticles using Trichoderma viride. Coll Surf B Biointerfaces 75(1):175–178CrossRefGoogle Scholar
  9. Jain P, Pradeep T (2005) Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter. J Biotechnol Bioeng 90:59–63CrossRefGoogle Scholar
  10. Kasture VS, Chopde CT, Deshmukh VK (2000) Anticonvulsive activity of Albizia lebbeck, Hibiscus rosasinensis and Butea monosperma in experimental animals. J Ethnopharmaco 71(1–2):65–75CrossRefGoogle Scholar
  11. Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K (2011) Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 79:594–598CrossRefGoogle Scholar
  12. Maria BS, Devadiga A, Kodialbail VS, Saidutta MB (2015) Synthesis of silver nanoparticles using medicinal Zizyphus xylopyrus bark extract. Appl Nanosci 5:755–762CrossRefGoogle Scholar
  13. Mishra M, Shukla YN, Kumar S (2000) Euphane triterpenoid and lipid constituents from Butea monosperma. Phytochem 54(8):835–838CrossRefGoogle Scholar
  14. Patil RS, Kokate MR, Kolekar SS (2012) Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 91:234–238CrossRefGoogle Scholar
  15. Pattekari P, Zheng Z, Zhang X, Levchenko T, Torchilin V, Lvov Y (2011) Top-down and bottom-up approaches in production of aqueous nanocolloids of low solubility drug paclitaxel. Phys Chem Chem Phys 13:9014–9019CrossRefGoogle Scholar
  16. Philip D (2011) Mangifera Indica leaf-assisted biosynthesis of well-dispersed silver nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc 8:327–333CrossRefGoogle Scholar
  17. Philip D, Aswathy Unni C, Aromal S, Vidhu VK (2011) Murraya koenigii leaf-assisted rapid green synthesis of silver and gold nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc 78:899–904CrossRefGoogle Scholar
  18. Sahar MO (2014) Antifungal activity of silver and copper nano particles on two plant pathogens, Alternaria alternate and Botrytis cinerea. Res J Microbio 9:34–42CrossRefGoogle Scholar
  19. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Coll Surf B Biointerfaces 73:332–338CrossRefGoogle Scholar
  20. Sehrawat A, Kumar V (2012) Butein imparts free radical scavenging, anti-oxidative and pro apoptotic properties in the flower extracts of Butea monosperma. Biocell 36(2):63–71Google Scholar
  21. Shankar SS, Ahmed A, Sastry M (2003) Geranium leaf assisted biosynthesis of silver nanoparticles. Biotech Prog 19:1627–1631CrossRefGoogle Scholar
  22. Sharma D, Das M (2010) Efficacy of antimicrobial metabolites of Pseudomonas fluorescens PFG against phytopathogenic fungi. Proc National Acad Sci India. 80:68–71Google Scholar
  23. Sindhura KS, Prasad TN, Selvam P, Hussain OM (2014) Synthesis, characterization and evaluation of effect of phytogenic zinc nanoparticles on soil exoenzymes. Appl Nanosci 4:819–827CrossRefGoogle Scholar
  24. Sumitra M, Manikandan P, Suguna L (2005) Efficacy of Butea monosperma on dermal wound healing in rats. Int J Biochem Cell Biol 37(3):566–573CrossRefGoogle Scholar
  25. Tarafdar JC, Xiang Y, Wang WN, Dong Q, Biswas P (2012) Standardization of size, shape and concentration of nanoparticle for plant application. Appl Biol Res 14:138–144Google Scholar
  26. Viviana V, Flavia S, Claudia B, Rosaria R, Daniele V, Michele M, Francesca B, Gianluigi G, Xingcai Z, Yuri ML, Stefano L (2011) Drug-loaded polyelectrolyte microcapsules for sustained targeting of cancer cells. Adv Drug Deliv Rev 63(9):847–864CrossRefGoogle Scholar
  27. Weicong M, Yuan Z, Chuanfa L, Jinlun W, Kunyi L, Xingcai Z, Ruliang L, Ruowen F, Dingcai W (2017) Functional nanonetwork-structured polymers with inbuilt poly(acrylic acid) linings for enhanced adsorption. Polym Chem 8:4771–4775CrossRefGoogle Scholar
  28. Xia L, Lenaghan SC, Zhang M, Zhang Z, Li Q (2010) Naturally occurring nanoparticles from English Ivy: an alternative to metal based nanoparticles for UV protection. J Nanobiotech 8:12CrossRefGoogle Scholar
  29. Yadava RN, Reddy KI (1998) A new bio-active flavonol glycoside from the stems of Butea superba Roxb. J Asian Nat Prod Res. 1(2):139–145CrossRefGoogle Scholar
  30. Yang D, Zhike H, Shuyi W, Kairong X, Xingcai Z, Bingna Z, Reddeppa N, Ruowen F, Dingcai W (2018) Preparation of versatile yolk-shell nanoparticles with a precious metal yolk and a microporous polymer shell for high-performance catalysts and antibacterial agents. Polymer 137:195–200CrossRefGoogle Scholar
  31. Yoon WJ, Jung KY, Liu J, Duraisamy T, Revur R, Teixeira FL, Berger PR (2010) Plasmon-enhanced optical absorption and photocurrent in organic bulk hetero junction photovoltaic devices using self-assembled layer of silver nanoparticles. Sol Energy Matter Sol Cells 94:128–132CrossRefGoogle Scholar
  32. Yuanyuan Y, Peng J, Xingcai Z, Nagaiya R, Hao Y, Chenghuan Y, Huazhong Y, Yongquan X, Junfeng Y, Kai W, Ming W, Qizhen D (2017) New Epigallocatechin Gallate (EGCG) nanocomplexes co-assembled with 3-Mercapto-1-Hexanol and β-lactoglobulin for improvement of antitumor activity. J Biomed Nanotechnol 13(7):805–814CrossRefGoogle Scholar
  33. Yuri ML, Pravin P, Xingcai Z, Vladimir T (2011) Converting poorly soluble materials into stable aqueous nanocolloids. Langmuir 27(3):1212–1217CrossRefGoogle Scholar
  34. Zhong XW, Xian HY, Feng L, Fen YK, Wei XL, Wei W (2018) Multiplexed ratiometric photoluminescent detection of pyrophosphate using anisotropic boron-doped nitrogen-rich carbon rugby ball-like nanodots. J Mater Chem B 5 (in press)Google Scholar

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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyGandhi Institute of Engineering and TechnologyGunupurIndia

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