Soil phosphorus availability is a driver of the responses of maize (Zea mays) to elevated CO2 concentration and arbuscular mycorrhizal colonisation
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In the context of a changing climate, there has been an increased research focus on predicting the response of plants to elevated atmospheric CO2 concentrations. Simultaneously, the availability of phosphate fertiliser is becoming increasingly limited. Although the effects of climate change on arbuscular mycorrhizal fungi (AMF) have been given some attention, it has not been possible to generalise effects of AMF confidently across plant functional groups such as C3 and C4 plants. We grew the C4 cereal Zea mays cv. B73 with or without inoculation with the AMF Funneliformis mosseae, at three different soil P concentrations ranging from 0 to 90 mg P kg−1 soil, and at ambient (400 ppm: aCO2) or elevated (900 ppm: eCO2) CO2 concentrations. Plant biomass, shoot P nutrition, and colonisation by AMF were analysed. With limiting soil P, plant growth responded positively to colonisation by AMF, but not eCO2. At the medium soil P treatment, shoot P concentration but not growth was increased by AMF colonisation, while growth was instead increased by eCO2. The positive growth response to eCO2 persisted at the highest soil P treatment, where there were no effects of AMF colonisation on plant growth or P nutrition. The effects of AMF colonisation and CO2 on maize growth and P nutrition in a future climate scenario will likely be highly dependent on the availability of soil P. Furthermore, differential effects on plants of contrasting functional groups (eg., C3 vs C4) should be considered when investigating the role of AMF under eCO2 conditions.
KeywordsArbuscular mycorrhizal symbiosis C4 photosynthesis Elevated atmospheric CO2 Plant phosphorus uptake Soil phosphorus Zea mays
The authors wish to thank Dr. Olena Kravchuk for statistical consultation, Mette Flodgaard for excellent technical assistance, and two reviewers whose comments greatly improved the manuscript. Funding for this work was provided by The Danish Council for Independent Research — Technology and Production Sciences, grant 0602-01412B. SJWW also wishes to acknowledge support from the University of Adelaide Ramsay Fellowship.
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