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Symbiosis

, Volume 76, Issue 1, pp 41–49 | Cite as

The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model

  • Mariana L. Puente
  • José L. Gualpa
  • Gastón A. Lopez
  • Romina M. Molina
  • Susana M. Carletti
  • Fabricio D. Cassán
Article

Abstract

Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC ) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment.

Keywords

Azospirillum Indole-3-acetic acid Soybean Foliar inoculation Bradyrhizobium 

Notes

Acknowledgements

We thank Universidad Nacional de Río Cuarto, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Fondo Nacional de Ciencia y Tecnología (FONCyT) and Instituto Nacional de Tecnología Agropecuaria (INTA, Argentina). Fabricio Cassán is a Researcher of CONICET at the Universidad Nacional de Río Cuarto. Gaston Lopez is a postdoctoral researcher; José Gualpa and Romina Molina are PhD students at the Universidad Nacional de Río Cuarto and granted by CONICET.

Compliance with ethical standards

Conflicts of interest

The authors report no conflicts of interest.

Supplementary material

13199_2017_536_MOESM1_ESM.docx (15 kb)
ESM 1 Table S1 Primers used in this study. (DOCX 14 kb)
13199_2017_536_MOESM2_ESM.docx (757 kb)
ESM 2 Fig. S1: Scanning electron microscopy (SEM) of soybean leaf showing A. brasilense Az39 colonizing the surface of leaves 48 h after inoculation. The white arrows indicate the presence of bacteria on the plant tissue. A and B represent different fractions of the leaf. Colonization ability was confirmed for both A. brasilense Az39 wt and the IAA–deficient mutant ipdC (not shown). (DOCX 756 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Mariana L. Puente
    • 1
  • José L. Gualpa
    • 2
  • Gastón A. Lopez
    • 2
  • Romina M. Molina
    • 2
  • Susana M. Carletti
    • 3
  • Fabricio D. Cassán
    • 2
  1. 1.Laboratorio de Bacterias Promotoras del Crecimiento Vegetal, Instituto de Microbiología y Zoología Agrícola IMYZAINTA CastelarBuenos AiresArgentina
  2. 2.Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo, Departamento de Ciencias Naturales, FCEFQyNUniversidad Nacional de Río CuartoCórdobaArgentina
  3. 3.Departamento de Ciencias BásicasUniversidad Nacional de LujánBuenos AiresArgentina

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