, Volume 76, Issue 1, pp 1–12 | Cite as

Ectomycorrhizas of two species of Tuber (clade Puberulum) in the Mexican subtropical cloud forest

  • Antero Ramos
  • Edith Garay-Serrano
  • Enrique César
  • Leticia Montoya
  • Víctor M. BandalaEmail author


Tuber species are hypogeous mycorrhizal symbionts distributed almost worldwide and associated with several tree families. In this study, we recorded the occurrence of two Tuber species (clade Puberulum) in the Acatlán Volcano (Veracruz, Eastern Mexico). They were detected by the means of the internal transcribed spacer (ITS) of nuclear ribosomal DNA sequences obtained from ectomycorrhizal samples collected at the site. The Tuber ectomycorrhizas were related symbiotically with Alnus acuminata and Fagus grandifolia var. mexicana. This is the first report of Tuber associated with these trees. The anatomical and morphological characterization of the ectomycorrhizae are presented along with illustrations.


Ascomycota Mycorrhizal interactions Hypogeous fungi Cryptic species Endangered tree species 



We appreciate the assistance in the field and in the laboratory by Biols. D. Ramos and J.C. Corona (both at Instituto de Ecología, A.C.). We recognize the support given by CONACYT (CB 169172) to study the EcM fungi associated with Fagus grandifolia var. mexicana in the montane cloud forest of Central Veracruz and by the postdoctoral grant to A. Ramos. We acknowledge the support given by CONACYT (225382) to the Laboratorio de Presecuenciación, Red Biodiversidad y Sistemática, INECOL. M.Sc. Bertha Pérez at this lab aid in some molecular procedures. We are thankful to the anonymous reviewers and the editor, Professor David Richardson, for their helpful suggestions on the manuscript.

Compliance with ethical standards


All the experiments undertaken in this study comply with the current laws of the country where they were performed.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13199_2017_535_MOESM1_ESM.xlsx (37 kb)
ESM 1 (XLSX 36 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Antero Ramos
    • 1
  • Edith Garay-Serrano
    • 1
  • Enrique César
    • 1
  • Leticia Montoya
    • 1
  • Víctor M. Bandala
    • 1
    Email author
  1. 1.Red Biodiversidad y SistemáticaInstituto de EcologíaVeracruzMexico

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