Jamun fruit (Syzgium cumini) skin extract based indicator for monitoring chicken patties quality during storage

  • S. TalukderEmail author
  • S. K. Mendiratta
  • R. R. Kumar
  • R. K. Agrawal
  • A. Soni
  • A. Luke
  • S. Chand
Original Article


Natural plant pigment, anthocyanins have the capability to change its color with the change of its structure influenced by changing pH. This feature of anthocyanin has been harnessed to design a meat products quality indicator. In the present experiment anthocyanin rich Jamun fruit (Syzgium cumini) skin extract was used to develop quality indicator by immobilizing on filter paper strips with the purpose of application in chicken patties packets stored at refrigeration temperature (4 ± 1 °C). The indicator changed its color from violet to yellow due to changed pH in it when it was attached inside packet of chicken patties during storage, due to reaction with volatile basic compounds generated from meat. During storage for 21 days, various changes in quality attributes of chicken patties viz., pH, Total volatile basic nitrogen (TVBN), ammonia level, color value, sensory attributes and microbial evaluation were estimated. The pH decreased (P < 0.5) from 6.22 to 6.04. TVBN and ammonia and level increased significantly (P < 0.5) throughout storage. Redness, yellowness, hue and chroma value gradually changed during storage. Sensory scores also decreased significantly (P < 0.5). Microbial count also increased (P < 0.5) during this time. The experiment showed that, during storage, the color changing pattern of quality indicator was well correlated with the changes in quality attributes of chicken meat patties. Therefore, it is expected that the developed quality indicator can provide a convenient, non destructive, visual mean to monitor the meat products quality during refrigerated storage.


Jamun fruit skin extracts Anthocyanin Quality indicator Chicken patties quality 



This study was funded by Ministry of Food Processing Industries (SERB/MOFPI/0019/2014).

Compliance with ethical standards

Conflict of interest

Author Dr. Suman Talukder declares that he has no conflict of interest. Co-author Dr. S. K. Mendiratta declares that he has no conflict of interest. Co-author Dr. R. R. Kumar declares that he has no conflict of interest. Co-author Dr. R. K. Agrawal declares that he has no conflict of interest. Co-author Dr. A. Soni declares that he has no conflict of interest. Co-author Ashish Luke declares that he has no conflict of interest. Co-author Dr. Sagar Chand declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.


  1. Ali EH (2005) Effects of some amino acids on ammonia secretion and extracellular protease activity by three oomycetes in synthetic medium with or without glucose. Mycobiology 33(1):23–29CrossRefGoogle Scholar
  2. APHA (2001) Compendium of methods for microbiological examination of food, 4th edn. American Public Health Association, WashingtonGoogle Scholar
  3. Baldevbhai PJ, Anand RS (2012) Color Image segmentation for medical images using L*a*b* Color space. IOSR J Electro Commun Eng 1(2):24–45CrossRefGoogle Scholar
  4. Berry BW, Stiffler DM (1981) Effect of electric stimulator boiling temperature formulation and rate of freezing on sensory cooking chemical and physical properties of ground beef patties. J Food Sci 46:1103–1106CrossRefGoogle Scholar
  5. Bhat ZF, Pathak V, Fayaz H (2013) Effect of refrigerated storage on the quality characteristics of microwave cooked chicken Seekh Kababs extended with different non-meat proteins. J Food Sci Technol 50(5):926–933CrossRefGoogle Scholar
  6. Biswas O, Talwar NA, Bhattacharya D, Das SK, Chaudhary S (2017) Effect of refrigeration on quality and stability of fresh fish and poultry muscles. Indian J Anim Health 56(1):65–76Google Scholar
  7. Bruckner S, Albrecht A, Petersen B, Kreyenschmidt J (2012) characterization and comparison of spoilage processes in fresh pork and poultry. J Food Quality 35:372–382CrossRefGoogle Scholar
  8. Byun JS, Min JS, Kim IS, Kim JW, Chung MS, Lee M (2003) Comparison of indicators of microbial quality of meat during aerobic cold storage. J Food Protect 66:1733–1737CrossRefGoogle Scholar
  9. Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42CrossRefGoogle Scholar
  10. Fernandez-lopez J, Zhi N, Aleson-carbonell L, Perez-alvarez JA, Kuri V (2005) Antioxidant and antibacterial activities of natural extracts: application in beef meatballs. Meat Sci 69:371–380CrossRefGoogle Scholar
  11. Froehlich DA, Gullet EA, Usborne WR (1983) Effect of nitrite and salt on the color, flavor and overall acceptability of ham. J Food Sci 48(1):152–154CrossRefGoogle Scholar
  12. Ghollasi-Mood F, Mohsenzadeh M, Reza Housaindokht M, Varidi M (2016) Microbial and chemical spoilage of chicken meat during storage at isothermal and fluctuation temperature under aerobic conditions. Iran J Vet Sci Technol 8(1):38–46Google Scholar
  13. Golasz LB, Da Silva L, Da Silva SB (2013) Film with anthocyanins as an indicator of chilled pork deterioration. Cienc Tecnol Aliment Campinas 33(1):155–162CrossRefGoogle Scholar
  14. Goulas AE, Chouliara I, Nessi E, Kontominas MG, Savvaidis IN (2005) Microbiological, biochemical and sensory assessment of mussels (Mytilus galloprovincialis) stored under modified atmosphere packaging. J Appl Microbiol 98:752–760CrossRefGoogle Scholar
  15. He J, Giusti MM (2010) Anthocyanins: natural colorants with health-promoting properties. Annu Rev Food Sci Technol 1:163–187CrossRefGoogle Scholar
  16. Jay JM (1996) Modern food microbiology, 4th edn. CBS Publishers and Distributors, New DelhiCrossRefGoogle Scholar
  17. Karamucki T, Jakubowska M, Rybarczyk A, Gardzielewska J (2013) The influence of myoglobin on the colour of minced pork loin. Meat Sci 94:234–238CrossRefGoogle Scholar
  18. Keeton JT (1983) Effect of fat and NaCl/phosphate levels on the chemical and sensory properties of pork patties. J Food Sci 48(3):871–881, 885Google Scholar
  19. Khare AK, Biswas AK, Balasubramanium S, Chatli MK, Sahoo J (2015) Optimization of meat level and processing conditions for development of chicken meat noodles using response surface methodology. J Food Sci Technol 52(6):3719–3729Google Scholar
  20. Kuswandi B, Damayanti F, Jayus AA, Heng LY (2015) Simple and low-cost on-package sticker sensor based on litmus paper for real-time monitoring of beef freshness. J Math Fund Sci 47(3):236–251CrossRefGoogle Scholar
  21. Little AC (1975) Off on a tangent. J Food Sci 40:410–412CrossRefGoogle Scholar
  22. Metivier RP, Francis FJ, Clydesdale FM (1980) Solvent extraction of anthocyanin from wild pomace. J Food Sci 45:1099–1100CrossRefGoogle Scholar
  23. Moldovanu C, Laslo C (2010) Physicochemical and microbiological research on characteristics of meat products during storage inthe membrane depending on the quality of rawmaterials. ABAH Bioflu 2(2):65–70Google Scholar
  24. Nwosu FO, Adekola FA, Ihedioha KC (2004) simple titrimetric colour indicators from some natural flower petals. Centrepoint 12(1):74–89Google Scholar
  25. Nychas GJE, Skandamis PN, Tassou CC, Koutsoumanis KP (2008) Meat spoilage during distribution. Meat Sci 78:77–89CrossRefGoogle Scholar
  26. Pathade KS, Patil SB, Konda-war MS, Naik-wade NS, Magdum CS (2009) Morus alba fruit-herbal alternative to synthetic acid base indicators. Int J Chem Tech Res 1(3):549–551Google Scholar
  27. Patsias A, Badeka AV, Savvaidis IN, Kontominas MG (2008) Combined effect of freeze chilling and MAP on quality parameters of raw chicken fillets. Food Microbiol 25:575–581CrossRefGoogle Scholar
  28. Pearson D (1968) Methods related to protein break down. J Sci Food Agric 19(3):366–369CrossRefGoogle Scholar
  29. Rosen H (1957) A modified ninhydrin colorimetric analysis for amino acids. Archiv Biochem Biophys 67(1):10–15CrossRefGoogle Scholar
  30. Russell SM, Fletcher DL, Cox NA (2005) Spoilage bacteria of fresh broiler chicken carcasses. Poult Sci 74(12):2041–2047CrossRefGoogle Scholar
  31. Sastry VRB, Kamra DN, Pathak NN (1999) Estimation of ammonia nitrogen. Laboratory Manual of Animal Nutrition, Indian Veterinary Research Institute, IzatnagarGoogle Scholar
  32. Shelef LA, Jay JM (1970) Use of titrimetric method to assess the bacterial spoilage of fresh beef. Appl Microbiol 19:902–905Google Scholar
  33. Shukla V, Kandeepan G, Vishnuraj MR (2015) Evaluation of shelf life of buffalo meat in aerobic cold storage using physicochemical parameters. Buff Bull 34(4):453–457Google Scholar
  34. Singh BP, Shukla V, Lalawmpuii H, Kumar S (2018) Indicator sensors for monitoring meat quality: a review. J Pharmacogn Phytochem 7(4):809–812Google Scholar
  35. Soni A, Mendiratta SK, Talukder S, Kumar RR, Chand S, Jaiswal RK (2018) Development of colorimetric on-package indicator for monitoring of chicken meat freshness during refrigerated storage (4 ± 1°C). J Anim Res 8(5):847–853CrossRefGoogle Scholar
  36. Strange ED, Benedict RC, Smith JL, Swift CE (1977) Evaluation of rapid test for monitoring alteration in meat quality during storage. J Food Protect 40:843–847CrossRefGoogle Scholar
  37. Talukder S, Mendiratta SK (2017) Exploring purple leaf sand cherry (Prunus cistena) extracts based indicator to monitor meat quality during storage at 10 ± 1° C. Int J Livest Res 8(7):214–220Google Scholar
  38. Talukder S, Mendiratta SK, Kumar RR, Agarwal RK, Soni A, Chand S, Singh TP, Sharma H (2017) Development of plant extracts based indicator for monitoring quality of fresh chicken meat during storage at room temperature (25 ± 1° C). J Anim Res 7(4):751–755CrossRefGoogle Scholar
  39. Trout ES, Hunt MC, Jhnson DE, Claus JR, Kastner CL, Kropt DH (1992) Characteristics of low fat ground beef containing texture modifying ingredients. J Food Sci 57:19–24CrossRefGoogle Scholar
  40. Walkowiak-Tomczak D, Czapski J, Mlynarczyk K (2016) Assessment of colour changes during storage of elderberry juice concentrate solutions using the optimization method. Acta Sci Pol Technol Aliment 15(3):299–309CrossRefGoogle Scholar
  41. Washko ME, Rice EW (1961) Determination of glucose by an improved enzymatic procedure. Clin Chem 7:542–545Google Scholar
  42. Yusoff A, Kumara NTRN, Lim A, Ekanayake P, Tennakoon KU (2014) Impacts of temperature on the stability of tropical plant pigments as sensitizers for dye sensitized solar cells. J Biophys 1:1–8CrossRefGoogle Scholar
  43. Zulfajri M, Muttakin M (2018) Activity analysis of anthocyanin from Syzygium cumini (L.) skeels as a natural indicator in acid-base titration. Rasayan J Chem 11(1):135–141Google Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Division of Livestock Products TechnologyICAR-Indian Veterinary Research InstituteIzatnagar, BareillyIndia

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