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Identification and quantitation of genetically modified (GM) ingredients in maize, rice, soybean and wheat-containing retail foods and feeds in Turkey

Abstract

The cultivation area and diversity of genetically modified (GM) crop varieties worldwide is increasing rapidly. Taking Turkey as an example of a country with tight restrictions on the import and use of GM crops but limited resources for product monitoring, we developed a cost-effective 3-tier screening protocol, and tested 110 retail food products and 13 animal feeds available in 2016–2017 for GM ingredients. No evidence was found for the presence of GM wheat or rice in the foodstuffs tested; however, 6 feeds and 3 food products containing soybean and/or maize were positive for one or more GM elements. GM events present in positive samples were identified by event-specific PCR and quantified by real-time PCR. We also compared the results with previous surveys in Turkey. Overall, we observed consistent use of GM animal feeds; however, these were not labelled as GM at the point of sale. Occasional food products also tested positive for GM ingredients, usually at low concentrations that could be attributed to accidental contamination.

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Abbreviations

CRM:

Certified reference material

CTAB:

Cetyltrimethylammonium bromide

FAM:

6-Carboxyfluorescein

GMO:

Genetically modified organism

qPCR:

Quantitative polymerase chain reaction

RRS:

Roundup ready soybean

SSII:

Starch synthase II

SPS:

Sucrose phosphate synthase

TAMRA:

Carboxytetramethylrhodamine

References

  1. Akpinar BA, Avsar B, Lucas SJ, Budak H (2012) Plant abiotic stress signaling. Plant Signal Behav 7:1450–1455. https://doi.org/10.4161/psb.21894

  2. Al-Hmoud N, Al-Husseini N, Ibrahim-Alobaide MA et al (2014) Unconventional P-35S sequence identified in genetically modified maize. GM Crops Food 5:58–64. https://doi.org/10.4161/gmcr.27542

  3. Arvas Y, Kaya M (2018) Detection of genetically modified organisms in rice products by DNA-based techniques in Turkish Market. In: 1. International Agricultural Science Congress. Van Yuzuncu Yıl University, Van

  4. Elsanhoty RM, Al-Turki AI, Ramadan MF (2013) Prevalence of genetically modified rice, maize, and soy in Saudi food products. Appl Biochem Biotechnol 171:883–899. https://doi.org/10.1007/s12010-013-0405-x

  5. Erkan I, Dastan K (2017) Real-time PCR detection of genetically modified organisms in several food products and their environmental effects in Turkey. Fresenius Environ Bull 26:2589–2595

  6. European Commission (2003a) Regulation (EC) No 1829/2003 of the European Parliament. Off J Eur Union L268:1–23

  7. European Commission (2003b) Regulation (EC) No 1830/2003 of the European Parliament. Off J Eur Union L268:24–28

  8. Fraiture M-A, Herman P, Taverniers I et al (2015) Current and new approaches in GMO detection: challenges and solutions. Biomed Res Int 2015:1–22. https://doi.org/10.1155/2015/392872

  9. Gürakan GC, Aydin G, Yilmaz R (2011) Qualitative detection of GM maize (Bt11) in food and feed sold commercially in Turkey by PCR based methods. Indian J Biotechnol 10:143–146

  10. Lamichhane SA (2014) Genetically modified foods - Solution for food security. IJGEB 5:43–48

  11. Leitch IJ, Johnston E, Pellicer J et al. (2019) Angiosperm DNA C-values database (release 9.0, Apr 2019). https://cvalues.science.kew.org/. Accessed 13 Sept 2019

  12. Lucas SJ (2018) The application of nucleic acid microarrays for the detection of genetically modified organisms. In: Mahgoub SEO, Nollet L (eds) Testing and analysis of GMO-containing foods and feed. CRC Press, London, pp 205–222

  13. Mandaci M, Çakir Ö, Turgut-Kara N et al (2014) Detection of genetically modified organisms in soy products sold in Turkish market. Food Sci Technol. https://doi.org/10.1590/1678-457X.6441

  14. Özgen Arun Ö, Yilmaz F, Muratoĝlu K (2013) PCR detection of genetically modified maize and soy in mildly and highly processed foods. Food Control 32:525–531. https://doi.org/10.1016/j.foodcont.2013.01.023

  15. Tengel C, Schüssler P, Setzke E et al (2001) PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs. Biotechniques 31:426–429

  16. Turkec A, Lucas SJ, Karlik E (2016a) Monitoring the prevalence of genetically modified (GM) soybean in Turkish food and feed products. Food Control 59:766–772. https://doi.org/10.1016/j.foodcont.2015.06.052

  17. Turkec A, Lucas SJ, Karlik E (2016b) Monitoring the prevalence of genetically modified maize in commercial animal feeds and food products in Turkey. J Sci Food Agric 96:3173–3179. https://doi.org/10.1002/jsfa.7496

  18. Wunderlich S, Gatto KA (2015) Consumer perception of genetically modified organisms and sources of information. Adv Nutr 6:842–851. https://doi.org/10.3945/an.115.008870

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Acknowledgements

This research was financially supported by an internal grant from Uludağ University Scientific Research Projects Unit (Grant Number 2014/1) and by Nilay Agricultural Products Ltd.

Author information

BA conducted experiments, analyzed data and drafted the manuscript. SS collected samples and conducted experiments. AT conceived the study, collected samples and assisted with data analysis. SJL planned the experimental work, analyzed data and revised the manuscript. All authors approved the final manuscript.

Correspondence to Stuart J. Lucas.

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Avsar, B., Sadeghi, S., Turkec, A. et al. Identification and quantitation of genetically modified (GM) ingredients in maize, rice, soybean and wheat-containing retail foods and feeds in Turkey. J Food Sci Technol 57, 787–793 (2020). https://doi.org/10.1007/s13197-019-04080-2

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Keywords

  • Genetically modified organisms
  • Food
  • Feed
  • Food safety
  • Real-time PCR
  • GMO screening