Journal of Food Science and Technology

, Volume 56, Issue 12, pp 5194–5203 | Cite as

Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic

  • Prince ChawlaEmail author
  • Naveen Kumar
  • Ravinder Kaushik
  • Sanju B. Dhull
Original Article


To assess the cellular mineral uptake and oxidative stability of flower extract, a nanoscale gum arabic stabilized Rhododendron arboreum flower extract emulsion was formulated. Four different concentrations of flower extract (1–5%) were used for the optimization of the nanoemulsion. A significant (P < 0.05) difference was observed in average droplet size (43.51–55.87 nm) of the nanoemulsion. FTIR spectrum confirmed mainly C=C, aliphatic C–H, aliphatic and aromatic galacto-proteins, and polymeric-OH groups present in nanoemulsion. Smooth type of nanoemulsion was confirmed by inverted light microscopy. Ionic strength was evaluated and significant (P < 0.05) increase in particles size was attributed, whereas significant (P < 0.05) decrease in zeta potential was observed by increased NaCl concentration. Iron and calcium showed a non-significant difference in terms of mineral bioavailability. Calcium revealed significantly higher cellular uptake (52.11%) in comparison with iron (50.25%) and zinc (45.32%) during transwell assay. Higher cellular iron uptake unveiled a satisfactory amount of ferritin content


Gum arabic Nanoemulsion Rhododendron arboreum TBA Minerals FTIR 



Flower extract




Dulbecco’s modified eagles medium


Phosphate buffer saline


Atomic absorbance spectrophotometer


Dynamic light scattering


Fourier transform infrared spectroscopy


Thiobarbituric acid



The support by School of Bioengineering and Food Technology, Shoolini University, Solan, Himachal Pradesh, India is gratefully acknowledged.


  1. Ammar I, Ennouri M, Attia H (2015) Phenolic content and antioxidant activity of cactus (Opuntia ficus-indica L.) flowers are modified according to the extraction method. Ind Crop Product 64:97–104CrossRefGoogle Scholar
  2. Bhattacharyya D (2011) Rhododendron species and their uses with special reference to Himalayas—a review. Assam Uni J of Sci Tech 7(1):161–167Google Scholar
  3. Campelo PH, Junqueira LA, Resende JVD, Zacarias RD, Fernandes RVDB, Botrel DA, Borges SV (2017) Stability of lime essential oil emulsion prepared using biopolymers and ultrasound treatment. Int J Food Prop 20(1):S564–S579CrossRefGoogle Scholar
  4. Carpenter J, Saharan VK (2017) Ultrasonic assisted formation and stability of mustard oil in water nanoemulsion: effect of process parameters and their optimization. Ultrason Sonochem 35:422–430CrossRefGoogle Scholar
  5. Chawla P, Bhandari L, Sadh PK, Kaushik R (2017) Impact of solid-state fermentation (Aspergillus oryzae) on functional properties and mineral bioavailability of black-eyed pea (Vigna unguiculata) seed flour. Cereal Chem 94(3):437–442CrossRefGoogle Scholar
  6. Fernandes L, Saraiva JA, Pereira JA, Casal S, Ramalhosa E (2018) Post-harvest technologies applied to edible flowers: a review: edible flowers preservation. Food Rev Int 32:1–23Google Scholar
  7. García-Lafuente A, Guillamón E, Villares A, Rostagno MA, Martínez JA (2009) Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm Res 58(9):537–552CrossRefGoogle Scholar
  8. Gescher K, Kühn J, Hafezi W, Louis A, Derksen A, Deters A, Hensel A (2011) Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1. Fitoterapia 82(3):408–413CrossRefGoogle Scholar
  9. Guan Y, Wu J, Zhong Q (2016) Eugenol improves physical and chemical stabilities of nanoemulsions loaded with β-carotene. Food Chem 194:787–796CrossRefGoogle Scholar
  10. Gupta C, Chawla P, Arora S, Tomar SK, Singh AK (2015) Iron microencapsulation with blend of gum arabic, maltodextrin and modified starch using modified solvent evaporation method–milk fortification. Food Hydrocoll 43:622–628CrossRefGoogle Scholar
  11. Ha TVA, Kim S, Choi Y, Kwak HS, Lee SJ, Wen J, Ko S (2015) Antioxidant activity and bioaccessibility of size-different nanoemulsions for lycopene-enriched tomato extract. Food Chem 178:115–121CrossRefGoogle Scholar
  12. Jayme ML, Dunstan DE, Gee ML (1999) Zeta potentials of gum arabic stabilised oil in water emulsions. Food Hydrocoll 13(6):459–465CrossRefGoogle Scholar
  13. Kaushik R, Sachdeva B, Arora S (2015) Heat stability and thermal properties of calcium fortified milk. Cyta J Food 13(2):305–311CrossRefGoogle Scholar
  14. Kim SJ, Um JY, Hong SH, Lee JY (2011) Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-κB activation in mouse peritoneal macrophages. Am J Chin Med 39(01):171–181CrossRefGoogle Scholar
  15. Kiruba S, Mahesh M, Nisha SR, Paul ZM, Jeeva S (2011) Phytochemical analysis of the flower extracts of RA Sm. ssp. nilagiricum (Zenker) Tagg. Asian Pac J Trop Biomed 1(2):284–286CrossRefGoogle Scholar
  16. Kumar V, Suri S, Prasad R, Gat Y, Sangma C, Jakhu H, Sharma M (2019) Bioactive compounds, health benefits and utilization of Rhododendron: a comprehensive review. Agri Food Security 8(1):6CrossRefGoogle Scholar
  17. Li J, Hwang IC, Chen X, Park HJ (2016) Effects of chitosan coating on curcumin loaded nano-emulsion: study on stability and in vitro digestibility. Food Hydrocoll 60:138–147CrossRefGoogle Scholar
  18. Louis A, Petereit F, Lechtenberg M, Deters A, Hensel A (2010) Introduction. Planta Med 76:1550–1557CrossRefGoogle Scholar
  19. Pal S, Bhattacharjee P (2018) Spray dried powder of lutein-rich supercritical carbon dioxide extract of gamma-irradiated marigold flowers: process optimization, characterization and food application. Powder Tech 327:512–523CrossRefGoogle Scholar
  20. Popescu R, Kopp B (2017) The genus Rhododendron: an ethnopharmacological and toxicological review. J Ethnopharma 147(1):42–62CrossRefGoogle Scholar
  21. Qiang Y, Zhou B, Gao K (2011) Chemical constituents of plants from the genus Rhododendron. Chem Biodiv 8(5):792–815CrossRefGoogle Scholar
  22. Rachmawati H, Novel MA, Nisa RM, Berlian G, Tandrasasmita OM, Rahma A, Tjandrawinata RR (2017) Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis. J Drug Delivery Sci Tech 39:123–130CrossRefGoogle Scholar
  23. Ranjitkar S, Kindt R, Sujakhu NM, Hart R, Guo W, Yang X, Luedeling E (2014) Separation of the bioclimatic spaces of Himalayan tree rhododendron species predicted by ensemble suitability models. Glob Eco Conserv 1:2–12CrossRefGoogle Scholar
  24. Sadh PK, Chawla P, Bhandari L, Kaushik R, Duhan JS (2017) In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. J food Sci Tech 54(11):3640–3649CrossRefGoogle Scholar
  25. Sari TP, Mann B, Kumar R, Singh RRB, Sharma R, Bhardwaj M, Athira S (2015) Preparation and characterization of nanoemulsion encapsulating curcumin. Food Hydrocoll 43:540–546CrossRefGoogle Scholar
  26. Sharma A, Shree BS, Arora S, Kapila S (2017) Preparation of lactose-iron complex and its cyto-toxicity, in vitro digestion and bioaccessibility in Caco-2 cell model system. Food Bioscience 20:125–130CrossRefGoogle Scholar
  27. Taarji N, da Silva CAR, Khalid N, Gadhi C, Hafidi A, Kobayashi I, Nakajima M (2018) Formulation and stabilization of oil-in-water nanoemulsions using a saponins-rich extract from argan oil press-cake. Food Chem 246:457–463CrossRefGoogle Scholar
  28. Verma N, Singh AP, Amresh G, Sahu PK, Rao CV (2010) Anti-inflammatory and anti-nociceptive activity of RA. J Pharm Res 3:1376–1380Google Scholar
  29. Zhang XN, Li JM, Yang Q, Feng B, Liu SB, Xu ZH, Guo YY, Zhao MG (2010) Anti-apoptotic effects of hyperoside via inhibition of NR2B-containing NMDA receptors. Pharmacological reports 62(5):949–955CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Shoolini UniversitySolanIndia
  2. 2.Amity UniversityJaipurIndia
  3. 3.Chaudhary Devi Lal UniversitySirsaIndia
  4. 4.Lovely Professional UniversityPhagwaraIndia
  5. 5.Amity UniversityNoidaIndia

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