Journal of Food Science and Technology

, Volume 56, Issue 11, pp 5056–5062 | Cite as

Effects of the extraction method and chromatographic separation solvent in the antioxidant activity of different species of “espinheira-santa”

  • Tatiane Priscila ChiapettiEmail author
  • Ubirajara Contro Malavasi
  • Gilberto Costa Braga
  • Marlene Matos Malavasi
Original Article


Bioactivity of “espinheira-santa” leaves can be evaluated through solvent purification processes. Species of the same genus may contain different compounds, which may be separated by conventional chromatography using different solvents. The extraction process also influences the contents of these compounds. In order to optimize the above process research is required on variables such as solvents, temperature and extraction time. The objective of this work was to evaluate the antioxidant activity (ABTS, DPPH and FRAP) of extracts and purified fractions from leaves of Maytenus ilicifolia and M. aquafolium. Percolation at 28 °C for 7 days, and ultrasound at 50, 60 and 70 °C for 15 min were the extraction methods tested. Open column chromatography (silica gel) was used in the fractionation of extracts, and hexane, chloroform and methanol were the elution solvents used. The evaluation methods of the antioxidant activity were DPPH, FRAP and ABTS. Plant species showed no significant differences between methods for DPPH, but the fractions obtained with chromatography had significant differences, the crude ethanolic extract (CEE) and the methanolic been in general superior. “espinheira-santa” is a plant with antioxidant potential, but the extraction methods are not optimal. Still, it can be said that the separation by chromatography provided a better visualization of the nature of the components of that plant that best stood out in relation to the antioxidant activity.


Extraction methods Chromatography Antioxidant activity Bioactivity 



  1. Atl M (2016) Effect of different extraction techniques on the yield, antioxidant activity, total dosages, and profile by hplc-dad of Dicksonia sellowiana (Presl.). Hook, dicksoniaceae. Rev Bras Plantas Med 18(1983–084x):230–239. CrossRefGoogle Scholar
  2. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 239:70–76. CrossRefPubMedPubMedCentralGoogle Scholar
  3. Brand-Willian W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28(1):25–30. CrossRefGoogle Scholar
  4. Bressani R (1993) Grain quality of common beans. Food Rev Int 9(2):237–297. CrossRefGoogle Scholar
  5. Cunico MM, Cirio GM, Miguel OG, Miguel MD, Montrucchio DP, Auer CG, Grigoletti Júnior A (2002) Study of antifungal activity of Maytenus ilicifolia Mart. ex Reiss., Celastraceae. Rev Bras Farmacogn 12(2):69–73. CrossRefGoogle Scholar
  6. de Souza LM, Cipriani TR, Serrato RV, da Costa DE, Lacomini M, Gorin PAJ, Sassaki GL (2008) Analysis of flavonol glycoside isomers from leaves of Maytenus ilicifolia by offline and online high performance liquid chromatography-electrospray mass spectrometry. J Chromatogr 1207(1–2):101–109. CrossRefGoogle Scholar
  7. Ferreira DF (2000) Análise estatística por meio do SISVAR (Sistema para Análise de Variância) para Windows versão 4.0. In: REUNIÃO ANUAL DA REGIÃO BRASILEIRA DA SOCIEDADE INTERNACIONAL DE BIOMETRIA, vol 45. UFSCar, Anais, São Carlos, pp 255–258Google Scholar
  8. Gomes MF, Silva VTB, Laverde A Jr, Takemura OS (2008) Avaliação da atividade antioxidante de extratos das folhas de Bixa orellana (Bixaceae) e Maytenus ilicifolia (Celastraceae). Arq Ciênc Saúde Unipar 12(3):169–173Google Scholar
  9. Haida KS, Haas J, de Lima DS, Haida KY, da Silva FJ, Limana S, Rodrigues RT (2012) Anti-oxidant activity and phenolic compounds of Maytenus ilicifolia and Maytenus aquifolium. Rev Saúde Pesqui 5(2):360–368Google Scholar
  10. Mariot MP, Barbieri RL (2007) O Conhecimento Popular Associado ao Uso da Espinheira-santa (Maytenus ilicifolia e M aquifolium). Revis Bras Biociênc 5(1):666–668Google Scholar
  11. Mokrani A, Madani K (2016) Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Sep Purif Technol 162(January):68–76. CrossRefGoogle Scholar
  12. Negri MLS, Possamai JC, Nakashima T (2009) Antioxidant activity of “espinheira-santa” - Maytenus ilicifolia Mart. ex Reiss., leaves dried in different temperatures. Braz J Pharmacogn 19(2B):553–556. CrossRefGoogle Scholar
  13. Perez-Jiménez J, Saura-Calixto F (2006) Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res Int 39:791–800. CrossRefGoogle Scholar
  14. Radomski MI, Scheffer MC (2004) Características Fenotípicas de 44 Progênies de Maytenus ilicifolia Mart. Cultivadas no Município de Ponta Grossa, PR. Colombo: Embrapa Florestas, 2004. 6p (Embrapa Florestas. Circular técnica, 86)Google Scholar
  15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Riceevans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9–10):1231–1237. CrossRefPubMedPubMedCentralGoogle Scholar
  16. Silva MR, Da Silva MAAP (1999) Aspectos nutricionais de fitatos e taninos. Rev Nutr 12(1):21–32. CrossRefGoogle Scholar
  17. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical screening and extraction - a review. Int Pharm Sci 1(1):98–106Google Scholar
  18. Vellosa JCR, Barbosa VF, Khalil NM, Santos VAFFM, Furlan M, Lourenço I, Oliveira OMMF (2007) Profile of Maytenus aquifolium action over free radicals ande reactive oxygen species. Braz J Pharm Sci São Paulo 43(3)Google Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Tatiane Priscila Chiapetti
    • 1
    Email author
  • Ubirajara Contro Malavasi
    • 2
  • Gilberto Costa Braga
    • 2
  • Marlene Matos Malavasi
    • 2
  1. 1.Marechal Cândido Rondon - Center of Agrarian SciencesWest State University of ParanáBarracãoBrazil
  2. 2.Marechal Cândido Rondon - Center of Agrarian SciencesWest State University of ParanáMarechal Cândido RondonBrazil

Personalised recommendations