Journal of Food Science and Technology

, Volume 56, Issue 11, pp 4946–4955 | Cite as

Effects on quality properties of cooked pork sausages with Caesalpinia sappan L. extract during cold storage

  • Jin-Kyu Seo
  • Rashida Parvin
  • Dong-Gyun Yim
  • Md Ashrafuzzaman Zahid
  • Han-Sul YangEmail author
Original Article


This study was evaluated the possibility of replacing some chemical additives in meat products by the Caesalpinia sappan L. (CS) extract. Four different types of cooked pork sausages were prepared by mixing nitrite with CS extracts at different concentration like control (without nitrite and extract), T1 (0.007% nitrite), T2 (0.004% nitrite + 0.05% extract) and T3 (0.1% extract). Physicochemical properties, sensory attributes, and antioxidant activity of the control and treatments were investigated. Ash contents and DPPH radical scavenging activity were significantly (P < 0.05) higher when pork sausages were processed with the addition of CS extracts. The rate of lipid oxidation significantly (P < 0.05) decreased in the case of CS extracts addition. By comparing with the control, all nitrite or CS extract resulted in significant (P < 0.05) lower L* values; however, 0.007% nitrite and 0.004% nitrite plus 0.05% CS extract pointed significantly (P < 0.05) higher a* values in all storage times. As well as, arrangement of CS extracts (0.05%) and nitrite (0.004%) in pork sausages displayed high gumminess and cohesiveness values (P < 0.05), stable springiness and chewiness, and significantly (P < 0.05) enhance overall acceptability scores as sensory attributes compared to the control sausage after 30 days of storage. Therefore, the CS extract (0.05%), which can act as a natural antioxidant, exert a positive effect with nitrite (0.004%) on the sensory acceptability and help to preserve the desired color of cooked pork sausages by reducing oxidation rate during cold storage.


Cooked pork sausage Caesalpinia sappan L. extract Nitrite replacement Lipid oxidation Antioxidant activity Physicochemical quality 



This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food Agriculture, Forestry and Fisheries, Ministry of Agriculture, Food and Rural Affairs (Project No. 316064-02-2-HD030).


  1. A.O.A.C 17th edn (2000) Official methods of analysis. Association of Official Analytical Chemists, Gaithersburg, MDGoogle Scholar
  2. Badami S, Moorkoth S, Rai SR, Kannan E, Bhojrai S (2003) Antioxidant activity of Caesalpinia sappan heartwood. Biol Pharm Bull 26:1534–1537CrossRefGoogle Scholar
  3. Biswas AK, Keshri RC, Bisht GS (2004) Effect of enrobing and antioxidants on quality characteristics of precooked pork patties under chilled and frozen storage conditions. Meat Sci 66(3):733–741CrossRefGoogle Scholar
  4. Bourne MC (1978) Texture profile analysis. Food Technol 32:62–66Google Scholar
  5. Brannan R (2009) Effect of grape seed extract on descriptive sensory analysis of ground chicken during refrigerated storage. Meat Sci 81:589–595CrossRefGoogle Scholar
  6. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310CrossRefGoogle Scholar
  7. CIE (Commission Internationale de l’Eclairage) (1978) Recommendations on uniform color spaces–color difference equations, psychometric color terms. Supplement No. 2 to CIE Publication No. 15 (E-1.3.1) 1971/(TC-1–3). ParisGoogle Scholar
  8. Estévez M (2011) Review protein carbonyls in meat systems: a review. Meat Sci 89:259–279CrossRefGoogle Scholar
  9. Estévez M, Ventanas S, Cava R (2005) Protein oxidation in frankfurters with increasing levels of added rosemary essential oil: effect on color and texture deterioration. J Food Sci 70(7):427–432CrossRefGoogle Scholar
  10. Estévez M, Ventanas S, Cava R (2006) Effect of natural and synthetic antioxidants on protein oxidation and color and texture changes in refrigerated stored porcine liver pâté. Meat Sci 74:396–403CrossRefGoogle Scholar
  11. Gallego MG, Gordon MH, Segovia FJ, Almajano MP (2015) Caesalpinia decapetala extracts as inhibitors of lipid oxidation in beef patties. Molecules 20(8):13913–13926CrossRefGoogle Scholar
  12. Gu EJ, Kim DW, Jang GJ, Song SH, Lee JI, Lee SB, Kim BM, Cho Y, Lee HJ, Kim HJ (2017) Mass-based metabolomic analysis of soybean sprouts during germination. Food Chem 217:311–319CrossRefGoogle Scholar
  13. Ham YK, Hwang KE, Song DH, Kim YJ, Shin DJ, Kim K, Lee HJ, Kim NR, Kim CJ (2017) Lotus (Nelumbo nucifera) rhizome as an antioxidant dietary fiber in cooked sausage: effects on physicochemical and sensory characteristics. Korean J Food Sci Anim Resour 37(2):219–227CrossRefGoogle Scholar
  14. Honikel KO (2008) The use and control of nitrate and nitrite for the processing of meat products. Meat Sci 78:68–76CrossRefGoogle Scholar
  15. Janero DR (1990) Malonaldehyde and thiobarbituric acid-reactivity as diagnostics indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 9:515–540CrossRefGoogle Scholar
  16. Jiménez-Colmenero F, Ayo MJ, Carballo J (2005) Physicochemical properties of low sodium frankfurter with added walnut: effect of transglutaminase combined with caseinate, KCl and dietary fiber as salt replacers. Meat Sci 69:781–788CrossRefGoogle Scholar
  17. Latou E, Mexis SF, Badeka AV, Kontakos S, Kontominas MG (2014) Combined effect of chitosan and modified atmosphere packaging for shelf life extension of chicken breast fillets. Lebensm Wiss Technol 55:263–268CrossRefGoogle Scholar
  18. Lim MY, Jeon JH, Jeong EY, Lee CH, Lee HS (2007) Antimicrobial activity of 5-hydroxy-1,4-naphthoquinone isolated from Caesalpinia sappan toward intestinal bacteria. Food Chem 100:1254–1258CrossRefGoogle Scholar
  19. Lin Y, Huang M, Zhou G, Zou Y, Xu X (2011) Prooxidant effects of the combination of green tea extract and sodium nitrite for accelerating lipolysis and lipid oxidation in pepperoni during storage. J Food Sci 76:C694–C700CrossRefGoogle Scholar
  20. Lund MN, Heinonen M, Baron CP, Estévez M (2011) Protein oxidation in muscle foods: a review. Mol Nutr Food Res 55:83–95CrossRefGoogle Scholar
  21. Ma GX, Zhu YD, Sun ZH, Yuan JQ, XieY Zhang XP, Tian Y, Yang JS, Wu HF, Xu XD (2014) Three new cassane diterpenes from the seeds of Caesalpinia sappan. Phytochem Lett 8:141–144CrossRefGoogle Scholar
  22. Meilgaard M, Civille GV, Carr BT (1999) Sensory evaluation techniques, 3rd edn. CRC Press, Boca Raton, FL, p 354CrossRefGoogle Scholar
  23. Petron MJ, Raes K, Claeys E, Louren M, Fremaut D, De Smet S (2007) Effect of grazing pastures of different botanical composition on antioxidant enzyme activities and oxidative stability of lamb meat. Meat Sci 75:737–745CrossRefGoogle Scholar
  24. Ponnampalam EN, Plozza T, Kerr MG, Linden N, Mitchell M, Bekhit AA, Hopkins DL (2017) Interaction of diet and long ageing period on lipid oxidation and color stability of lamb meat. Meat Sci 129:43–49CrossRefGoogle Scholar
  25. Rial G, Boulaaba A, Popp J, Klein G (2017) Effects of parsley extract powder as an alternative for the direct addition of sodium nitrite in the production of mortadella-type sausages—impact on microbiological, physicochemical and sensory aspects. Meat Sci 131:166–175CrossRefGoogle Scholar
  26. Riazi F, Zeynail F, Hoseini E, Behmadi H, Savadkoohi S (2016) Oxidation phenomena and color properties of grape pomace on nitrite-reduced meat emulsion systems. Meat Sci 121:350–358CrossRefGoogle Scholar
  27. Safitri R, Tarigan P, Freisleben HJ, Rumampuk RJ, Murakami A (2003) Antioxidant activity in vitro of two aromatic compounds from Caesalpinia sappan L. BioDactors 19(1–2):71–77CrossRefGoogle Scholar
  28. Sebranek JG, Bacus JN (2007) Cured meat products without direct addition of nitrate or nitrite: what are the issues? Meat Sci 77:136–147CrossRefGoogle Scholar
  29. Sebranek JG, Fox JB Jr (1985) A review of nitrite and chloride chemistry: interactions and implications for cured meats. J Sci Food Agric 36:1169–1182CrossRefGoogle Scholar
  30. Sebranek JG, Jackson-Davis AL, Myers KL, Lavieri NA (2012) Beyond celery and starter culture: advances in natural/organic curing processes in the United States. Meat Sci 92:267–273CrossRefGoogle Scholar
  31. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 19:669–675CrossRefGoogle Scholar
  32. Thomas VH, Julie VB, Lynn V, Els V, John VC, Stefaan DS (2014) Nitrite curing of chicken, pork, and beef inhibits oxidation but does not affect N-nitroso compound (NOC)-specific DNA adduct formation during in vitro digestion. J Agric Food Chem 62:1980–1988CrossRefGoogle Scholar
  33. Vossen E, Utrera M, De Smet S, Morcuende D, Estévez M (2012) Dog rose (Rosa canina L.) as a functional ingredient in porcine frankfurters without added sodium ascorbate and sodium nitrite. Meat Sci 92:451–457CrossRefGoogle Scholar
  34. Youssef MK, Barbut S (2009) Effects of protein level and fat/oil on emulsion stability, texture, microstructure, and color of meat batters. Meat Sci 82:228–233CrossRefGoogle Scholar
  35. Zhang L, Lin YH, Leng XJ, Huang M, Zhou GH (2013) Effect of sage (Salvia officinalis) on the oxidative stability of Chinese-style sausage during refrigerated storage. Meat Sci 95:145–150CrossRefGoogle Scholar
  36. Zhang H, Peng X, Li X, Wu J, Guo X (2017) The application of clove extract protects Chinese-style sausages against oxidation and quality deterioration. Korean J Food Sci Anim Resour 37(1):114–122CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Division of Applied Science (BK21 Plus)Gyeongsang National UniversityJinjuKorea
  2. 2.Department of Animal ScienceSangji UniversityWonjuKorea
  3. 3.Institute of Agriculture and Life ScienceGyeongsang National UniversityJinjuKorea

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