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Journal of Food Science and Technology

, Volume 56, Issue 11, pp 5146–5151 | Cite as

Antibiotic residues and mycotoxins in raw milk in Punjab (India): a rising concern for food safety

  • Pallavi MoudgilEmail author
  • Jasbir Singh Bedi
  • Rabinder Singh Aulakh
  • Jatinder Paul Singh Gill
Short Communication

Abstract

The present study was envisaged with an aim to determine the occurrence of antibiotic residues; enrofloxacin, oxytetracycline, penicillin G, sulphamethoxazole and chloramphenicol as well as mycotoxins; aflatoxin M1 and ochratoxin A in raw milk samples collected from individual animals from dairy farms located in 9 districts of Punjab, India. A total of 168 raw milk samples were collected and analysed using commercially available competitive Enzyme linked immunosorbent assay kits. Out of these, 19 (11.3%) and 9 (5.4%) samples were found positive for antibiotic residues and mycotoxins, respectively. The milk samples were positive for enrofloxacin (4.8%), oxytetracycline (3.0%), penicillin G (3.0%) and sulphamethoxazole (0.6%) residues. The percentage of samples found above maximum residue limit established by Europen Union (EU)/Codex Alimentarius Commission (CAC) were 1.7%, 1.2% and 0.6% for enrofloxacin, oxytetracycline and penicillin G residues, respectively. Aflatoxin M1 was detected in 5.4% of monitored milk samples with 1.2% samples exceeded the established maximum levels of EU but were below the maximum levels established by CAC. The occurrence of antibiotic residues and mycotoxins in raw milk samples above maximum limits is a rising concern for food safety due to possibility of health risks to the consumers.

Keywords

Antibiotic residues Health risks Milk Mycotoxins 

Notes

Acknowledgements

Authors are thankful to “Rashtriya Krishi Vikas Yojana” (RKVY), Government of India, for providing funds for undertaking the study through project entitled “Studies on current scenario of antibiotic residues in food of animal origin in the Punjab and prevention of antibiotic residue risks”.

References

  1. Abebew D, Belihu K, Zewde G (2014) Detection and determination of oxytetracycline and penicillin G antibiotic residue levels in bovine bulk milk from Nazareth dairy farms. Ethiop Vet J 18:1–15Google Scholar
  2. Ahlberg S, Korhonen H, Lindfors E, Kangethe E (2016) Analysis of antibiotic residues in milk from smallholder farms in Kenya. Afr J Dairy Farming Milk Prod 3:152–158Google Scholar
  3. Becker-Algeri TA, Castagnaro D, de Bortoli K, de Souza C, Drunkler DA, Badiale-Furlong E (2016) Mycotoxins in bovine milk and dairy products: a review. J Food Sci 81:544–552CrossRefGoogle Scholar
  4. Bilandzic N, Varenina I, Kolanovic BS, Luburic DB, Benic M, Cvetnic L, Tankovic S, Cvetnic Z (2016) Monitoring of aflatoxin M1 in raw cow milk in Croatia during winter 2015. Mljekarstvo 66:81–85Google Scholar
  5. Boudra H, Barnouin J, Dragacci S, Morgavi DP (2007) Aflatoxin M1 and ochratoxin A in raw bulk milk from French dairy herds. J Dairy Sci 90:3197–3201CrossRefGoogle Scholar
  6. Denli M, Perez JF (2010) Ochratoxins in feed, a risk for animal and human health: control strategies. Toxins 2:1065–1077CrossRefGoogle Scholar
  7. EC (2002) European Commission. Comission regulation of 2002/657/EC, 12 August. Implementing council directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Official Journal of European Communities, L221/8–L221/36Google Scholar
  8. Economou V, Gousia P (2015) Agriculture and food animals as a source of antimicrobial resistant bacteria. Infect Drug Resist 8:49–61CrossRefGoogle Scholar
  9. Elizabeta DS, Zehra HM, Biljana SD, Pavle S, Risto U (2011) Screening of veterinary drug residues in milk from individual farms in Macedonia. Maced Vet Rev 34:5–13Google Scholar
  10. Elzupir AO, Makawi SZA, Elhussein AM (2009) Determination of aflatoxins and ochratoxin A in dairy cattle feed and milk in Wad Medani, Sudan. J Anim Vet Adv 8:2508–2511Google Scholar
  11. Gomes F, Henriques M (2015) Control of bovine mastitis: old and recent therapeutic approaches. Curr Microbiol 72:377–382CrossRefGoogle Scholar
  12. Hao H, Cheng G, Iqbal Z, Ai X, Hussain HI, Huang L, Dai M, Wang Y, Liu Z, Yuan Z (2014) Benefits and risks of antimicrobial use in food-producing animals. Front Microbiol 5:288.  https://doi.org/10.3389/fmicb.2014.00288 CrossRefPubMedPubMedCentralGoogle Scholar
  13. Iqbal SZ, Asi MR, Arino A (2011) Aflatoxin M1 contamination in cow and buffalo milk samples from the North West Frontier Province and Punjab Provinces of Pakistan. Food Addit Contam 4:282–288CrossRefGoogle Scholar
  14. Kebede G, Zenebe T, Disassa H, Tolosa T (2014) Review on detection of antimicrobial residues in raw bulk milk in dairy farms. Afr J Basic Appl Sci 6:87–97Google Scholar
  15. Kumar SG, Adithan C, Harish BN, Sujatha S, Roy G, Malini A (2013) Antimicrobial resistance in India: a review. J Nat Sci Biol Med 4:286–291CrossRefGoogle Scholar
  16. Madden JAJ, Plumier SF, Tang J, Garaiova I et al (2005) Effect of probiotics on preventing disruption of the intestinal microflora following antibiotic therapy: a double-blind, placebo-controlled pilot study. Int Immunopharmacol 5:1091–1097CrossRefGoogle Scholar
  17. Marshall BM, Levy SB (2011) Food animals and antimicrobials: impacts on human health. Clin Microbiol Rev 4:718–733CrossRefGoogle Scholar
  18. Moharana B, Venkatesh PK, Preetha SP, Selvasubramanian S (2015) Quantification of enrofloxacin residues in milk sample using RP-HPLC. World J Pharm Pharm Sci 4:1443–1450Google Scholar
  19. Mokhtari A, Hosseini B, Panah P (2013) Beta lactams and tetracyclines antibiotic residue detection in bulk tank milk in Iran. Iran J Public Health 42:447–448PubMedPubMedCentralGoogle Scholar
  20. Moudgil P, Bedi JS, Moudgil AD, Gill JPS, Aulakh RS (2017) Emerging issue of antibiotic resistance from food producing animals in India: perspective and legal framework. Food Rev Int 34:447–462CrossRefGoogle Scholar
  21. Nisha AR (2008) Antibiotics residues: a global health hazard. Vet World 1:375–377CrossRefGoogle Scholar
  22. Oliver SP, Murinda SE, Jayarao BM (2011) Impact of antibiotic use in adult dairy cows on antimicrobial resistance of veterinary and human pathogens: a comprehensive review. Foodborne Pathog Dis 8:337–355CrossRefGoogle Scholar
  23. Padol AR, Malapure CD, Domple VD, Kamdi BP (2015) Occurrence, public health implications and detection of antibacterial drug residues in cow milk. EWIJST 10:7–28Google Scholar
  24. Rahimi E, Bonyadian M, Rafei M, Kazemeini HR (2010) Occurrence of aflatoxin M1 in raw milk of five dairy species in Ahvaz, Iran. Food Chem Toxicol 48:129–131CrossRefGoogle Scholar
  25. RASFF (2011) Rapid alert system for food and feed. https://webgate.ec.europa.eu/rasff-window/portal. Accessed 18 Apr 2018
  26. Reddy KRN, Salleh B, Saad B, Abbas HK, Abel CA, Sheir WT (2010) An overview of mycotoxin contamination in foods and its implications for human health. J Toxicol Toxin Rev 29:3–26CrossRefGoogle Scholar
  27. Sandholm M, Kaartinen L, Pyorala S (2009) Bovine mastitis-why does antibiotics therapy not always work: an overview. J Vet Pharmacol Ther 13:248–260CrossRefGoogle Scholar
  28. Sefidgar S, Mirzae M, Assmar M, Naddaf S (2011) Aflatoxin M1 in pasteurized milk in Babol city, Mazanda-ran Province, Iran. Iran J Public Health 40:115–118PubMedPubMedCentralGoogle Scholar
  29. Shetty PH, Jespersen L (2009) Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontamination agents. Trends Food Sci Technol 17:48–55CrossRefGoogle Scholar
  30. Sudershan RV, Bhat RV (1995) A survey on veterinary drug use and residues in milk in Hyderabad. Food Addit Contam 12:645–650CrossRefGoogle Scholar
  31. Volkel I, Schroer-Merker E, Czerny CP (2011) The carry-over of mycotoxins in products of animal origin with special regard to its implications for the European food safety legislation. Food Nutr Sci 2:852–867Google Scholar
  32. Younis G, Ibrahim D, Awad A, El-Bardisy MM (2016) Determination of aflatoxin M1 and ochratoxinA in milk and dairy products in supermarkets located in Mansoura City, Egypt. J Anim Vet Ad 4:114–121CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Pallavi Moudgil
    • 1
    • 2
    Email author
  • Jasbir Singh Bedi
    • 1
  • Rabinder Singh Aulakh
    • 1
  • Jatinder Paul Singh Gill
    • 1
  1. 1.School of Public Health and ZoonosesGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia
  2. 2.Department of Veterinary Public Health and EpidemiologyLala Lajpat Rai University of Veterinary and Animal SciencesHisarIndia

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