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Journal of Food Science and Technology

, Volume 56, Issue 11, pp 4795–4798 | Cite as

Determination of 17β-estradiol in commercial pasteurized and sterilized milk samples in Mashhad, Iran

  • Bamdad Riahi-Zanjani
  • Mostafa Heidarzadegan
  • Hasan Badibostan
  • Gholamreza KarimiEmail author
Original Article
  • 60 Downloads

Abstract

Due to nutritional facts of milk in human life, the quality assessment of dairy products is of the utmost importance. The aim of the current study was to determine the 17β-estradiol level in commercial pasteurized and sterilized milk brands in Mashhad, Iran. In this regard, 160 samples including 80 pasteurized (40 high-fat and 40 low-fat) and 80 sterilized milk (40 high-fat and 40 low-fat) of widely used brands from different supermarkets were collected. The mean level of 17β-estradiol was 8.2 ± 0.59 pg/ml. The mean amount of estradiol was found to be 7.6 ± 0.47, 7.9 ± 0.45, 8.6 ± 0.63, and 8.9 ± 0.54 pg/ml for the low-fat pasteurized, low-fat sterilized, high-fat pasteurized and high-fat sterilized milk, respectively. There was no significant difference between the amount of estradiol in pasteurized and sterilized milk. As expected, the level of estradiol was statistically higher in high fat milks than that of low-fat milks. Considering the levels of 17β-estradiol measured here and the maximum permissible daily level of external estradiol entered to body through edible products recommended by EU and CAC (3.5 µg), at least in the short term, there will be no remarkable impact on the endocrine system. However, judging the long-term effects of using these products is not easy and simple at all, as cancers develop during a long period of time and has a multifactorial etiology.

Keywords

17β-Estradiol Pasteurized milk Sterilized milk 

Notes

Acknowledgements

The authors are thankful to the Vice Chancellor of Research, Mashhad University of Medical Sciences (Grand No. 941661) for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

References

  1. Codex Alimentarius Commission (2015) Maximum residue limits (MRLs) and risk management recommendations (RMRs) for residues of veterinary drugs in foods: CAC/MRL 2-2015. Updated as at the 37th session of the Codex Alimentarius Commission (July 2014)Google Scholar
  2. Fan Y, Yin YM, Jiang WB, Chen YP, Yang JW, Wu J, Xie MX (2014) Simultaneous determination of ten steroid hormones in animal origin food by matrix solid-phase dispersion and liquid chromatography–electrospray tandem mass spectrometry. Food Chem 142:170–177CrossRefGoogle Scholar
  3. Feng H, Ning L, Xiao-Li L (2016) Simultaneous determination of hexoestrol, diethylstilbestrol, estrone and 17-Beta-estradiol in feed by gas chromatography–mass spectrometry. J Northeast Agric Univ 23:44–49Google Scholar
  4. Gañán J, Gallego-Picó A, Garcinuño R, Fernández-Hernando P, Morante S, Sierra I, Durand JS (2012) Development of a molecularly imprinted polymer-matrix solid-phase dispersion method for selective determination of β-estradiol as anabolic growth promoter in goat milk. Anal Bioanal Chem 403:3025–3029CrossRefGoogle Scholar
  5. Hosseininia AR, Vahabzadeh M, Rashedinia M, Riahi-Zanjani B, Karimi G (2014) A survey of aflatoxins in sesame seeds imported into Khorasan Province, Iran. Mycotoxin Res 30:43–46CrossRefGoogle Scholar
  6. Macrina AL, Ott TL, Roberts RF, Kensinger RS (2012) Estrone and estrone sulfate concentrations in milk and milk fractions. J Acad Nutr Diet 112:1088–1093CrossRefGoogle Scholar
  7. Malekinejad H, Scherpenisse P, Bergwerff AA (2006) Naturally occurring estrogens in processed milk and in raw milk (from gestated cows). J Agric Food Chem 54:9785–9791CrossRefGoogle Scholar
  8. Mohammadi H, Shokrzadeh M, Aliabadi Z, Riahi-Zanjani B (2016) Occurrence of aflatoxin M1 in commercial pasteurized milk samples in Sari, Mazandaran province, Iran. Mycotoxin Res 32:85–87CrossRefGoogle Scholar
  9. Narendran R, Hacker RR, Smith VG, Lun A (1979) Estrogen and progesterone concentrations in bovine milk during the estrous cycle. Theriogenology 12:19–25CrossRefGoogle Scholar
  10. Pape-Zambito D, Magliaro A, Kensinger R (2007) Concentrations of 17β-estradiol in Holstein whole milk. J Dairy Sci 90:3308–3313CrossRefGoogle Scholar
  11. Pape-Zambito D, Roberts R, Kensinger R (2010) Estrone and 17β-estradiol concentrations in pasteurized-homogenized milk and commercial dairy products. J Dairy Sci 93:2533–2540CrossRefGoogle Scholar
  12. Rahimi E, Nilchian Z, Behzadnia A (2012) Presence of aflatoxin M1 in pasteurized and UHT milk commercialized in Shiraz, Khuzestan and Yazd, Iran. J Chem Health Risks 1:7–10Google Scholar
  13. Riahi-Zanjani B, Balali-Mood M (2013) Aflatoxin M1 contamination in commercial pasteurized milk from local markets in Fariman, Iran. Mycotoxin Res 29:271–274CrossRefGoogle Scholar
  14. Scippo ML, Van De Weerdt C, Willemsen P, François J-M, Rentier-Delrue F, Muller M, Martial JA, Maghuin-Rogister G (2002) Detection of illegal growth promoters in biological samples using receptor binding assays. Anal Chim Acta 473:135–141CrossRefGoogle Scholar
  15. Snoj T, Zuzek MC, Cebulj-Kadunc N, Majdic G (2018) Short communication: heat treatment and souring do not affect milk estrone and 17β-estradiol concentrations. J Dairy Sci 101:61–65CrossRefGoogle Scholar
  16. Varriale A, Pennacchio A, Pinto G, Oliviero G, D’Errico S, Majoli A, Scala A, Capo A, Pennacchio A, Di Giovanni S, Staiano M, D’Auria S (2015) A fluorescence polarization assay to detect steroid hormone traces in milk. J Agric Food Chem 63:9159–9164CrossRefGoogle Scholar
  17. Wang J, Cheng C, Yang Y (2015) Determination of estrogens in milk samples by magnetic-solid-phase extraction technique coupled with high-performance liquid chromatography. J Food Sci 80:C2655–C2661CrossRefGoogle Scholar
  18. Xiao L, Zhang Z, Wu C, Han L, Zhang H (2017) Molecularly imprinted polymer grafted paper-based method for the detection of 17β-estradiol. Food Chem 221:82–86CrossRefGoogle Scholar
  19. Zhang J, Wang L, Han Y (2013) Preparation of 17β-estradiol surface molecularly imprinted polymers and their application to the analysis of biological samples. J Sep Sci 36:3486–3492CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Bamdad Riahi-Zanjani
    • 1
  • Mostafa Heidarzadegan
    • 2
  • Hasan Badibostan
    • 2
  • Gholamreza Karimi
    • 2
    • 3
    Email author
  1. 1.Medical Toxicology Research CenterMashhad University of Medical SciencesMashhadIran
  2. 2.Department of Pharmacodynamics and Toxicology, School of PharmacyMashhad University of Medical SciencesMashhadIran
  3. 3.Pharmaceutical Research Center, Institute of Pharmaceutical TechnologyMashhad University of Medical SciencesMashhadIran

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