Organic solvent-free extraction of carotenoids from carrot bio-waste and its physico-chemical properties

  • Swati Tiwari
  • Neelam UpadhyayEmail author
  • Ashish Kumar Singh
  • Ganga Sahay Meena
  • Sumit Arora
Original Article


The bio-wastes (like peels, seeds, etc.) from food industry are rich source of bio-active components, but are poorly managed. In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefinery approach). Various combinations of time and temperature were used and final selection was made on the basis of maximum recovery of carotenoids. High shear disperser yielded maximum carotenoids (recovery 94.8 ± 0.08%). The total carotenoid content, antioxidant activity as ABTS, DDPH and FRAP and β-carotene of carotenoid rich extract from carrot pomace (CREP) were 82.66 ± 0.06 μg/g, 1596.04 ± 69.45 μg Trolox eq./ml, 380.21 ± 39.62 μg Trolox eq./ml, 941.20 ± 19.91 μM Trolox eq./ml, 78.37 μg/g, respectively were significantly higher (p < 0.05) when compared with the extracting medium. The L*, a* and b* values of CRE were 18.65 ± 0.037, 19.42 ± 0.21, 27.947 ± 0.65 and were significantly higher than extracting medium. The CRE could be used as a natural source of β-carotene and natural colorant for food applications.


Extraction Carrot pomace Flaxseed oil SEM β-Carotene HPLC 



The first author acknowledge the financial support received from Indian Council of Agricultural Research in the form of Junior Research Fellowship and Institutional Fellowship. Scanning Electron Microscopy was carried out at Punjab Agricultural University, Ludhiana.

Compliance with ethical standards

Conflict of interest

Authors do not have any conflict of interest.

Ethical approval

The study did not involve any animal study. Therefore, ethical approval was not required for the study.


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Dairy Technology DivisionICAR-National Dairy Research InstituteKarnalIndia
  2. 2.Dairy Chemistry DivisionICAR-National Dairy Research InstituteKarnalIndia

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