Chemical and physical changes during storage of differently packed biscuits formulated with sunflower oil

  • Federica Balestra
  • Vito Verardo
  • Silvia TappiEmail author
  • Maria Fiorenza Caboni
  • Marco Dalla Rosa
  • Santina Romani
Original Article


Various innovative materials are already present on the market, but there is scarce knowledge on their performances when used in real food system. In this study, two innovative packaging materials were investigated for the evaluation during storage of biscuits formulated with sunflower oil and compared to a traditional one. To this aim, three different flexible and composite film containing a metalized plastic layer and a paper layer were used. The control included orientated polypropylene (OPP), while the innovative materials contained poly-lactic acid or OPP with a pro-oxidant additive, ethylene vinyl acetate. The physical (moisture, water activity, hardness/crispness) and chemical (peroxide value, conjugated dienes and trienes, and hexanal and nonanal formation) changes of biscuits were monitored during accelerated storage (35 °C and 50% of relative humidity for 105 days). Packaging materials did not have significant impact on textural quality of biscuits. Instead remarkable differences were observed during storage in the evolution of different lipid oxidation compounds, moisture content and water activity among differently packed biscuits. A new ecofriendly packaging showed the best performances in terms of physico-chemical quality of biscuits. The obtained results provide useful information for industrial application.


Biscuits Packaging Quality Lipid oxidation Structural properties 



Vito Verardo thanks Spanish Ministry of Economy and Competitiveness (MINECO) for his “Ramon y Cajal” contract.


  1. AOAC (1980) Official methods of analysis. In: Horwitz E (ed). AOAC, Washington DCGoogle Scholar
  2. Calligaris S, Manzocco L, Kravina G, Nicoli MC (2007) Shelf-life modelling of bakery products by using oxidation indices. J Agric Food Chem 55(5):2004–2009CrossRefGoogle Scholar
  3. Calligaris S, Pieve SD, Kravina G, Manzocco L, Nicoli CM (2008) Shelf life prediction of bread sticks using oxidation indices: a validation study. J Food Sci 73(2):E51–E56CrossRefGoogle Scholar
  4. Choe E, Min DB (2006) Mechanisms and factors for edible oil oxidation. Comp Rev Food Sci Food Saf 5(4):169–186CrossRefGoogle Scholar
  5. European Community (1991) Regulation EC No 2568/1991 Official Journal of the European Community, L248Google Scholar
  6. Farris S, Piergiovanni L (2009) Optimization of manufacture of almond paste cookies using response surface methodology. J Food Proc Eng 32(1):64–87CrossRefGoogle Scholar
  7. Frankel EN (1991) Recent advances in lipid oxidation. J Sci Food Agric 54(4):495–511CrossRefGoogle Scholar
  8. Galić K, Ćurić D, Gabrić D (2009) Shelf life of packaged bakery goods—a review. Crit Rev Food Sci Nutr 49(5):405–426CrossRefGoogle Scholar
  9. International Union of Pure and Applied Chemistry (IUPAC) (1979) Standard methods for the analysis of oils, fats and derivatives. In: Paquot C (ed) IUPAC method II. D. 23, 6th edn. Pergamon Press, OxfordGoogle Scholar
  10. Kim JY, Kim MJ, Lee J (2014) Role of moisture on the lipid oxidation determined by D2O in a linoleic acid model system. Food Chem 146:134–140CrossRefGoogle Scholar
  11. Lu LX, Xu F (2009) Effect of light-barrier property of packaging film on the photo-oxidation and shelf life of cookies based on accelerated tests. Packag Technol Sci 22(2):107–113CrossRefGoogle Scholar
  12. Marzocchi S, Pasini F, Verardo V, Ciemniewska-Żytkiewicz H, Caboni MF, Romani S (2017) Effects of different roasting conditions onphysical-chemical properties of Polish hazelnuts (Corylus avellana L. var.Kataloński). LWT 77:440–448CrossRefGoogle Scholar
  13. Mandić AI, Sedej IJ, Sakač MB, Mišan AČ (2013) Static headspace gas chromatographic method for aldehyde determination in crackers. Food Anal Methods 6(1):61–68CrossRefGoogle Scholar
  14. Mildner-Szkudlarz S, Zawirska-Wojtasiak R, Obuchowski W, Gośliński M (2009) Evaluation of antioxidant activity of green tea extract and its effect on the biscuits lipid fraction oxidative stability. J Food Sci 74(8):S362–S370CrossRefGoogle Scholar
  15. Park JW, Kim JY, Kim MJ, Lee J (2014) Evaluation of oxygen-limitation on lipid oxidation and moisture content in corn oil at elevated temperature. J Am Oil Chem Soc 91(3):439–444CrossRefGoogle Scholar
  16. Pauly A, Pareyt B, Lambrecht MA, Fierens E, Delcour JA (2013) Flour from wheat cultivars of varying hardness produces semi-sweet biscuits with varying textural and structural properties. LWT-Food Sci Technol 53(2):452–457CrossRefGoogle Scholar
  17. Piergiovanni L, Limbo S (2004) The protective effect of film metallization against oxidative deterioration and discoloration of sensitive foods. Packag Technol Sci 17(3):155–164CrossRefGoogle Scholar
  18. Purcaro G, Moret S, Conte LS (2008) HS–SPME–GC applied to rancidity assessment in bakery foods. Eur Food Res Technol 227(1):1CrossRefGoogle Scholar
  19. Robertson GL (2006) Modified atmosphere packaging. In: Food packaging—principles and practice, Second edn. CRC Press, Boca RatonGoogle Scholar
  20. Romani S, Balestra F, Angioloni A, Rocculi P, Rosa MD (2012) Physico-chemical and electronic nose measurements on the study of biscuit baking kinetics. Ital J Food Sci 24(1):32–40Google Scholar
  21. Romani S, Rocculi P, Tappi S, Dalla Rosa M (2016) Moisture adsorption behaviour of biscuit during storage investigated by using a new Dynamic Dewpoint method. Food Chem 195:97–103CrossRefGoogle Scholar
  22. Romani S, Rodriguez-Estrada MT (2016) Bakery products and electronic nose. In: Electronic noses and tongues in food science, pp 39–47.
  23. Romani S, Tappi S, Balestra F, Rodriguez Estrada MT, Siracusa V, Rocculi P, Dalla Rosa M (2015) Effect of different new packaging materials on biscuit quality during accelerated storage. J Sci Food Agric 95(8):1736–1746CrossRefGoogle Scholar
  24. Siracusa V, Rocculi P, Romani S, Dalla Rosa M (2008) Biodegradable polymers for food packaging: a review. Trends Food Sci Technol 19(12):634–643CrossRefGoogle Scholar
  25. Smith JP, Daifas DP, El-Khoury W, Koukoutsis J, El-Khoury A (2004) Shelf life and safety concerns of bakery products—a review. Crit Rev Food Sci 44(1):19–55CrossRefGoogle Scholar
  26. Sudha ML, Srivastava AK, Vetrimani R, Leelavathi K (2007) Fat replacement in soft dough biscuits: its implications on dough rheology and biscuit quality. J Food Eng 80(3):922–930CrossRefGoogle Scholar
  27. Tyagi SK, Manikantan MR, Oberoi HS, Kaur G (2007) Effect of mustard flour incorporation on nutritional, textural and organoleptic characteristics of biscuits. J Food Eng 80(4):1043–1050CrossRefGoogle Scholar
  28. Verardo V, Riciputi Y, Messia MC, Vallicelli M, Falasca L, Marconi E, Caboni MF (2011) Dietary fiber and flavan-3-ols in shortbread biscuits enriched with barley flours co-products. Int J Food Sci Nutr 62(3):262–269CrossRefGoogle Scholar
  29. Verardo V, Riciputi Y, Trivisonno MC, Marconi E, Caboni MF (2010) Effect of the addition of air-classified barley flours on the lipid stability of bakery products. Eur Food Res Technol 231(2):309–319CrossRefGoogle Scholar
  30. Vichi S, Pizzale L, Conte LS, Buxaderas S, López-Tamames E (2003) Solid-phase microextraction in the analysis of virgin olive oil volatile fraction: modifications induced by oxidation and suitable markers of oxidative status. J Agric Food Chem 51(22):6564–6571CrossRefGoogle Scholar
  31. Vitali D, Dragojević IV, Šebečić B (2009) Effects of incorporation of integral raw materials and dietary fibre on the selected nutritional and functional properties of biscuits. Food Chem 114(4):1462–1469CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Federica Balestra
    • 1
  • Vito Verardo
    • 2
    • 3
  • Silvia Tappi
    • 4
    Email author
  • Maria Fiorenza Caboni
    • 1
    • 4
  • Marco Dalla Rosa
    • 1
    • 4
  • Santina Romani
    • 1
    • 4
  1. 1.Department of Agri-Food Science and TechnologyAlma Mater Studiorum, Università di BolognaCesenaItaly
  2. 2.Department of Nutrition and Food ScienceUniversity of GranadaGranadaSpain
  3. 3.Institute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research CentreUniversity of GranadaGranadaSpain
  4. 4.Interdepartmental Centre for Agri-Food Industrial ResearchAlma Mater Studiorum, Università di BolognaCesenaItaly

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