Preparative scale extraction of mangiferin and lupeol from mango (Mangifera indica L.) leaves and bark by different extraction methods

  • Jenit Margarita Lerma-Torres
  • Arturo Navarro-Ocaña
  • Montserrat Calderón-Santoyo
  • Liliana Hernández-Vázquez
  • Gabriela Ruiz-Montañez
  • Juan Arturo Ragazzo-SánchezEmail author
Original Article


High biological value compounds are very important in the food and pharmaceutical sectors. The leading research interests are seeking efficient methods for extracting these substances. The objective of this study was to evaluate different extraction methods to obtain mangiferin and lupeol at preparative scale from leaves and bark of mango tree varieties Ataulfo and Autochthonous from Nayarit, Mexico. Four extraction techniques were evaluated such as maceration, Soxhlet, sonication (UAE) and microwave (MAE). Sonication gave the highest concentration of mangiferin and lupeol, demonstrating that extraction assisted by ultrasound could be an effective alternative to conventional extraction techniques because it is a low cost, simple and reliable process. Finally, mangiferin and lupeol were obtained at preparative scale with a higher concentration of bioactive compounds, 1.45 g 100 g−1 y 0.92 mg 100 g−1 sample on (d.b.), respectively. The barks from Ataulfo and Autochthonous mango trees turned out to be favourable sources for obtaining mangiferin and lupeol.


Mango leaves Mango bark Assisted extraction Mangiferin Lupeol Preparative scale 



  1. Ajila CM, Bhat SG, Prasada Rao UJS (2007) Valuable components of raw and ripe peels from two Indian mango varieties. Food Chem 102:1006–1011. CrossRefGoogle Scholar
  2. Ashokkumar M, Mason TJ (2007) Sonochemistry. Wiley, New YorkGoogle Scholar
  3. Aspé E, Fernández K (2011) The effect of different extraction techniques on extraction yield, total phenolic, and anti-radical capacity of extracts from Pinus radiata Bark. Ind Crops Prod 34:838–844. CrossRefGoogle Scholar
  4. Barreto JC, Trevisan MTS, Hull WE et al (2008) Characterization and quantitation of polyphenolic compounds in bark, kernel, leaves, and peel of mango (Mangifera indica L.). J Agric Food Chem 56:5599–5610. CrossRefGoogle Scholar
  5. Berardini N, Fezer R, Conrad J et al (2005) Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O- and xanthone C-glycosides, anthocyanins, and pectin. J Agric Food Chem 53:1563–1570. CrossRefGoogle Scholar
  6. Bucar F, Wube A, Schmid M (2013) Natural product isolation-how to get from biological material to pure compounds. Nat Prod Rep 30:525–545CrossRefGoogle Scholar
  7. Chemat F, Rombaut N, Sicaire AG et al (2017) Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem 34:540–560CrossRefGoogle Scholar
  8. Djilani A, Legseir B, Soulimani R et al (2006) New extraction technique for alkaloids. J Braz Chem Soc 17:518–520. CrossRefGoogle Scholar
  9. Feng H, Barbosa-Cánovas GV, Weiss J (2011) Ultrasound technologies for food an bioprocessing. Springer, New YorkCrossRefGoogle Scholar
  10. Gao M, Liu CZ (2005) Comparison of techniques for the extraction of flavonoids from cultured cells of Saussurea medusa Maxim. World J Microbiol Biotechnol 21:1461–1463. CrossRefGoogle Scholar
  11. Hemwimol S, Pavasant P, Shotipruk A (2006) Ultrasound-assisted extraction of anthraquinones from roots of Morinda citrifolia. Ultrason Sonochem 13:543–548. CrossRefGoogle Scholar
  12. Hernandez P, Rodriguez PC, Delgado R, Walczak H (2007) Protective effect of Mangifera indica L. polyphenols on human T lymphocytes against activation-induced cell death. Pharmacol Res 55:167–173. CrossRefGoogle Scholar
  13. Jagetia GC, Venkatesha VA (2006) Mangiferin protects human peripheral blood lymphocytes against γ-radiation-induced DNA strand breaks: a fluorescence analysis of DNA unwinding assay. Nutr Res 26:303–311. CrossRefGoogle Scholar
  14. Murtaza I, Saleem M, Adhami VM et al (2009) Suppression of cFLIP by lupeol, a dietary triterpene, is sufficient to overcome resistance to TRAIL-mediated apoptosis in chemoresistant human pancreatic cancer cells. Cancer Res 69:1156–1165. CrossRefGoogle Scholar
  15. Núñez Sellés AJ, Vélez Castro HT, Agüero-Agüero J et al (2002) Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. J Agric Food Chem 50:762–766. CrossRefGoogle Scholar
  16. Rajendran P, Ekambaram G, Sakthisekaran D (2008) Cytoprotective effect of mangiferin on benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice. Basic Clin Pharmacol Toxicol 103:137–142. CrossRefGoogle Scholar
  17. Ribeiro SMR, Barbosa LCA, Queiroz JH et al (2008) Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem 110:620–626. CrossRefGoogle Scholar
  18. Rodríguez J, Di Pierro D, Gioia M et al (2006) Effects of a natural extract from Mangifera indica L, and its active compound, mangiferin, on energy state and lipid peroxidation of red blood cells. Biochim Biophys Acta: Gen Subject 1760:1333–1342. CrossRefGoogle Scholar
  19. Ruiz-Montañez G, Ragazzo-Sánchez JA, Calderón-Santoyo M et al (2014) Evaluation of extraction methods for preparative scale obtention of mangiferin and lupeol from mango peels (Mangifera indica L.). Food Chem 159:267–272. CrossRefGoogle Scholar
  20. Saha S, Sadhukhan P, Sinha K et al (2016) Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3 K induced Akt and Nrf-2 mediated signaling pathways. Biochem Biophys Rep 5:313–327. Google Scholar
  21. Saleem M (2009) Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett 285:109–115CrossRefGoogle Scholar
  22. Sudhahar V, Ashok Kumar S, Varalakshmi P, Sujatha V (2008) Protective effect of lupeol and lupeol linoleate in hypercholesterolemia associated renal damage. Mol Cell Biochem 317:11–20. CrossRefGoogle Scholar
  23. Tiwari BK, Muthukumarappan K, O’Donnell CP, Cullen PJ (2008) Effects of sonication on the kinetics of orange juice quality parameters. J Agric Food Chem 56:2423–2428. CrossRefGoogle Scholar
  24. Wang L, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Technol 17:300–312CrossRefGoogle Scholar
  25. Wilkinson AS, Monteith GR, Shaw PN et al (2008) Effects of the mango components mangiferin and quercetin and the putative mangiferin metabolite norathyriol on the transactivation of peroxisome proliterator-activated receptor isoforms. J Agric Food Chem 56:3037–3042. CrossRefGoogle Scholar
  26. Zhao Z, Dong L, Wu Y, Lin F (2011) Preliminary separation and purification of rutin and quercetin from Euonymus alatus (Thunb.) Siebold extracts by macroporous resins. Food Bioprod Process 89:266–272. CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Jenit Margarita Lerma-Torres
    • 1
  • Arturo Navarro-Ocaña
    • 2
  • Montserrat Calderón-Santoyo
    • 1
  • Liliana Hernández-Vázquez
    • 3
  • Gabriela Ruiz-Montañez
    • 1
  • Juan Arturo Ragazzo-Sánchez
    • 1
    Email author
  1. 1.Laboratorio Integral de Investigación en AlimentosTecnológico Nacional de México/Instituto Tecnológico de TepicTepicMexico
  2. 2.Facultad de Química, Circuito InteriorUniversidad Nacional Autónoma de MéxicoCoyoacánMexico
  3. 3.Departamento de Sistemas BiológicosUniversidad Autónoma MetropolitanaMexicoMexico

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