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Rapid determination of antioxidant molecules in volatiles of rose tea by gas chromatography–mass spectrometry combined with DPPH reaction

  • Hao Qin
  • Bao-cai Li
  • Wei-feng Dai
  • Cheng Xiang
  • Yi Qin
  • Shi-yun Jiao
  • Mi ZhangEmail author
Original Article
  • 3 Downloads

Abstract

Volatiles have been regarded as active substances in many foods, whose chemicals can be analyzed by GC–MS qualitatively and quantitatively. However, the activities of volatiles are often studied as a whole, and it has no an effective method to determine that which molecule is active in volatiles by far. In order to identify the antioxidant molecules in volatiles, a rapid determination method was developed by GC–FID/MS combined with DPPH radical reaction in this study. Three antioxidant molecules were identified and validated among 20 components in rose tea infusion. Their activity validation and the methodological evaluation indicated this method could be used for distinguishing antioxidant molecules in volatiles rapidly and effectively.

Keywords

Antioxidant Volatile GC–MS Rose tea DPPH reaction 

Notes

Acknowledgements

The research work was financially supported by the National Natural Science Foundation of China (No. 21466018).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

References

  1. Ahmad M, Baba WN, Gani A, Wani TA, GaniA Masoodi FA (2015) Effect of extraction time on antioxidants and bioactive volatile components of green tea (Camellia sinensis), using GC/MS. Cog Food Agric 1:1106387Google Scholar
  2. Ani V, Varadaraj MC, Naidu KA (2006) Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.). Eur Food Res Technol 224:109–115CrossRefGoogle Scholar
  3. Arenas DRM, Acevedo AM, Méndez LYV, Kouznetsov VV (2011) Scavenger activity evaluation of the clove bud essential oil (eugenia caryophyllus) and eugenol derivatives employing ABTS decolorization. Sci Pharm 79:779–791CrossRefGoogle Scholar
  4. Belviso S, Ghirardello D, Giordano M, Ribeiro GS, Alves JS, Parodi S, Risso S, Zeppa G (2013) Phenolic composition, antioxidant capacity and volatile compounds of licuri (Syagrus coronata (Martius) Beccari) fruits as affected by the traditional roasting process. Food Res Int 51:39–45CrossRefGoogle Scholar
  5. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30CrossRefGoogle Scholar
  6. Čechovská L, Cejpek K, Konečnŷ M, Velíšek J (2011) On the role of 2,3-dihydro-3,5-dihydroxy-6-methyl-(4H)-pyran-4-one in antioxidant capacity of prunes. Eur Food Res Technol 233:367–376CrossRefGoogle Scholar
  7. Foti MC, Daquino C, Geraci C (2004) Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH· radical in alcoholic solutions. J Org Chem 69:2309–2314CrossRefGoogle Scholar
  8. Gao Q, Sha Y, Wu D, Liu B, Chen C, Fang D (2012) Analysis of the volatile components emitted from cut tobacco processing by gas chromatography/mass spectrometry thermal desorption system. Talanta 101:198–202CrossRefGoogle Scholar
  9. Gülçin İ (2012) Antioxidant activity of food constituents: an overview. Arch Toxicol 86:345–391CrossRefGoogle Scholar
  10. Harman D (1956) Aging: a theory based on free radical and radiation chemistry. J Gerontol 11:298–300CrossRefGoogle Scholar
  11. Kosar M, Demirci B, Goger F, Kara I, Baser KHC (2017) Volatile composition, antioxidant activity, and antioxidant components in saffron cultivated in Turkey. Int J Food Prop 20:S746–S754CrossRefGoogle Scholar
  12. Li F, Zhang LD, Li BC, Yang J, Yu H, Wan JB, Wang JB, Li P (2012) Screening of free radical scavengers from Erigeron breviscapus using on-line HPLC-ABTS/DPPH based assay and mass spectrometer detection. Free Radic Res 46:286–294CrossRefGoogle Scholar
  13. Lopez P, Sisseren MV, Marco SD, Jekel A, Nijs M, Mol HGJ (2015) A straightforward method to determine flavouring substances in food by GC–MS. Food Chem 174:407–416CrossRefGoogle Scholar
  14. Lorenzo JM, Munekata PES, Gómez B, Barba FJ, Mora L, Pérez-Santaescolásticaa C, Toldrá F (2018) Bioactive peptides as natural antioxidants in food products—a review. Trends Food Sci Technol 79:136–147CrossRefGoogle Scholar
  15. Meersche TVD, Pamel EV, Poucke CV, Herman L, Heyndrickx M, Rasschaert G, Daeseleire E (2015) Development, validation and application of an ultra high performance liquid chromatographic-tandem mass spectrometric method for the simultaneous detection and quantification of five different classes of veterinary antibiotics in swine manure. J Chromatogr A 1429:248–257CrossRefGoogle Scholar
  16. Mohamd RH, Elbastawesy AM, Abdelmonem MG, Noor AM, Almehdar HA, Sharawy SM, EI-Merzabani MM (2011) Antioxidant and anticarcinogenic effects of methanolic extract and volatile oil of fennel seeds (Foeniculum vulgare). J Med Food 14:986–1001CrossRefGoogle Scholar
  17. Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant. Songklanakarin J Sci Technol 26:211–219Google Scholar
  18. Park EJ, Kim Y, Kim J (2000) Acylated flavonol glycosides from the flower of Inula britannica. J Nat Prod 63:34–36CrossRefGoogle Scholar
  19. Rio DD, Stewart AJ, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovas 15:316–328CrossRefGoogle Scholar
  20. Sarbanha S, Masoomi F, Kamalinejad M, Yassa N (2011) Chemical composition and antioxidant activity of Salvia virgata Jacq. and S. verticillata L. volatile oils from iran. Planta Med 77:207–223CrossRefGoogle Scholar
  21. Ud-Daula AFMS, Demirci F, Salim KA, Demirci B, Lim LBL, Baser KHC, Ahmad N (2016) Chemical composition, antioxidant and antimicrobial activities of essential oils from leaves, aerial stems, basal stems, and rhizomes of Etlingera fimbriobracteata (K. Schum.) R.M. Sm. Ind Crop Prod 84:189–198CrossRefGoogle Scholar
  22. Umezu T, Ito H, Nagano K, Yamakoshi M, Oouchi H, Sakaniwa M, Morita M (2002) Anticonflict effects of rose oil and identification of its active constituents. Life Sci 72:91–102CrossRefGoogle Scholar
  23. Villaño D, Fernándezpachón MS, Moyá ML, Troncoso AM, Garcíaparrilla MC (2007) Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 71:230–235CrossRefGoogle Scholar
  24. Wang YN (2009) The study on the secondary metabolites form Y19-07 of marine. Dissertation, Shengyang Pharmaceutical UniversityGoogle Scholar
  25. Wang DD, Li N, Liu SK, Qi L, Pan BY, Xu J (2012) Gas chromatographic-mass spectroscopic analysis of the chemical components in ethanol and water extract of rumex seeds. Food Res Dev 33:129–132Google Scholar
  26. Wei A, Shibamoto T (2007) Antioxidant activities and volatile constituents of various essential oils. J Agric Food Chem 55:1737–1742CrossRefGoogle Scholar
  27. Wungsintaweekul J, Putalun W, Sitthithaworn W, Pfeifhofer HW, Brantner AH (2007) Chemical composition of volatile oils and evaluation of antioxidant and antimicrobial activities of ethnopharmacologically selected thai medicinal plants. Planta Med 73:633–634CrossRefGoogle Scholar
  28. Xue DY, Li ZL (1989) Compassion of chemical components of four kinds of rose oil produced in China. Nat Prod Res Dev 1:36–41Google Scholar
  29. Yin DD, Yuan RY, Wu Q, Li SS, Shao S, Xu YJ, Hao XH, Wang LS (2015) Assessment of flavonoids and volatile compounds in tea infusions of water lily flowers and their antioxidant activities. Food Chem 187:20–28CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingChina

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