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Culture fermentation of Lactobacillus in traditional pickled gherkins: Microbial development, chemical, biogenic amine and metabolite analysis

  • Yusuf AlanEmail author
Original Article
  • 18 Downloads

Abstract

Fermented cucumber pickles are the lactic acid fermentation products formed through the influence of microorganisms present in the environment. This study investigated the impacts of starter cultures, namely, Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus paraplantarum, typically utilized for the fermentation of traditional pickled gherkins, on fermentation process. The chemical (pH, total acidity and salt) and microbiological (total mesophilic aerobic bacteria, lactic acid bacteria and yeast-mould) changes were observed against the control sample during fermentation process. Moreover, the amounts of biogenic amines (BAs) and metabolites formed as a consequence of fermentation were determined using HPLC. It was found that the chemical analyses provided similar results for all the samples. The amount of total mesophilic aerobic bacteria and yeast-mould colonies in pickle sample containing L. plantarum 49 strain appeared to reduce significantly. The amount of BAs was the lowest for the pickle samples where L. plantarum strains were added. The amount of BAs was below the toxic value that could affect human health. More BAs were synthesized as the fermentation period increased. Lactate was seen to exist in the samples when pyruvate was present, and acetoin was converted into 2.3-butanediol during the fermentation period. It was concluded that the pickle sample for which L. plantarum 49 strain was used displayed a better fermentation profile (i.e., metabolite and biogenic amines) than the remaining samples. Producing a more delicious and reliable product using such characteristics of L. plantarum strains in pickled gherkins is believed to significantly contribute to the food industry.

Keywords

Pickled gherkins Lactobacillus Biogenic amines Metabolite 

Notes

Acknowledgements

I thank Dr. Ahmet Savcı, Dr. Enver Fehim Kocpınar and Academician Neslihan Yıldız for their precious contributions to the present study.

References

  1. Aktan N, Kalkan H (2000) Şarap teknolojisi. Kavaklıdere Eğitim Yayınları, AnkaraGoogle Scholar
  2. Aktan N, Yücel U, Kalkan H (1998) Turşu teknolojisi. Ege Üniversitesi Ege Meslek Yüksek Okulu Yayınları, İzmirGoogle Scholar
  3. Alan D (2015) Doğal turşulardan Lactobacillus paraplantarum ve Lactobacillus pentosus suşlarının moleküler tanımlanması ve plazmid içeriklerinin belirlenmesi. K.S.Ü, Fen Bilimleri Enstitüsü, KahramanmaraşGoogle Scholar
  4. Alan Y, Topalcengiz Z, Dığrak M (2018) Biogenic amine and fermentation metabolite production assessments of Lactobacillus plantarum isolates for naturally fermented pickles. LWT Food Sci Technol 98:322–328CrossRefGoogle Scholar
  5. Aryal S (2016) Spread plate technique- principle, procedure and uses. Accessed from: http://www.microbiologyinfo.com/spread-plate-techniqueprinciple-procedure-and-uses. Accessed 5 May 2017
  6. Doeun D, Davaatsere M, Chung MS (2017) Biogenic amines in foods. Food Sci Biotechnol 26:1463–1474CrossRefGoogle Scholar
  7. Fernández-Diez MJ, Castro RR, Garrido FA, Heredia MA et al (1985) Biotecnologia de la aceituna de mesa. Instituto de la Grasa y sus Derivados, SevillaGoogle Scholar
  8. Franco W, Perez-Dıaz IM, Johanningsmeier SD, McFeeters RF (2012) Characteristics of spoilage-associated secondary cucumber fermentation. J Appl Environ Microbiol 78:1273–1284CrossRefGoogle Scholar
  9. Gaspar P, Carvalho AL, Vinga S, Santos H, Neves AR (2013) From physiology to systems metabolic engineering for the production of biochemicals by lactic acid bacteria. Biotechnol Adv 31:764–788CrossRefGoogle Scholar
  10. Halasz A, Barath A, Sımon-Sarkadı L, Holzapfel W (1994) Biogenic amines and their production by microorganisms in food. Trends Food Sci Technol 5:42–49CrossRefGoogle Scholar
  11. Herreros MA, Sandoval H, González L, Castro JM, Fresno JM, Tornadijo ME (2005) Antimicrobial activity and antibiotic resistance of lactic acid bacteria isolated from Armada cheese (a Spanish goats’ milk cheese). Food Microbiol 22:455–459CrossRefGoogle Scholar
  12. Hutkins RW (2006) Microbiology and technology of fermented foods. IFT Press, Blackwell Publishing Professional, LowaCrossRefGoogle Scholar
  13. La Gioia F, Rizzotti L, Rossi F, Gardini F, Tabanelli G, Torriani S (2011) Identification of a tyrosine decarboxylase gene (tdcA) in Streptococcus thermophilus 1TT45 and analysis of its expression and tyramine production in milk. Appl Environ Microbiol 77:1140–1144CrossRefGoogle Scholar
  14. Lee YC, Kung HF, Huang YL, Wu CH, Huang YR, Tsai YH (2016) Reduction of biogenic amines during miso fermentation by Lactobacillus plantarum as a starter culture. J Food Prot 9:1468–1646Google Scholar
  15. Liao M, Wu ZY, Yu GH, Zhang WX (2017) Improving the quality of Sichuan pickle by adding a traditional Chinese medicinal herb Lycium barbarum in its fermentation. Int J Food Sci Technol 52:936–943CrossRefGoogle Scholar
  16. Lind H, Jonsson H, Schnürer J (2005) Antifungal effect of dairy propionibacteria contribution of organic acids. Int J Food Microbiol 98:157–165CrossRefGoogle Scholar
  17. Liu S, Nichols NN, Dien BS, Cotta MA (2006) Metabolic engineering of a Lactobacillus plantarum double ldh knockout strain for enhanced ethanol production. J Ind Microbiol Biotechnol 33:1–7CrossRefGoogle Scholar
  18. Liu Q, Wu J, Lim ZY, Aggarwal A, Yang H, Wang S (2017) Evaluation of the metabolic response of Escherichia coli to electrolysed water by 1H NMR spectroscopy. LWT Food Sci Technol 79:428–436CrossRefGoogle Scholar
  19. Lu Z, Breidt F, Fleming HP, Altermann E, Klaenhammer TR (2003) Isolation and characterization of a Lactobacillus plantarum bacteriophage, øJL-1, from a cucumber fermentation. Int J Food Microbiol 84:225–235CrossRefGoogle Scholar
  20. Nilchian Z, Rahimi E, Razavi SH, Shahraki MM (2016) Isolation and identification of L. plantarum from Iranian fermented cucumbers by conventional culture and PCR methods. J Food Biosci Technol 6:69–76Google Scholar
  21. Nout MJR (1994) Fermented foods and food safety. Food Res Int 27:291–298CrossRefGoogle Scholar
  22. Panghal A, Virkar K, Kumar V, Dhull SB, Gat Y, Chhikara N (2017) Development of probiotic beetroot drink. Curr Res Nutr Food Sci 5:257–262CrossRefGoogle Scholar
  23. Panghal A, Janghu S, Virkar K, Gat Y, Kumar V, Chhikara N (2018) Potential non-dairy probiotic products—a healthy approach. Food Biosci 21:80–89CrossRefGoogle Scholar
  24. Pérez-Díaz IM, Breidt F, Buescher RW, Arroyo-Lopez FN et al (2014) Fermented and acidified vegetables. Compendium of methods for the microbiological examination of foods. APHA Press, Washington, DCGoogle Scholar
  25. Plenghvidhya V, Breidt F, Lu Z, Fleming HP (2007) DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations. Appl Environ Microbiol 73:7697–7702CrossRefGoogle Scholar
  26. Rabie MA, Siliha H, El-Saidy S, El-Badawy AA, Malcata FX (2011) Reduced biogenic amines contents is sauerkraut via addition of selected lactic acid bacteria. Food Chem 129:1778–1782CrossRefGoogle Scholar
  27. Rodríguez-Gomez F, Bautista-Gallego J, Romero-Gil V, Arroyo-Lopez NF, Garrido- Fernández A, García-García P (2012) Effects of salt mixtures on Spanish green table olive fermentation performance. LWT Food Sci Technol 46:56–63CrossRefGoogle Scholar
  28. Sáez GD, Flomenbaum L, Zárate G (2018) Lactic acid bacteria from argentinean fermented foods: isolation and characterization for their potential use as starters for fermentation of vegetables. Food Technol Biotechnol 56:398–410CrossRefGoogle Scholar
  29. Sahu L, Pand SK, Paramithiotis S, Zdolec N, Ray RC (2016) Biogenic amines in fermented foods: Overview. In: Montet D, Ray RC (eds) Fermented foods Part 1: Biochemistry and biotechnology. Taylor & Francis Group, LLC, CRC Press, Florida, pp 303–317Google Scholar
  30. Schnürer J, Magnusson J (2005) Antifungal lactic acid bacteria as biopreservatives. Trends Food Sci Technol 16:70–78CrossRefGoogle Scholar
  31. Solem C, Dehli T, Jensen PR (2013) Rewiring Lactococcus lactis for ethanol production. Appl Environ Microbiol 79:2512–2520CrossRefGoogle Scholar
  32. Tamang J, Tamang B, Schillinger U, Guigas C, Holzapfel WH (2009) Functional properties of lactic acid bacteria isolated from ethnic fermented vegetables of the Himalayas. Int J Food Microbiol 135:28–33CrossRefGoogle Scholar
  33. Tsuji A, Okada S, Hols P, Satoha E (2013) Metabolic engineering of Lactobacillus plantarum for succinic acid production through activation of the reductive branch of the tricarboxylic acid cycle. Enzym Microb Technol 53:97–103CrossRefGoogle Scholar
  34. Williams AG, Noble J, Banks JM (2001) Catabolism of amino acids by lactic acid bacteria isolated from cheddar cheese. Int Dairy J 11:203–215CrossRefGoogle Scholar
  35. Wouters D, Bernaert N, Conjaerts W, Droogenbroeck BV, Loose MD, Vuyst LD (2013) Species diversity, community dynamics, and metabolite kinetics of spontaneous leek fermentations. Food Microbiol 33:185–196CrossRefGoogle Scholar
  36. Xiong T, Li J, Liang F, Wang Y, Guan Q, Xie M (2016) Effects of salt concentration on Chinese sauerkraut fermentation. LWT Food Sci Technol 69:169–174CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Department of Primary Education, Faculty of EducationMuş Alparslan UniversityMuşTurkey

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