Targeted phenolic profiling of Sauvignon blanc and Shiraz grapes grown in two regions of India by liquid chromatography-tandem mass spectrometry
The phenolic compounds play an important role in production of quality grapes and wines. The current investigation focused on optimization of an extraction method for targeted analysis of 33 phenolic compounds in grapes by liquid chromatography tandem mass spectrometry (LC–MS/MS). The optimized method was successfully used for phenolic profiling of two wine grape varieties, Sauvignon blanc (white) and Shiraz (red) originated from Pune and Nasik regions of Maharashtra State, India. The optimized sample preparation procedure involved liquid–liquid extraction with acidified methanol by vortexing for 2 min followed by analysis on LC–MS/MS. The limit of quantification of the targeted compounds was in the range of 29 to 411 µg/L. The results indicated that skin of both varieties contained the highest amount of flavonols (69.47 ± 14.74 mg/kg in Sauvignon blanc and 129.47 ± 10.05 mg/kg in Shiraz) compared to pulp. The highest amounts of flavan-3-ols were present in grape seed collected from the Pune region (2016.84 ± 14.73 mg/kg in Sauvignon blanc and 1945.06 ± 32.69 mg/kg in Shiraz). The concentration of stilbenes was the highest in grape skin (0.13 ± 0.52 to 5.78 ± 5.45 mg/kg) compared to seed and pulp of both varities. Hydroxybenzoic acid (vanillin), hydroxycinnamic acid (p-coumaric acid) and anthocyanins (oenin, malvidin, cyanidin and kuromanin) were found only in Shiraz variety. The results of antioxidant activity (FRAP and DPPH assay) indicated the highest scavenging activity in seed (978.64 ± 56.23 to1133.38 ± 143.65 µMol TE/g DW FRAP and 594.93 ± 37.94 to 631.94 ± 56.45 µMol TE/g DW in DPPH). The phenolic contents in Sauvignon blanc and Shiraz grapes between Pune and Nasik regions did not have any significant difference.
KeywordsGrape Sauvignon blanc Shiraz Phenolic profile LC–MS/MS Antioxidant activity
The authors gratefully acknowledge the funding supports from Indian Council for Agricultural Research (ICAR), New Delhi, India.
- Arts ICW, Hollman PCH (2005) Polyphenols and disease risk in epidemiologic studies1-4. Am J ClinNutr 81:317S–325SGoogle Scholar
- Benmeziane F, Djamai R, Cadot Y, Seridi R (2014) Optimization of extraction parameters of phenolic compounds from Algerian fresh table grapes (Vitisvinifera). Food Res Int 21:1025–1029Google Scholar
- Butkhupl L, Chowtivannakul S, Gaensakoo R, Prathepha P, Samappito S (2010) Study of the phenolic composition of Shiraz red grape cultivar (Vitisvinfera L.) Cultivated in North-eastern Thailand and its antioxidant and antimicrobial activity. S Afr J Enol 31:89–98Google Scholar
- Hismath I, Want Aida WM, Ho CW (2011) Optimization of extraction conditions for phenolic compounds from neem (Azadirachtaindica) leaves. Int Food Res J 18:931–939Google Scholar
- Li Zheng, Pan Qiuhong, Cui Xiangyun, Duan Changqing (2010) Optimization on anthocyanins extraction from wine grape skins using orthogonal test design. Food Sci Bio technol 19:1047–1053Google Scholar
- Pang B, Zhu Y, Lu L, Gu F, Chen H (2016) The applications and features of liquid chromatography-mass spectrometry in the analysis of traditional chinese medicine. Evid Based Complement Altern Med 2016:3837270Google Scholar
- Rolle L, Guidoni S (2007) Color and anthocyanin evaluation of red winegrapes CIE L*, a*, b* parameters. J Int Sci Vigne Vin 41:193–201Google Scholar
- Roobha JJ, Saravanakumar M, Aravindhan KM, Devi PS (2011) The effect of light, temperature, pH on stability of anthocyanin pigments in Musa acuminata bract. Res Plant Biol 1:05–12Google Scholar
- Uma DB, Ho CW, Wan Aida WM (2010) Optimization of extraction parameters of total phenolic compounds from henna (Lawsoniainermis) Leaves. Sains Malays 39:119–128Google Scholar
- Wissam Z, Ghada B, Wassim A, Warid K (2012) Effective extraction of polyphenols and proanthocyanidins from pomegranate’s peel. Int J Pharm Pharm Sci 4:675–682Google Scholar