A non-toxic approach to assess total antioxidant capacity (TAC) of exotic tropical fruits from Thailand
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A simple flow injection analysis (FIA) integrating with a metal-free approach for total antioxidant capacity (TAC) was developed. The non-toxic reaction was based on generating a vibrant blue radical from imipramine to avoid the potential interferents arising from the colorful fruit extracts. The blue radical can be rapidly scavenged by antioxidant compounds present in the sample. TAC values of Thai tropical fruit extracts were assessed by monitoring the quenching in absorbance of the test mixture following the addition of the antioxidant compounds/fruit extracts. The FIA co-operated in order to increase the sample throughput. The results demonstrated that Antidesma thwaiteaianum Muell. Arg. has the highest capacity followed by Terminalia chebula Retz. and Phyllanthus Emblica Linn., respectively. An excellent correlation between the proposed method was found with the DPPH assay. The proposed method allowed the TAC determination of fruit extracts in a high-throughput and straightforward way in accordance with the principles of green analytical chemistry.
KeywordsA non-toxic approach Flow-based analysis Total antioxidant capacity (TAC) Imipramine Tropical fruits
This research was supported by The Thailand Research Fund (TRF) in the project of Thai Fruits-Functional Fruits, Project Number RDG5420028. Topic: Total Antioxidant Capacity of Thai North East’s Fruits, Project Manager: Ms. Sirirat Panich. The authors also would like to acknowledge Faculty of Science and Technology, Rajamangala University of Technology Phra Nakhon.
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Conflict of interest
The authors declare that they have no conflict of interest.
- International Standard, ISO 14502-1:2005(E). (2005) Determination of substance characteristic of green and black tea-Part 1: Content of total polyphenols in tea-Colorimetric method using Folin-Ciocalteu reagent. https://www.iso.org/standard/31356.html. Accessed 22 Nov 2018
- Kaprasob R, Kerdchoechuen O, Laohakunjit N, Thumthanaruk B, Shetty K (2018) Changes in physico-chemical, astringency, volatile compounds and antioxidant activity of fresh and concentrated cashew apple juice fermented with Lactobacillus plantarum. J Food Sci Technol 55:3979–3990CrossRefGoogle Scholar
- Panich S (2018a) A novel assay for evaluation of the total antioxidant capacity (TAC) using a nontoxic probe. Thai J Pharm Sci 42:21–26Google Scholar
- Rumainum IM, Worarad K, Srilaong V, Yamane K (2018) Fruit quality and antioxidant capacity of six Thai mango cultivars. Agric Nat Resour 52:208–214Google Scholar