Development of high yielding strain of Pleurotus tuber-regium: fructification, nutritional and phylogenetic studies

  • Comfort Olukemi BamigboyeEmail author
  • Julius Kola Oloke
  • Joanna Felicity Dames
Original Article


Mushrooms are nutritionally rich, healthy and medicinal. Pleurotus tuber-regium (Fr.) is one of the nutritious medicinal mushroom found in the tropics and subtropics, but with history of slow growth and low sclerotia yield. In this study, mutants were created by mycelia exposure to ultraviolet irradiation (at a wavelength of 254 nm and a distance of 45 cm), for 3 h and sub-cultured at 30 min interval. The DNA from the wild and mutant strains were extracted, PCR amplified and sequenced. A phylogenetic tree was constructed to show the degree of similarity and differences between the wild and the mutant strains. Fructification studies were conducted on Rhodes grass straw and sawdust to determine the viability of the mutant strains and any nutritional improvement. The wild strain of P. tuber-regium and mutant produced at 30 min (Pt30) cultivated on sawdust and Rhodes straw, yielded sclerotia with biological efficiency of 8.8 and 47.6% respectively. Proximate analysis of the sclerotium showed that the mutant, Pt30, had improved nutritional compositions compared to the wild strain with a total non-structural carbohydrate concentration of 2.41 g as against 0.93 g. Conclusively in this study, better strains of P. tuber-regium were produced with faster growth rate, higher mycelia ramification rate on lignocellulosic substrate and a higher sclerotia yield than the wild P. tuber-regium. It was also established that mutagenesis is capable of improving P. tuber-regium for a successful commercial venture in sclerotia production.


Mushroom improvement Sclerotium cultivation Mutagenesis DNA nucleotide sequence Proximate analysis 



The authors are grateful to Organization of Women in Science for the developing world (OWSD) for granting additional funding for the proximate analysis. In addition, one of the authors, Bamigboye, C.O. is grateful to OWSD for the postgraduate fellowship award given to her which was utilized in Rhodes University, and also to LAUTECH, Ogbomoso for granting the study leave.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Department of Biochemistry and MicrobiologyRhodes UniversityGrahamstownSouth Africa
  2. 2.Microbiology Unit, Department of Pure and Applied BiologyLadoke Akintola University of TechnologyOgbomosoNigeria

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