Journal of Food Science and Technology

, Volume 56, Issue 7, pp 3205–3214 | Cite as

Optimization of process parameters on hydroxymethylfurfural content, diastase and invertase activity of coriander honey

  • Rajni KambojEmail author
  • Rubrinder Singh Sandhu
  • R. S. S. Kaler
  • Manab Bandhu Bera
  • Vikas Nanda
Original Article


Floral authenticity of coriander (Coriandrum sativum) honey samples was confirmed by melissopalynology. Effect of temperature, time and pH on quality parameters i.e. hydroxymethylfurfural (HMF) content, diastase and invertase activity of coriander honey was analysed using response surface methodology. Central composite rotatable design was adopted for optimization of process variables. An increased in HMF content was observed with increase in temperature and pH whereas diastase activity decreased with increase in temperature and with a pH value other than the optimum value of 4.6–5.6. Invertase activity was maximum at 4.8 pH. Interaction effect of temperature and pH was significant for HMF whereas interaction effect of temperature and time was significant for HMF, diastase and invertase activity. Optimization of variables was done by the mathematical method, and optimized values of HMF content, diastase, and invertase activity were obtained as 7.78 (mg/kg), 17.95 DN and 13.96 IN, respectively at 47.5 °C (temperature), 4.7 (pH) and 9 min (time).


Diastase HMF Honey Invertase RSM 



The first author is thankful to local beekeepers of Rajasthan (India) for providing honey samples.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.


  1. Atanassova J, Kondova V (2004) Pollen and chemical-physical analysis of unifloral honey from different regions of Bulgaria. Phytol Balc 10(1):45–50Google Scholar
  2. Babacan S, Rand AG (2005) Purification of amylase from honey. J Food Sci 70:413–418CrossRefGoogle Scholar
  3. Babacan S, Rand AG (2007) Characterization of honey amylase. J Food Sci 72:50–55CrossRefGoogle Scholar
  4. Babacan S, Pivarnik LF, Rand AG (2002) Honey amylase activity and food starch degradation. J Food Sci 67:1625–1630CrossRefGoogle Scholar
  5. Barra MPG, Ponce-Diaz MC, Venegas-Gallegos C (2010) Volatile compounds in honey produced in the central valley of Nuble Province, Chile. Chile J Agric Res 70:75–84Google Scholar
  6. Bath PK, Singh N (1999) A comparison between Helianthus annuus and Eucalyptus lanceolatus honey. Food Chem 67:389–397CrossRefGoogle Scholar
  7. Bath PK, Singh N (2000) A research note chemical changes in Helianthus annuus and Eucalyptus lanceolatus honey during storage. J Food Qual 23:443–451CrossRefGoogle Scholar
  8. Bibi S, Husain SZ, Malik RM (2008) Pollen analysis and heavy metals detection in honey samples from seven selected countries. Pak J Bot 40:507–516Google Scholar
  9. Bogdanov S, Jurendic T, Sieber R (2008) Honey for nutrition and health: a review. J Am Coll Nutr 27:677–689CrossRefGoogle Scholar
  10. Vaughn M, Bryant Jr (2001) Palynology Laboratory, Texas A and M University, College Station, Texas 77843–4352, USA. CAP Newsl 24:10–24Google Scholar
  11. Castro-Vazquez L, Diaz-Maroto MC, Perez-Coello MS (2007) Aroma composition and new chemical markers of Spanish citrus honeys. Food Chem 103:601–606CrossRefGoogle Scholar
  12. Codex Alimentarius Commission (2001) Revised standard for honey. Codex Standard 12-1981. Rev 1 (1987), Rev 2 (2001). FAO, RomeGoogle Scholar
  13. European Economic Community (2002) EEC Council Directive 2001/110/EC of 20 December 2001 relating to honey. Off J Eur Commun 110:47–52Google Scholar
  14. Fallico B, Zappala M, Arena E, Verzera A (2004) Effects of conditioning on HMF content in unifloral honeys. Food Chem 85:305–313CrossRefGoogle Scholar
  15. Ghoshdastidar N, Chakrabarti J (1992) Studies on hydroxymethylfurfural formation during storage of honey. J Food Sci Technol 29:399–400Google Scholar
  16. Hasan SH (2013) Effect of storage and processing temperature on honey quality. J Babylon Univ Pure Appl Sci 21:2244–2253Google Scholar
  17. International Honey Commission (2009) Harmonized methods of the International Honey Commission.
  18. Karabournioti S, Zervalaki P (2001) The effect of heating on honey HMF and invertase. Apiacta 36:177–181Google Scholar
  19. Khuri AI, Cornell JA (1987) Response surfaces design and analysis. Marcel Dekker Inc, New YorkGoogle Scholar
  20. Nafea EA, Gumgumjee NM, Danial EN, Hajair AS (2014) Physiochemical and antimicrobial properties of four Egyptian honeys with reference to American foul Brood disease. Life Sci J 11:41–46Google Scholar
  21. Nanda V, Singh S, Raina CS, Jindal N, Singh K, Saxena DC (2004) Optimization of the process variables for the preparation of processed paneer using response surface methodology. Eur Food Res Technol 218:529–534CrossRefGoogle Scholar
  22. Nanda V, Bera MB, Bakhshi AK (2006) Optimization of the process parameters to establish the quality attributes of hydroxymethylfurfural content and diastase activity of sunflower (Helianthus annus) honey using response surface methodology. Eur Food Res Technol 222:64–70CrossRefGoogle Scholar
  23. Nayik GA, Dar BN, Nanda V (2016) Optimization of the process parameters to establish the quality attributes of DPPH radical scavenging activity, total phenolic content, and total flavonoid content of apple (Malus domestica) honey using response surface methodology. Int J Food Prop 19:1738–1748CrossRefGoogle Scholar
  24. Ramirez Cervantes MA, Gonzalez Novelo SA, Sauri Duch E (2000) Effect of the temporary thermic treatment of honey on variation of the quality of the same during storage. Apiacata 35:162–170Google Scholar
  25. Ruoff K, Bogdanov S (2004) Authenticity of honey and other bee products. Apiacta 38:317–327Google Scholar
  26. Sacan O, Yanardag R (2012) Purification and some properties of rose (Fructus cynosbati) hips invertase. Indian J Biochem Biophy 49:109–114Google Scholar
  27. Shapla UM, Solayman M, Alam N, Khalil MI, Gan SH (2018) 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health. Chem Cent J 12:35. CrossRefGoogle Scholar
  28. Singh N, Bath PK (1997) Quality evaluation of different types of Indian honey. Food Chem 58:129–133CrossRefGoogle Scholar
  29. Singh B, Panesar PS, Nanda V, Kennedy JF (2010) Optimization of osmotic dehydration process of carrot cubes in mixtures of sucrose and sodium chloride solutions. Food Chem 123:590–600CrossRefGoogle Scholar
  30. Takenaka T, Echigo T (1974) Changes in enzyme activity during the storage of honey. Bull Fac Agric Tamagawa Univ 14:19–25Google Scholar
  31. Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, Dogan M, Kayacier A (2013) Quality characterization of artisanal and retail Turkish blossom honeys: determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crop Prod 46:124–131CrossRefGoogle Scholar
  32. Tosi E, Ciappini M, Re E, Lucero H (2002) Honey thermal treatment effects on hydroxymethylfurfural (HMF) content. Food Chem 77:71–74CrossRefGoogle Scholar
  33. Visquert M, Escriche I, Perez-Campos A, Fito P (2004) Effects of heat treatment on quality parameters of various honeys. Alimentaria 352:65–72Google Scholar
  34. Von der Ohe W, Persano Oddo L, Piana ML, Morlot M, Martin P (2004) Harmonized methods of melissopalynology. Apidologie 35:S18–S25CrossRefGoogle Scholar
  35. White JW, Kushnir I, Subers MH (1964) Effect of storage and processing temperature on honey quality. Food Technol 18:153–156Google Scholar
  36. Yilmaz H, Kufrevioglu I (2001) Composition of honeys collected from Eastern and South-Eastern Anatolia and effect of storage on hydroxymethylfurfural content and diastase activity. Turk J Agric For 25:347–349Google Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Department of Food TechnologyCh. Devi Lal State Institute of Engineering and TechnologyPanniwala MotaIndia
  2. 2.Department of Food Science and TechnologyGuru Nanak Dev UniversityAmritsarIndia
  3. 3.Department of Food Engineering and TechnologySant Longowal Institute of Engineering and TechnologyLongowalIndia

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