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Journal of Food Science and Technology

, Volume 56, Issue 4, pp 2233–2243 | Cite as

In silico optimization of enzyme mediated debittering of Assam lemon: biochemical and sensory evaluation studies

  • Debajyoti Kundu
  • Sandipan Karmakar
  • Rintu BanerjeeEmail author
Original Article
  • 57 Downloads

Abstract

Commercialization of citrus fruit juice is always hindered by the bitterness development in juice when stored for a significant period of time. In order to debitter citrus juice, an attempt has been taken up by treating the juice with tannase. Central Composite Design (CCD) based Response Surface Methodology (RSM) has been implemented to evaluate and optimize the effect of underlying process parameters viz., enzyme volume, temperature, incubation time and enzyme titre on debittering effect of Assam lemon juice. The significance of parameters and their interaction were assessed by analysis of variance at 95% level of confidence. Optimization study reveals that the maximum debittering (40.12 ± 0.02%) of Assam lemon juice takes place at ambient temperature (37 °C) within an incubation time of 2 h and 1.12% (v/v) enzyme volume while 30 IU/ml enzyme activity. Moreover, percentage contribution of the underlying process parameters demonstrate that the enzyme volume and enzyme titre as first and second most significant contributors in process of debittering. As part of validating the above results, experimental debittering has been performed and compared with predicted debittering percentage which showed a high coefficient value (0.971) which ensures the effectiveness of the proposed model. Biochemical analysis of the treated juice reveals improved antioxidant property after enzymatic treatment by 15.30%. Total sugar and reducing sugar content has also been enhanced by 1.38 and 1.49 folds, respectively, after enzymatic treatment of juice. Furthermore, no alteration in the elemental composition of the treated juice ensure that the quality of the final juice is retained with the enzyme applications. Sensory analysis based on nine-point Hedonic scale advocates the best organoleptic property in 1% (v/v) enzyme treated juice.

Keywords

Assam lemon Debittering Response surface methodology Antioxidant property Elemental composition 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13197_2019_3710_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 14 kb)

References

  1. Aguilar CN, Rodríguez R, Gutiérrez-Sánchez G, Augur C, Favela-Torres E, Prado-Barragan LA, Ramírez-Coronel A, Contreras-Esquivel JC (2007) Microbial tannases: advances and perspectives. Appl Microbiol Biotechnol 76:47–59CrossRefGoogle Scholar
  2. Badhani B, Sharma N, Kakkar R (2015) Gallic acid: a versatile antioxidant with promising therapeutic and industrial applications. RSC Adv 5:27540–27557CrossRefGoogle Scholar
  3. Bhaumik R, Mondal NK (2016) Optimizing adsorption of fluoride from water by modified banana peel dust using response surface modelling approach. Appl Water Sci 6:115–135CrossRefGoogle Scholar
  4. Busto MD, Meza V, Ortega N, Perez MM (2007) Immobilization of naringinase from Aspergillus niger CECT 2088 in poly (vinyl alcohol) cryogels for the debittering of juices. Food Chem 104:1177–1182CrossRefGoogle Scholar
  5. Chaisawadi S, Aiemphasit W, Chommanard N, Kulamai S (1998) Debittering of lime juices with food additives. J Assoc Med Sci 25:65–70Google Scholar
  6. Chakraborty S, Rao PS, Mishra HN (2015) Response surface optimization of process parameters and fuzzy analysis of sensory data of high pressure–temperature treated pineapple puree. J Food Sci 80(8):E1763–E1775CrossRefGoogle Scholar
  7. Dash A, Kundu D, Das M, Bose D, Adak S, Banerjee R (2016) Food biotechnology: a step towards improving nutritional quality of food for Asian countries. Recent Pat Biotechnol 10:43–57CrossRefGoogle Scholar
  8. de Lima JS, Cruz R, Fonseca JC, de Medeiros EV, Maciel MHC, Moreira KA, Motta CMS (2014) Production, characterization of tannase from Penicillium montanense URM 6286 under SSF using agroindustrial wastes, and application in the clarification of grape juice (Vitis vinifera L.). Sci World J 182025:1–9CrossRefGoogle Scholar
  9. Eneji IS, Nurain AA, Salawu OW (2015) Trace metal levels in some packaged fruit juices sold in Makurdi metropolis markets Nigeria. Chem Search J 6(2):42–49Google Scholar
  10. Gamez-Meza N, Noriega-Rodriguez J, Medina-Juarez L, Ortega-Garcia J, Cazarez-Casanova R, Angulo-Guerrero O (1999) Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. J Am Oil Chem Soc 76:1445–1447CrossRefGoogle Scholar
  11. Ganesan B, Perumal P, Manickam VB, Mummudi S, Gotteti SD, Srikakolapu SR, Thirumurthy LS (2010) Quantitative estimation of gallic acid in Triphala Churnam tablet by RP-HPLC. Der Pharma Chem 2(3):19–24Google Scholar
  12. Ghanem F (2012) Juice debittering: basic science, optimization, and recent advances. In: ASME 2012 citrus engineering conference CEC2012-5701Google Scholar
  13. Gujjala LKS, Bandyopadhyay TK, Banerjee R (2016) Kinetic modelling of laccase mediated delignification of Lantana camara. Bioresour Technol 212:47–54CrossRefGoogle Scholar
  14. Hadi TA, Banerjee R, Bhattacharyya BC (1994) Optimization of tannase biosynthesis Rhizopus oryzae. Bioprocess Eng 11:239–243CrossRefGoogle Scholar
  15. Haggerman AE, Butler LG (1978) Protein precipitation method for determination of tannins. J Agric Food Chem 26:809–812CrossRefGoogle Scholar
  16. Hedge JE, Hofreiter BT (1962) Determination of reducing sugars and carbohydrates In: Whistler RL, Be Miller JN (eds) Carbohydrate chemistry, vol 17. Academic Press, New YorkGoogle Scholar
  17. Horticultural Statistics at a Glance (2017) Horticulture statistics division department of agriculture, cooperation and farmers welfare ministry of agriculture and farmers welfare Government of India, 2017. http://nhb.gov.in/statistics/Publication/Horticulture%20At%20a%20Glance%202017%20for%20net%20uplod%20(2).pdf. Accessed 1 July 2018
  18. Hu Q, Wang T, Zhou M, Xue J, Luo Y (2016) In vitro antioxidant-activity evaluation of gallic-acid-grafted chitosan conjugate synthesized by free-radical-induced grafting method. J Agric Food Chem 64:5893–5900CrossRefGoogle Scholar
  19. Iibuchi S, Minoda Y, Yamada K (1967) Studies on tannin acyl hydrolase of microorganisms: part II. A new method of determining the enzyme activity using change in the ultraviolet absorption. Agric Biol Chem 31:513–518Google Scholar
  20. Kapoor A, Iqbal H (2013) Efficiency of tannase produced by Trichoderma Harzianum MTCC 10841 in pomegranate juice clarification and natural tannin degradation. Int J Biotechnol Bioeng Res 4(6):641–650Google Scholar
  21. Kar B, Banerjee R (2000) Biosynthesis of tannin acyl hydrolase from tannin-rich forest residue under different fermentation conditions. J Ind Microbiol Biotechnol 25(1):29–38CrossRefGoogle Scholar
  22. Kundu D, Singh J, Das M, Rastogi A, Banerjee R (2018) A sustainable process for nutrient enriched fruit juice processing: an enzymatic venture. In: Kuila A, Sharma V (eds) Principle and applications of fermentation technology. Scrivener Publishing LLC, Beverly, pp 387–400CrossRefGoogle Scholar
  23. Lawless HT, Popper R, Kroll BJ (2010) A comparison of the labeled magnitude (LAM) scale, an 11-point category scale and the traditional 9 point hedonic scale. Food Qual Prefer 21:4–12CrossRefGoogle Scholar
  24. Malinda K, Sutanto H, Darmawan A (2017) Characterization and antioxidant activity of gallic acid derivative. In: AIP conference proceedings, vol 1904(1)Google Scholar
  25. Mukherjee G, Banerjee R (2003) Production of gallic acid. Biotechnological routes (part 1). Chim Oggi Chem Today 21(1–2):59–62Google Scholar
  26. Mukherjee G, Banerjee R (2006) Effects of temperature, pH and additives on the activity of tannase produced by a co-culture of Rhizopus oryzae and Aspergillus foetidus. World J Microbiol Biotechnol 22(3):207–212CrossRefGoogle Scholar
  27. Patil MB, Dhake AB (2014) Debittering of citrus fruit juice by naringinase of Penicillium purpurogenum. Int J Eng Res Sci Technol 3(2):266–270Google Scholar
  28. Premi BR, Lal BB, Joshi VK (1994) Distribution pattern of bittening principle in Kinnow fruits. J Food Sci Technol 31(2):140–141Google Scholar
  29. Purohit JS, Dutta JR, Nanda RK, Banerjee R (2006) Strain improvement for tannase production from co-culture of Aspergillus foetidus and Rhizopus oryzae. Bioresour Technol 97(6):795–801CrossRefGoogle Scholar
  30. Rajak RC, Banerjee R (2015) Enzymatic delignification: an attempt for lignin degradation from lignocellulosic feedstock. RSC Adv 5:75281–75291CrossRefGoogle Scholar
  31. Ranganna V (1986) Handbook of analysis and quality control of fruit and vegetable products. Tata McGra-Hill Publishing Company Limited, New Delhi, pp 84–85Google Scholar
  32. Ranote PS, Bains GS (1982) Juice of Kinnow fruit. Indian Food Pack 36(5):23–33Google Scholar
  33. Rout S, Banerjee R (2006) Production of tannase under mSSF and its application in fruit juice debittering. Indian J Biotechnol 5:351–356Google Scholar
  34. Sadhukhan B, Mondal NK, Chattoraj S (2014) Biosorptive removal of cationic dye from aqueous system: a response surface methodological approach. Clean Technol Environ Policy 16:1015–1025CrossRefGoogle Scholar
  35. Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. J Agric Food Chem 40:945–948.  https://doi.org/10.1021/jf00018a005 CrossRefGoogle Scholar
  36. Somogyi N (1944) Analytical procedures no. 1, School of Biological Technology, Australia 2033, p 37Google Scholar
  37. Yao J, Guo GS, Ren GH, Liu YH (2014) Production, characterization and applications of tannase. J Mol Catal B Enzym 101:137–147CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Debajyoti Kundu
    • 2
  • Sandipan Karmakar
    • 1
  • Rintu Banerjee
    • 2
    Email author
  1. 1.Xavier Institute of ManagementXavier UniversityBhubaneswarIndia
  2. 2.Department of Agricultural and Food EngineeringIndian Institute of Technology KharagpurKharagpurIndia

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