Malting process has minimal influence on the structure of arabinan-rich rhamnogalacturonan pectic polysaccharides from chickpea (Cicer arietinum L.) hull
- 40 Downloads
The objective of the study was to determine the changes brought about by malting/germination on the pectic polysaccharides (PP’s), the major components of soluble fibres present in chickpea (Cicer arietinum L.) hull. Chickpea hull PP’s were extracted sequentially using ammonium oxalate (AO) and ethylenediaminetetraacetic acid (EDTA), and a comparative study was conducted in native (unprocessed, N–PP) and after subjecting to 48 h malting process (M–PP). Malting process did not show a significant change in the respective yields of AO and EDTA extracted pectic polysaccharides. The degree of esterification of N–PP–EDTA through Fourier transform infrared spectroscopy was found to be five times (~ 21%) more than N–PP–AO (~ 4%). AO isolated PP’s have more complexed xylogalacturonan with relatively more galactan side chains compared to EDTA isolated PPs. Proton (1H) nuclear magnetic resonance result further suggested the occurrence of arabinan rich rhamnogalacturonan in chickpea hull and malting process showed no significant changes in structure.
KeywordsChickpea hull Pectic polysaccharides Malting Arabinan Rhamnogalacturonan NMR
Bovine serum albumin
Total dietary fibre
Degree of esterification
The authors would like to thank the Director, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysuru for the constant encouragement. Ms Shakuntala S., thanks Indian Council of Medical Research, New Delhi, for the Senior Research Fellowship.
- Desalegn BB (2015) Effect of soaking and germination on proximate composition, mineral bioavailability and functional properties of chickpea flour. Food Public Health 5:108–113Google Scholar
- Devries JW (2004) Dietary fiber: the influence of definition on analysis and regulation. J AOAC Int 87:682–706Google Scholar
- Megat RMR, Noralizia CW, Azrina A, Zulkhairi A (2011) Nutritional changes in germinated legumes and rice varieties. Int Food Res J 18:705–713Google Scholar
- Megat RMR, Azrina A, Norhaizan ME (2016) Effect of germination on total dietary fibre and total sugar in selected legumes. Int Food Res J 23:257–261Google Scholar
- Rumiyati R, James AP, Jayasena V (2012) Effect of germination on the nutritional and protein profile of Australian Sweet Lupin (Lupinusangustifolius L.). Food Nutr Sci 3:621–626Google Scholar
- Selvendran RR, O’Neill MA (1987) Isolation and analysis of cell walls from plant material. Methods Biochem Anal 32:25–153Google Scholar
- Vasishtha H, Srivastava RP (2013) Effect of soaking and germination on dietary fibre constituents of chickpea (Cicer arietinum L.). Legume Res 36:174–179Google Scholar