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Malting process has minimal influence on the structure of arabinan-rich rhamnogalacturonan pectic polysaccharides from chickpea (Cicer arietinum L.) hull

  • Shakuntala Sathyanarayana
  • Keelara Veerappa Harish PrashanthEmail author
Original Article
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Abstract

The objective of the study was to determine the changes brought about by malting/germination on the pectic polysaccharides (PP’s), the major components of soluble fibres present in chickpea (Cicer arietinum L.) hull. Chickpea hull PP’s were extracted sequentially using ammonium oxalate (AO) and ethylenediaminetetraacetic acid (EDTA), and a comparative study was conducted in native (unprocessed, N–PP) and after subjecting to 48 h malting process (M–PP). Malting process did not show a significant change in the respective yields of AO and EDTA extracted pectic polysaccharides. The degree of esterification of N–PP–EDTA through Fourier transform infrared spectroscopy was found to be five times (~ 21%) more than N–PP–AO (~ 4%). AO isolated PP’s have more complexed xylogalacturonan with relatively more galactan side chains compared to EDTA isolated PPs. Proton (1H) nuclear magnetic resonance result further suggested the occurrence of arabinan rich rhamnogalacturonan in chickpea hull and malting process showed no significant changes in structure.

Keywords

Chickpea hull Pectic polysaccharides Malting Arabinan Rhamnogalacturonan NMR 

Abbreviations

MHDP

Metahydroxydiphenyl

AO

Ammonium oxalate

PP

Pectic polysaccharides

N

Native

M

Malted

BSA

Bovine serum albumin

TDF

Total dietary fibre

DE

Degree of esterification

NSP

Non-starch polysaccharides

Notes

Acknowledgements

The authors would like to thank the Director, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysuru for the constant encouragement. Ms Shakuntala S., thanks Indian Council of Medical Research, New Delhi, for the Senior Research Fellowship.

Supplementary material

13197_2019_3600_MOESM1_ESM.doc (362 kb)
Supplementary material 1 (DOC 363 kb)

References

  1. Begum R, Yusof YA, Aziz MG, Uddin MB (2017) Structural and functional properties of pectin extracted from jackfruit (Artocarpus heterophyllus) waste: effects of drying. Int J Food Prop 20:190–201CrossRefGoogle Scholar
  2. Beldman G, Osuga D, Whitaker JR (1996) Some characteristics of β-d-xylopyranosidases, α-l-arabinofuranosidases and an arabinoxylan α-l-arabinofuranohydrolase from wheat bran and germinated wheat. J Cereal Sci 23:169–180CrossRefGoogle Scholar
  3. Benítez V, Cantera S, Aguilera Y (2013) Impact of germination on starch, dietary fiber and physicochemical properties in non-conventional legumes. Food Res Int 50:64–69CrossRefGoogle Scholar
  4. Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484–489CrossRefGoogle Scholar
  5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  6. Chung HJ, Park SM, Kim HR (2002) Cloning the gene encoding acetyl xylan esterase from Aspergillus ficuum and its expression in Pichia pastoris. Enzyme Microb Technol 31:384–391CrossRefGoogle Scholar
  7. Desalegn BB (2015) Effect of soaking and germination on proximate composition, mineral bioavailability and functional properties of chickpea flour. Food Public Health 5:108–113Google Scholar
  8. Devries JW (2004) Dietary fiber: the influence of definition on analysis and regulation. J AOAC Int 87:682–706Google Scholar
  9. Ghumman A, Kaur A, Singh N (2016) Impact of germination on flour, protein and starch characteristics of lentil (Lens culinari) and horsegram (Macrotyloma uniflorum L.) lines. LWT Food Sci Technol 65:137–144CrossRefGoogle Scholar
  10. Hagerman AE, Austin PJ (1986) Continuous spectrophotometric assay for plant pectin methyl esterase. J Agric Food Chem 34:440–444CrossRefGoogle Scholar
  11. Khattak AB, Zeb A, Bibi N, Khalil SA, Khattak MS (2007) Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicerarietinum L.) sprouts. Food Chem 104:1074–1079CrossRefGoogle Scholar
  12. Kyomugasho C, Christiaens S, Shpigelman A (2015) FT-IR spectroscopy, a reliable method for routine analysis of the degree of methylesterification of pectin in different fruit- and vegetable-based matrices. Food Chem 176:82–90CrossRefGoogle Scholar
  13. Le Goff A, Renard C, Bonnin E, Thibault JF (2001) Extraction, purification and chemical characterisation of xylogalacturonans from pea hulls. Carbohydr Polym 45:325–334CrossRefGoogle Scholar
  14. Lopez-Martinez LX, Leyva-Lopez N, Gutierrez-Grijalva EP, Heredia JB (2017) Effect of cooking and germination on bioactive compounds in pulses and their health benefits. J Fun Food 38:624–634CrossRefGoogle Scholar
  15. Manrique GD, Lajolo FM (2002) FT-IR spectroscopy as a tool for measuring degree of methyl esterification in pectins isolated from ripening papaya fruit. Postharvest Biol Technol 25:99–107CrossRefGoogle Scholar
  16. Martín-Cabrejas MA, Díaz MF, Aguilera Y (2008) Influence of germination on the soluble carbohydrates and dietary fibre fractions in non-conventional legumes. Food Chem 107:1045–1052CrossRefGoogle Scholar
  17. Mckelvy J, Lee YC (1969) Microheterogeneity of the carbohydrate group of Aspergillus oryzae a-amylase 1, 2, 3. Arch Biochem Biophys 132:99–110CrossRefGoogle Scholar
  18. Megat RMR, Noralizia CW, Azrina A, Zulkhairi A (2011) Nutritional changes in germinated legumes and rice varieties. Int Food Res J 18:705–713Google Scholar
  19. Megat RMR, Azrina A, Norhaizan ME (2016) Effect of germination on total dietary fibre and total sugar in selected legumes. Int Food Res J 23:257–261Google Scholar
  20. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  21. Miyamoto A, Chang KC (1992) Extraction and physicochemical characterization of pectin from sunflower head residues. J Food Sci 57:1439–1443CrossRefGoogle Scholar
  22. Muralikrishna G, Tharanathan RN (1994) Characterization of pectic polysaccharides from pulse husks. Food Chem 50:87–89CrossRefGoogle Scholar
  23. Nirmala M, Rao MVSSTS, Muralikrishna G (2000) Carbohydrates and their degrading enzymes from native and malted finger millet (Ragi, Eleusine coracana, Indaf-15). Food Chem 69:175–180CrossRefGoogle Scholar
  24. Orfila C, Knox JP (2000) Spatial regulation of pectic polysaccharides in relation to pit fields in cell walls of tomato fruit pericarp. Plant Physiol 122:775–782CrossRefGoogle Scholar
  25. Rumiyati R, James AP, Jayasena V (2012) Effect of germination on the nutritional and protein profile of Australian Sweet Lupin (Lupinusangustifolius L.). Food Nutr Sci 3:621–626Google Scholar
  26. Sawardeker JS, Sloneker JH, Jeanes A (1965) Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Anal Chem 37:1602–1604CrossRefGoogle Scholar
  27. Schmelter T, Wientjes R, Vreeker R, Klaffke W (2002) Enzymatic modifications of pectins and the impact on their rheological properties. Carbohydr Polym 47:99–108CrossRefGoogle Scholar
  28. Selvendran RR, O’Neill MA (1987) Isolation and analysis of cell walls from plant material. Methods Biochem Anal 32:25–153Google Scholar
  29. Shiga TM, Lajolo FM (2006) Cell wall polysaccharides of common beans (Phaseolus vulgaris L.)—composition and structure. Carbohydr Polym 63:1–12CrossRefGoogle Scholar
  30. Subba Rao MVSST, Muralikrishna G (2001) Non-starch polysaccharides and bound phenolic acids from native and malted finger millet (Ragi, Eleusine coracana, Indaf - 15). Food Chem 72:187–192CrossRefGoogle Scholar
  31. Urbano G, López-Jurado M, Frejnagel S (2005) Nutritional assessment of raw and germinated pea (Pisum sativum L.) protein and carbohydrate by in vitro and in vivo techniques. Nutrition 21:230–239CrossRefGoogle Scholar
  32. Vasishtha H, Srivastava RP (2013) Effect of soaking and germination on dietary fibre constituents of chickpea (Cicer arietinum L.). Legume Res 36:174–179Google Scholar
  33. Wang W, Ma X, Jiang P (2016) Characterization of pectin from grapefruit peel: a comparison of ultrasound-assisted and conventional heating extractions. Food Hydrocoll 61:730–739CrossRefGoogle Scholar
  34. Winning H, Viereck N, Nørgaard L (2007) Quantification of the degree of blockiness in pectins using 1H NMR spectroscopy and chemometrics. Food Hydrocoll 21:256–266CrossRefGoogle Scholar
  35. Wood JA, Knights EJ, Campbell GM, Choct M (2014) Differences between easy- and difficult-to-mill chickpea (Cicer arietinum L.) genotypes. Part I: broad chemical composition. J Sci Food Agric 94:1437–1445CrossRefGoogle Scholar
  36. Woolard GR, Rathbone EB, Novellie L (1977) Studies on free sugars, water soluble gums and hemicelluloses from barley grains and malts. J Sci Food Agric 28:469–476CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Shakuntala Sathyanarayana
    • 1
  • Keelara Veerappa Harish Prashanth
    • 1
    Email author
  1. 1.Functional Biopolymer Lab, Department of BiochemistryCSIR-Central Food Technological Research InstituteMysoreIndia

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