A sensitive multiplex PCR protocol for simultaneous detection of chicken, duck, and pork in beef samples

  • Panzhu Qin
  • Wei Qu
  • Jianguo XuEmail author
  • Dongqing Qiao
  • Li Yao
  • Feng Xue
  • Wei ChenEmail author
Original Article


A rapid and sensitive multiplex PCR assay was developed for simultaneous identification of the adulteration ingredients of chicken, duck and pork in beef. Specific primers for the mitochondrial genes of Cyt b, CO III, ATPase subunit 8/6 and Cyt b of chicken, duck, pork, and beef, respectively, were adopted in the assay. DNA exaction from meat samples was carried out by using magnetic nanoparticles as rapid separation substrates. The multiplex PCR assay showed that the limit of detection was 0.05% for each species. Moreover, the multiplex PCR specifically identified five beef samples adulterated with pork and one beef samples adulterated with chicken among the 35 commercial samples examined, indicating the practicability of this multiplex PCR method for identifying adulterated ingredients of chicken, duck, and pork in commercial beef products.


Multiplex PCR Identification Chicken Duck Pork Beef Meat adulteration Sensitivity Specificity 



This work is financially supported by the Grant of 2017YFF0208600, China Agriculture Research System-48 (CARS-48), Anhui Provincial Modern Argo-industry Tech. Research System (NYCYTX-2016-84), NSFC 21475030 and the National 10000 Talents-Youth Top-notch Talent Program.

Supplementary material

13197_2019_3591_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1378 kb)


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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.School of Food and Biological Engineering, Engineering Research Center of Bio-Process, MOEHefei University of TechnologyHefeiChina
  2. 2.School of Biological and Medical EngineeringHefei University of TechnologyHefeiChina
  3. 3.College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina

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