Effect of karwanda (Carissa congesta Wight) and sugar addition on physicochemical characteristics of ash gourd (Benincasa hispida) and bottle gourd (Langenaria siceraria) based beverages

  • P. PurohitEmail author
  • S. Palamthodi
  • S. S. Lele
Original Article


There is a clear trend towards increasing consumption of juices as they can reduce imbalance of redox potential and provide necessary health benefits to consumers. Levels of karwanda (Carissa congesta Wight) and vegetable juices were varied to prepare nine different formulations of ash gourd-karwanda (AgK) and bottle gourd-karwanda blends (BgK) of higher nutritive, sensory qualities and storability. Total polyphenols (TP), antioxidant activity (AOA), total soluble solids and acidity were increased significantly (p ≤ 0.05) with addition of karwanda. AgK blend (35:35) and BgK blend (35:30) were selected based on their higher overall acceptability, TP and AOA. AgK blends had higher α-amylase (31%) while BgK blends had higher α-glucosidase (43%) inhibitory activities. Concentration of TP and anthocyanins decreased significantly (p < 0.05), AOA remained unchanged and anti-inflammatory activities decreased (33–38%) in AgK and BgK blends during accelerated storage at 50 °C for 12 days. Addition of sugar in BgK blend decreased stability of TP (11%), flavonoids (31%) and anthocyanins (8%). During in vitro gastrointestinal digestion, TP, flavonoids and anthocyanins reduction rate was significantly higher for BgK blend with sugar.


Karwanda Blended beverage AgK BgK TP AOA 



The authors are thankful to University Grant Commission, India for their financial support.

Supplementary material

13197_2019_3570_MOESM1_ESM.pdf (29 kb)
Supplementary material 1 (PDF 29 kb)


  1. Abeysinghe DC, Li X, Sun C et al (2007) Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chem 104:1338–1344. CrossRefGoogle Scholar
  2. Ayala-Zavala JF, Wang SY, Wang CY, González-Aguilar GA (2004) Effect of storage temperatures on antioxidant capacity and aroma compounds in strawberry fruit. LWT - Food Sci Technol 37:687–695. CrossRefGoogle Scholar
  3. Barnuud NN, Zerihun A, Gibberd M, Bates B (2014) Berry composition and climate: responses and empirical models. Int J Biometeorol. Google Scholar
  4. Dewanto V, Xianzhong W, Adom KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010–3014. CrossRefGoogle Scholar
  5. Evrendilek GA, Jin Z, Ruhlman K et al (2000) Microbial safety and shelf-life of apple juice and cider processed by bench and pilot scale PEF systems. Innov Food Sci Emerg Technol 1:77–86. CrossRefGoogle Scholar
  6. Ghule BV, Ghante MH, Saoji AN, Yeole PG (2006) Hypolipidemic and antihyperlipidemic effects of Lagenaria siceraria (Mol.) fruit extracts. Indian J Exp Biol 44:905–909Google Scholar
  7. Hubbermann EM, Heins A, Stöckmann H, Schwarz K (2006) Influence of acids, salt, sugars and hydrocolloids on the colour stability of anthocyanin rich black currant and elderberry concentrates. Eur Food Res Technol 223:83–90. CrossRefGoogle Scholar
  8. Itankar PR, Lokhande SJ, Verma PR et al (2011) Antidiabetic potential of unripe Carissa carandas Linn. fruit extract. J Ethnopharmacol 135:430–433. CrossRefGoogle Scholar
  9. Jayachandran LE, Chakraborty S, Rao PS (2015) Effect of high pressure processing on physicochemical properties and bioactive compounds in litchi based mixed fruit beverage. Innov Food Sci Emerg Technol 28:1–9. CrossRefGoogle Scholar
  10. Kevers C, Falkowski M, Tabart J et al (2007) Evolution of antioxidant capacity during storage of selected fruits and vegetables. J Agric Food Chem 55:8596–8603. CrossRefGoogle Scholar
  11. Majumdar TK, Wadikar DD, Bawa AS (2012) Development and storage stability of aseptically processed ashgourd-mint leaves juice. Int Food Res J 19:823–828Google Scholar
  12. Morales-de la Peña M, Salvia-Trujillo L, Rojas-Graü MA, Martín-Belloso O (2010) Impact of high intensity pulsed electric field on antioxidant properties and quality parameters of a fruit juice-soymilk beverage in chilled storage. LWT - Food Sci Technol 43:872–881. CrossRefGoogle Scholar
  13. Neilson AP, Ferruzzi MG (2011) Influence of formulation and processing on absorption and metabolism of flavan-3-ols from tea and cocoa. Annu Rev Food Sci Technol 2:125–151. CrossRefGoogle Scholar
  14. Oboh G, Ademosun AO, Akinleye M et al (2015) Starch composition, glycemic indices, phenolic constituents, and antioxidative and antidiabetic properties of some common tropical fruits. J Ethn Foods 2:64–73. CrossRefGoogle Scholar
  15. Ortega N, Reguant J, Romero MP et al (2009) Effect of fat content on the digestibility and bioaccessibility of cocoa polyphenol by an in vitro digestion model. J Agric Food Chem 59:5743–5749. CrossRefGoogle Scholar
  16. Patil RP, Pai SR, Pawar NV et al (2012) Chemical characterization, mineral analysis, and antioxidant potential of two underutilized berries (Carissa carandus and Eleagnus conferta) from the Western Ghats of India. Crit Rev Food Sci Nutr 52:312–320. CrossRefGoogle Scholar
  17. Pérez-ramírez IF, Castaño-tostado E, Ramírez-de León JA et al (2015) Effect of stevia and citric acid on the stability of phenolic compounds and in vitro antioxidant and antidiabetic capacity of a roselle (Hibiscus sabdariffa L.) beverage. Food Chem 172:885–892. CrossRefGoogle Scholar
  18. Raj D, Sharma PC, Vaidya D (2011) Effect of blending and storage on quality characteristics of blended sand pear-apple juice beverage. J Food Sci Technol 48:102–105. CrossRefGoogle Scholar
  19. Reshma AKP, Brindha P (2014) In vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum. Asian J Pharm Clin Res 7:121–129Google Scholar
  20. Ribnicky DM, Roopchand DE, Oren A et al (2014) Effects of a high fat meal matrix and protein complexation on the bioaccessibility of blueberry anthocyanins using the TNO gastrointestinal model (TIM-1). Food Chem 142:349–357. CrossRefGoogle Scholar
  21. Rodríguez-Roque MJ, Rojas-Graü MA, Elez-Martínez P, Martín-Belloso O (2013) Changes in vitamin C, phenolic, and carotenoid profiles throughout in vitro gastrointestinal digestion of a blended fruit juice. J Agric Food Chem 61:1859–1867. CrossRefGoogle Scholar
  22. Serra A, Macià A, Romero M-P et al (2010) Bioavailability of procyanidin dimers and trimers and matrix food effects in in vitro and in vivo models. Br J Nutr 103:944–952. CrossRefGoogle Scholar
  23. Sharma A, Agarwal PK, Deep S (2010) Characterization of different conformations of bovine serum albumin and their propensity to aggregate in the presence of N-cetyl- N, N, N-trimethyl ammonium bromide. J Colloid Interface Sci 343:454–462. CrossRefGoogle Scholar
  24. Singh A, Uppal GK (2015) A review on Carissa carandas: phytochemistry, ethno-pharmacology, and micropropagation as conservation strategy. Asian J Pharm Clin Res 8:26–30Google Scholar
  25. Singh JP, Kaur A, Shevkani K, Singh N (2016) Composition, bioactive compounds and antioxidant activity of common Indian fruits and vegetables. J Food Sci Technol. Google Scholar
  26. Sonawane SK, Arya SS (2015) Effect of drying and storage on bioactive components of jambhul and wood apple. J Food Sci Technol. Google Scholar
  27. Sravani VJ, Ravi N, Roopa N et al (2017) Use of high pressure technology for the development of novel jam and its quality evaluation during storage. J Food Sci Technol. Google Scholar
  28. Subramanian R, Asmawi MZ, Sadikun A (2008) In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Polon 55:391–398Google Scholar
  29. Temiz H, Kezer G (2015) Effects of fat replacers on physicochemical, microbial and sensorial properties of Kefir made using mixture of cow and goat’s milk. J Food Process Preserv 39:1421–1430. CrossRefGoogle Scholar
  30. Tiwari AK (2014) Revisiting “vegetables” to combat modern epidemic of imbalanced glucose homeostasis. Pharmacogn Mag 10:S207–S213. CrossRefGoogle Scholar
  31. Vaz JA, Barros L, Martins A et al (2011) Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions. Food Chem 126:610–616. CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  1. 1.Food Engineering and Technology DepartmentInstitute of Chemical TechnologyMatunga, MumbaiIndia

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