Optimization of microwave-assisted extraction of bioactive compounds from New Zealand and Chinese Asparagus officinalis L. roots
The extraction of total phenolics (TPC), total flavonoids content (TFC), total saponins content (TSC), and caffeic acid (AC) contents of asparagus roots extract (ARE) from New Zealand and Chinese AR cultivars was optimized following a microwave-assisted extraction combined with central composite design. The determination of AC was conducted by HPLC in samples extracted under the optimum extraction conditions. The optimal variables for ethanol extraction generated a maximum TPC, TFC and TSC of optimal results for 68.6 mg GAE/g, 11.9 mg RE/g and 0.7 mg SE/g as well as antioxidant power towards β-carotene bleaching assay (%βsc) (57.2%), superoxide anion radical (%O sc 2− ) scavenging capacity (20.1%) and ferric reducing antioxidant power assay (FRAP) (1.63 µmol/g). For methanol, optimum extraction conditions obtained maximum TPC (62.6 mg GAE/g) TFC (10.7 mg RE/g), TSC (0.68 mg SE/g) with %βsc (53.9%), %O sc 2− (19.1%) and FRAP (0.63 µmol/g). The content of caffeic acid from ARE ranged from 0.46 to 2.89 mg/g with ethanol and from 0.41 to 2.64 mg/g with methanol.
KeywordsAsparagus officinalis L. Roots Antioxidants Caffeic acid MAE Cultivars
The financial support from the research Grant (Ref. 2014BAD04B00) of the Food Science and Pharmacy College of Xinjiang Agriculture University, China) to this project is highly appreciated. The authors would also like to thank the Dr. Xiaohong Wang of the Department of Veterinary Medicine for providing the facilities for this project.
- Fang Y (2005) Purification and monosaccharide composition of saponin from Asparagus officianlis L. Chin J Biotechnol 21:446–450Google Scholar
- Ghimire BK, Seong ES, Kim EH, Ghimeray AK, Yu CY, Ghimire BK, Chung IM (2011) A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal. J Med Plant Res 5:1884–1891Google Scholar
- Hossain M, Sharmin FA, Akhter S, Bhuiyan MA, Shahriar M (2012) Investigation of cytotoxicity and in-vitro antioxidant activity of asparagus racemosus root extract. Int Curr Pharm J 1:250–257Google Scholar
- Huang XF, Zhang Y, Kong LY (2006) Chemical constituents of Asparagus officinalis. Chin J Nat Med 4:181–184Google Scholar
- Jain N, Goyal S, Ramawat K (2011) Evaluation of antioxidant properties and total phenolic content of medicinal plants used in diet therapy during postpartum healthcare in Rajasthan. Int J Pharm Pharm Sci 3:248–253Google Scholar
- Li J, Nie L, Liu M, Hu S (2015) The study of asparagus different parts’ active ingredients. Food Res Technol 15:006Google Scholar
- Shah S, Dhanani T, Kumar S (2013) Comparative evaluation of antioxidant potential of extracts of Vitex negundo, Vitex trifolia, Terminalia bellerica, Terminalia chebula, Embelica officinalis and Asparagus racemosus. Innov J Pharm Pharm Sci 1:44–53Google Scholar