Effect of supercritical carbon dioxide processing on Vibrio parahaemolyticus in nutrient broth and in oysters (Crassostrea gigas)
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This study aimed to evaluate the technical feasibility of supercritical carbon dioxide (sc-CO2) treatment for Vibrio parahaemolyticus inactivation in oysters (Crassostrea gigas) and in nutrient broth. For this purpose, a variable-volume reactor was used as experimental system and a 23 factorial design was adopted considering the mass ratio between carbon dioxide and the product, pressurization and depressurization rate and pressurization cycles. Through statistical analysis of the experimental data, the mass ratio of 1:0.8 (product:carbon dioxide), depressurization rate of 10.0 MPa/min and one cycle of pressurization was determined as the best process condition to eliminate V. parahaemolyticus, and this was the condition used for the inactivation kinetic analysis. Comparison between the inactivation kinetics of V. parahaemolyticus showed that the behavior of this microorganism inactivation depends on the environment in which it operates and its initial count. The results confirm that the supercritical carbon dioxide is effective in inactivating microorganisms in oysters, including pathogenic V. parahaemolyticus, demonstrating the potential of this technology in the food industry.
KeywordsOysters Vibrio parahaemolyticus Supercritical carbon dioxide Inactivation
This work was supported by the Brazilian financial support agencies CNPq, CAPES and FINEP.
- APHA - American Public Health Association (2001) Standard methods for the examination of water and wastewater, 20th edn. APHA, American Water Works Association (AWWA) and Water Environment Federation (WEF), Baltimore, MDGoogle Scholar
- Aragão GMF, Corradini MG, Normand MD, Peleg M (2007) Evaluation of the weibull and log normal distribution functions as survival models of Escherichia coli under isothermal and non isothermal conditions. Int J Food Microb 119:243–257. https://doi.org/10.1016/j.ijfoodmicro.2007.08.004 CrossRefGoogle Scholar
- Ceni G, Silva MF, Junior Valério C, Cansian RL, Oliveira JV, Rosa CD, Mazutti M (2016) Continuous inactivation of alkaline phosphatase and Escherichia coli in milk using compressed carbon dioxide as inactivating agent. J CO2 Utilizat 13:24–28. https://doi.org/10.1016/j.jcou.2015.11.003 CrossRefGoogle Scholar
- FDA - United States Food and Drug Administration (2009) National Shellfish sanitation program (NSSP) guide for the control of molluscan shellfish: 2015 revision. https://www.fda.gov/Food/GuidanceRegulation/FederalStateFoodPrograms/ucm2006754.htm. Accessed 24 Sep 2017
- Garcia-Gonzalez L, Geeraerd AH, Spilimbergo K, Elst K, van Ginneken L, Debevere J, Impe JF, van Devlieghere F (2007) High pressure carbon dioxide inactivation of microorganisms in foods: the past, the present and the future. Int J Food Microb 117:1–28. https://doi.org/10.1016/j.ijfoodmicro.2007.02.018 CrossRefGoogle Scholar
- Garcia-Gonzalez L, Geeraerd AH, Elst K, van Ginneken L, Impe JF, van Devlieghere JF (2009) Inactivation of naturally occurring microorganisms in liquid whole egg using high pressure carbon dioxide processing as an alternative to heat pasteurization. J Supercrit Fluids 51:74–82. https://doi.org/10.1016/j.supflu.2009.06.020 CrossRefGoogle Scholar
- Jay JM (2000) Modern food microbiology. In: Gaithersburg M (ed) 6th edn. Aspen PublishersGoogle Scholar
- Lee RJ, Rangdale RE, Croci L, Hervio-Heath D, Lozach S (2008) Bacterial pathogens in seafood. In: Bùrresen Torger (ed) Improving seafood products for the consumer. CRC Press LLC, Boca RatonGoogle Scholar
- Lin H-M, Cao N, Chen L-F (1994) Antimicrobial effect of pressurized carbon dioxide on Listeria monocytogenes. J Food Sci 59:657–659. https://doi.org/10.1111/j.1365-2621.1994.tb05587.x CrossRefGoogle Scholar
- Sikin AM, Walkling-Ribeiro M, Rizvi SSH (2016) Synergistic effect of supercritical carbon dioxide and peracetic acid on microbial inactivation in shredded Mozzarella-type cheese and its storage stability at ambient temperature. Food Control 70:174–182. https://doi.org/10.1016/j.foodcont.2016.05.050 CrossRefGoogle Scholar
- Silva JM, Rigo AA, Dalmolin IA, Debien I, Cansian RL, Oliveira JV, Mazutti MA (2013) Effect of pressure, depressurization rate and pressure cycling on the inactivation of escherichia coli by supercritical carbon dioxide. Food Control 29:76–81. https://doi.org/10.1016/j.foodcont.2012.05.068 CrossRefGoogle Scholar